Abstract:
:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurospora crassa, encoding the catabolic dehydroquinase, as a selectable marker with a double-mutant host strain, auxotrophic for aromatic amino acids, integration of the plasmid was observed to be predominantly at ectopic chromosomal sites. Cotransformation with the qa-2+ gene and a plasmid containing a heat shock gene sequence (hsp70 of N. crassa) suggested integration site preference. High efficiencies of transformation to hygromycin resistance were achieved employing the bacterial hygromycin B phosphotransferase gene with N. crassa, the patulin-producer Penicillium urticae, and the causal agent of blackleg disease of crucifers, Leptosphaeria maculans. The economically important species Aspergillus oryzae was similarly transformed to benomyl resistance with the benomyl-resistant beta-tubulin gene of N. crassa as a dominant selectable marker.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Chakraborty BN,Patterson NA,Kapoor Mdoi
10.1139/m91-147subject
Has Abstractpub_date
1991-11-01 00:00:00pages
858-63issue
11eissn
0008-4166issn
1480-3275journal_volume
37pub_type
杂志文章abstract::An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-251
更新日期:1975-11-01 00:00:00
abstract::A large pool of antibiotic resistant and auxotrophic mutants was isolated from the Staphylococcus aureus phage group 2 strains UT0002-19 and UT0017 by (1) antibiotic gradient plates, (2) trimethoprim selection, and (3) nitrosoguanidine mutagenesis, which sometimes was coupled by enrichment with either penicillin or me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-161
更新日期:1980-08-01 00:00:00
abstract::The morphology of Clostridium pasteurianum cells grown on 10(-2) M SO32- showed significant alteration in cell shape and the absence of the electron translucent reserve polysaccharide (amylopectin) when compared to sulfate-grown cells. At the lower sulfite concentrations (10(-3) and 10(-4)M SO32-) the cells showed the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-036
更新日期:1976-02-01 00:00:00
abstract::Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-093
更新日期:2009-11-01 00:00:00
abstract::Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-064
更新日期:1989-03-01 00:00:00
abstract::Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-218
更新日期:1977-10-01 00:00:00
abstract::We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-102
更新日期:2007-01-01 00:00:00
abstract::Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-063
更新日期:1979-03-01 00:00:00
abstract::Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, G...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-071
更新日期:2010-10-01 00:00:00
abstract::In the dairy industry, bacterial contaminants persist on equipment surfaces due to spore and biofilm formation. These are involved in cross-contamination problems that affect the quality of processed products and limit their life. The pasteurization process, in which milk is submitted to moderate heat treatment, is in...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/cjm-2018-0435
更新日期:2019-06-01 00:00:00
abstract::The aim of this study was to unravel, by focusing on cell surface properties, the underlying virulence factors contributing to the difference in the pathogenicity observed in two Acinetobacter baumannii strains isolated from the same patient. The two strains were phenotypically different: (i) a mucoid strain (AB-M),...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-131
更新日期:2012-03-01 00:00:00
abstract::The ultraviolet germicidal irradiation (UVGI) dose necessary to inactivate fungal spores on an agar surface and the efficacy of UVGI were determined for cultures of Stachybotrys chartarum (ATCC 208877). This study employed a UVGI testing unit consisting of four chambers with a 9-W, Phillips, low pressure, mercury UVGI...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-061
更新日期:2005-09-01 00:00:00
abstract::The application of crossed immunoelectrophoresis to the analysis of a reference cytoplasm and cell envelope preparation from Pasteurella multocida serotype 1 revealed antigenic complexity not previously found. At least 55 cytoplasmic and 19 cell envelope antigens were clearly distinguished. Variation of anticytoplasm ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-118
更新日期:1980-06-01 00:00:00
abstract::Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-164
更新日期:1980-08-01 00:00:00
abstract::Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involv...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-032
更新日期:2010-06-01 00:00:00
abstract::Auxotrophs in three different races of Pyricularia oryzae were obtained following ultraviolet mutagenesis, and tested for complementarity on minimal medium. Prototrophic growth resulted between combinations of different auxotrophs of single strains, but not between strains. The growth did not result form crossfeeding,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-079
更新日期:1976-04-01 00:00:00
abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-070
更新日期:1996-05-01 00:00:00
abstract::Wild-type glucose uptake and glucose chemotaxis activities were restored in glucose transport defective Pseudomonas aeruginosa strains PFB360 and PFB362 after introduction of plasmid pPZ129, containing a 1.1-kilobase DNA fragment that is essential for the expression of the P. aeruginosa periplasmic glucose binding pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-163
更新日期:1993-11-01 00:00:00
abstract::Examination of immune complexes between cells of Streptococcus mutans and homologous antiserum by the techniques of thin-sectioning and freeze-etching revealed that the cells were embedded within and extensive matrix 80-90 nm thick with defined boundaries. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-129
更新日期:1976-06-01 00:00:00
abstract::Bifidobacteria are generally acknowledged as major gut microflora used as probiotics, which promote human health. In this study, the effects of the administration of Bifidobacterim bifidum on modulating gastrointestinal (GI) tract microflora, acid and bile resistance, and physiological indices in BALB/c mice were inve...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0694
更新日期:2015-02-01 00:00:00
abstract::The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-032
更新日期:1983-02-01 00:00:00
abstract::Cells of five Bacteroides species were examined following treatment with homologous antisera and staining with ruthenium red. They were enveloped by glycocalyces and these extensive fibrous exopolysaccharide matrices were fully retained as an integral "capsule" by some cells, while other cells showed "capsule" as well...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-124
更新日期:1984-06-01 00:00:00
abstract::An investigation of brucellosis caused by Brucella suis, type 4, in reindeer, Rangifer tarandus L., and other ungulates inoculated experimentally with virulent isolates was undertaken to observe the course of infection, follow titres of serum agglutins, and determine the extent to which intraspecific and interspecific...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-024
更新日期:1978-02-01 00:00:00
abstract::Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-063
更新日期:1982-04-01 00:00:00
abstract::The structures of lipopolysaccharides (LPSs) of lic1 and lic1/lic2 mutants from Haemophilus influenzae type b strain Eagan (RM153) were investigated using methylation analysis, electrospray ionization - mass spectrometry, and nuclear magnetic resonance spectroscopy on O-deacylated, O- and N-deacylated core oligosaccha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-009
更新日期:2008-04-01 00:00:00
abstract::Human astrovirus (HAstV) was an important cause of viral gastroenteritis in infants in Wuhan city based on our previous study. The aim of the study was to investigate the nature of HAstV infection in Wuhan, People's Republic of China, especially in adults. Stool specimens were collected from 361 children and 301 adult...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-085
更新日期:2011-11-01 00:00:00
abstract::Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-059
更新日期:2009-09-01 00:00:00
abstract::The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-012
更新日期:1975-01-01 00:00:00
abstract::This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-083
更新日期:2007-10-01 00:00:00
abstract::Mixtures of anionic HgCl3-/HgCl4(2)-complexes were less toxic to terrestrial bacteria (Erwinia herbicola, Agrobacterium tumefaciens), to marine bacteria (Acinetobacter sp., Aeromonas sp.), and to bacteriophages (phi 11 M 15 of Staphylococcus aureus and P1 of Escherichia coli) than were equivalent concentrations of Hg ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-197
更新日期:1979-11-01 00:00:00