Abstract：
:An Escherichia coli K-12 biofilm was grown at a dilution rate of 0.028 h(-1) for 48 h in a glucose-limited chemostat coupled to a modified Robbins' device to determine its susceptibility to infection by bacteriophage T4. Bacteriophage T4 at a multiplicity of infection (MOI) of 10 caused a log reduction in biofilm density (expressed as colony forming units (CFU) per cm2) at 90 min postinfection. After 6 h, a net decrease and equilibrium in viral titer was seen. When biofilms were exposed to T4 phage at a MOI of 100, viral titer doubled after 90 min. After 6 h, viral titers (expressed as plaque forming units (PFU) per cm2) stabilized at levels approximately one order of magnitude higher than seen at a MOI of 10. Scanning confocal laser microscopy images also indicated disruption of biofilm morphology following T4 infection with the effects being more pronounced at a MOI of 100 than at a MOI of 10. These results imply that biofilms under carbon limitation can act as natural reservoirs for bacteriophage and that bacteriophage can have some influence on biofilm morphology.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Corbin BD,McLean RJ,Aron GMsubject
Has Abstractpub_date
2001-07-01 00:00:00pages
680-4issue
7eissn
0008-4166issn
1480-3275journal_volume
47pub_type
杂志文章abstract：:To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a me...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-096
更新日期：2005-12-01 00:00:00
abstract：:The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to q...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-024
更新日期：2005-06-01 00:00:00
abstract：:Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0528
更新日期：2015-12-01 00:00:00
abstract：:Environmental scanning electron microscopic observations of Postia placenta grown on a defined medium and on red spruce wood allowed for the examination of the hydrated sheath of P. placenta. In the wood environment, mature hyphae that were not adhering to the substrate were observed to have a mycofibrillar morphology...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-058
更新日期：1995-04-01 00:00:00
abstract：:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-131
更新日期：2005-03-01 00:00:00
abstract：:Campylobacter spp. are a substantial cause of gastroenteritis worldwide. Human infection can result from ingestion of contaminated food or water from a variety of sources, including the consumption of fresh produce that is contaminated with the pathogen via the use of contaminated irrigation water. Using molecular met...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0710
更新日期：2016-05-01 00:00:00
abstract：:Viruses were isolated from every sample of raw (100 L) and treated (1000 L) water collected at a water treatment plant drawing sewage-contaminated river water. Few plaque-forming isolates were formed but cytopathogenic viruses were isolated as frequently in drinking water as in raw water. In drinking water some sample...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-063
更新日期：1981-04-01 00:00:00
abstract：:Isoquinoline is a nitrogen heterocyclic compound that is associated with coal- and oil-derived wastes. Four strains of bacteria able to degrade isoquinoline in pure culture were isolated from sites known to be contaminated with oil. Isoquinoline was used as the sole source of carbon and nitrogen by these isolates. Iso...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-089
更新日期：1994-07-01 00:00:00
abstract：:A transformation protocol mediated by Agrobacterium tumefaciens is described for the sapstaining fungus Ophiostoma piceae. We compared transformants obtained from Agrobacterium with those obtained from yeast-like cells made into spheroplasts and treated with CaCl2. For all putative transformants analyzed, Southern hyb...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-036
更新日期：2003-04-01 00:00:00
abstract：:Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-126
更新日期：1975-06-01 00:00:00
abstract：:Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-153
更新日期：1978-08-01 00:00:00
abstract：:Arsenic hyperaccumulator fern Pteris vittata L. produces large amounts of root exudates that are hypothesized to solubilize arsenic and maintain a unique rhizosphere microbial community. Total heterotrophic counts on rich or defined media supplemented with up to 400 mmol/L of arsenate showed a diverse arsenate-resista...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-005
更新日期：2010-03-01 00:00:00
abstract：:The effect of mechanical wounding or foliar diseases caused by Sclerotinia homoeocarpa or Rhizoctonia solani on the epiphytic yeast communities on creeping bentgrass and tall fescue were determined by leaf washing and dilution plating. Total yeast communities on healthy bentgrass and tall fescue leaves ranged from 7.9...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-073
更新日期：2004-10-01 00:00:00
abstract：:Antibiotic susceptibilities of 382 strains of Campylobacter jejuni isolated in Alberta, Canada, in 1980 and 1981 were determined. Although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. Tetracycline resistance was noted in 6.8% of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-006
更新日期：1986-01-01 00:00:00
abstract：:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:Citrate synthase (citrate-oxaloacetate lyase (CoA acetylating), EC 4.1.3.7) has been purified to electrophoretic homogeneity from a marine Pseudomonas. The enzyme was made up of identical subunits, with a molecular wieght of about 53 000, as determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis. T...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-039
更新日期：1978-03-01 00:00:00
abstract：:A moderately halophilic Acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. Maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2M NaCl or 1M KCl. Two kinds of amylase, amylases I and II were purified from the culture fil...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-169
更新日期：1978-09-01 00:00:00
abstract：:The introduction of the 7-valent pneumococcal vaccine (PCV7) in Canada was very effective in reducing invasive pneumococcal disease (IPD) in children; however, increases of non-PCV7 serotypes have subsequently offset some of these reductions. A 13-valent pneumococcal vaccine (PCV13) targeting additional serotypes was ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0614
更新日期：2013-12-01 00:00:00
abstract：:Formate dehydrogenase activity (EC 1.2.1.2) has been demonstrated in cell-free preparations of Mycobacterium phlei by following the reduction of 2,6 dichlorophenolindophenol. thiazolyl blue tetrazolium, or equine cytochrome c. The reduction of equine cytochrome c was inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide. N...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-018
更新日期：1977-02-01 00:00:00
abstract：:It is important to understand the dynamics of tetracycline-resistant bacteria (TRB) and tetracycline resistance genes (TRGs) in bulk and rhizosphere soils for evaluating the spread of TRGs from pig manure to human. In this work, a greenhouse experiment was conducted to investigate the difference in abundance of TRB, t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0767
更新日期：2017-07-01 00:00:00
abstract：:One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-029
更新日期：2001-05-01 00:00:00
abstract：:Eight Enterococcus faecium strains isolated from ewe milk and artisanal cheese from northwest Argentina were screened for biotechnological properties relevant to flavour development. The API ZYM test showed absence of proteases, presence of high amounts of peptidases, and high esterase-lipase activities. Low extracell...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-136
更新日期：2006-03-01 00:00:00
abstract：:A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-162
更新日期：1997-12-01 00:00:00
abstract：:Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involv...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-032
更新日期：2010-06-01 00:00:00
abstract：:The effects of seven thiophosphorus pesticides and their degradation products on estuarine ammonium oxidizers were examined. Using an axenic, closed-culture, high cell density assay, the pesticides caused little inhibition of ammonium oxidation. However, the degradation product of methyl parathion, p-aminophenol, sign...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-216
更新日期：1980-11-01 00:00:00
abstract：:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-147
更新日期：1991-11-01 00:00:00
abstract：:Seven tk- mutants of herpes simplex virus, type 2 (HSV-2), and three tk- mutants of herpes simplex virus, type 1 (HSV-1), were isolated which did not produce the thymidine kinase (TK) polypeptides but formed smaller polypeptides not seen in wild-type infected cells. Positive TK mRNA selection by hybridization to the c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-063
更新日期：1983-04-01 00:00:00
abstract：:Two rod-shaped viruses of similar length occurring in hops were isolated from a mixed infection. One (hop mosaic virus) infected Nicotiana clevelandii, produced mosaic symptoms in mosaic-sensitive hop cultivars, and did not infect Chenopodium murale. The other (hop latent virus) infected C. murale but not N. cleveland...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-168
更新日期：1976-08-01 00:00:00
abstract：:Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-021
更新日期：1989-01-01 00:00:00
