Characterization of mutants of herpes simplex viruses, types 1 and 2, that produce fragments of the thymidine kinase polypeptide.

abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...

journal_title：Canadian journal of microbiology

authors： White AP,Allen-Vercoe E,Jones BW,DeVinney R,Kay WW,Surette MG

更新日期：2007-01-01 00:00:00

abstract：:The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size di...

journal_title：Canadian journal of microbiology

authors： Ishiguro EE,Ramey WD

更新日期：1978-06-01 00:00:00

abstract：:Our freshwater caulobacter collection contains about 40 strains that are morphologically similar to Caulobacter crescentus. All elaborate a crystalline protein surface (S) layer made up of protein monomers 100-193 kDa in size. We conducted a comparative study of S-layer secretion in 6 strains representing 3 size group...

journal_title：Canadian journal of microbiology

authors： Iuga M,Awram P,Nomellini JF,Smit J

更新日期：2004-09-01 00:00:00

abstract：:Species of Enterobacter and Serratia were examined for deoxyribonucleic acid relatedness to Klebsielleae, to atypical erwiniae, and to other members of Enterobacteriaceae. Deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. Strains of Enterobacter cl...

journal_title：Canadian journal of microbiology

authors： Steigerwalt AG,Fanning GR,Fife-Asbury MA,Brenner DJ

更新日期：1976-02-01 00:00:00

abstract：:In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...

journal_title：Canadian journal of microbiology

authors： Ishiguro EE

更新日期：1979-10-01 00:00:00

abstract：:Fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of Clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. Enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. With the development of the spore, enterotoxin accumula...

journal_title：Canadian journal of microbiology

authors： Niilo L

更新日期：1977-07-01 00:00:00

abstract：:In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...

journal_title：Canadian journal of microbiology

authors： Yoh M,Frimpong EK,Voravuthikunchai SP,Honda T

更新日期：1999-09-01 00:00:00

abstract：:The presence of killer and proteolytic yeasts was studied among 944 isolates representing 105 species from tropical yeast communities. We found 13 killer toxin producing species, with Pichia kluyveri being the most frequent. Other killer yeast isolates were Candida apis, Candida bombicola, Candida fructus, Candida kru...

journal_title：Canadian journal of microbiology

authors： Abranches J,Morais PB,Rosa CA,Mendonça-Hagler LC,Hagler AN

更新日期：1997-04-01 00:00:00

abstract：:Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...

journal_title：Canadian journal of microbiology

authors： Vala AK,Dave BP,Dube HC

更新日期：2006-06-01 00:00:00

abstract：:Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...

journal_title：Canadian journal of microbiology

authors： Nyhlén L,Unestam T

更新日期：1978-11-01 00:00:00

abstract：:Three distinct bacterial populations have been defined in the bovine rumen: the rumen fluid population; the population associated with food particles; and the population adherent to the rumen epithelium. Alkaline phosphatase activity has been reported in cells of the first two populations and here we report that assay...

journal_title：Canadian journal of microbiology

authors： Fay JP,Cheng KJ,Costerton JW

更新日期：1979-08-01 00:00:00

abstract：:Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...

journal_title：Canadian journal of microbiology

authors： Castric PA

更新日期：1983-10-01 00:00:00

abstract：:The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...

journal_title：Canadian journal of microbiology

authors： Doolittle WF

更新日期：1988-04-01 00:00:00

abstract：:We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...

journal_title：Canadian journal of microbiology

authors： Xiang H,Wei GF,Jia S,Huang J,Miao XX,Zhou Z,Zhao LP,Huang YP

更新日期：2006-11-01 00:00:00

abstract：:In Neurospora crassa, heat shock results in the induction of 9 to 11 heat shock proteins (HSP), of which HSP80 is the most abundant and the first to be synthesized. The induction of HSP80 was investigated during normal growth (2% sucrose) and under sucrose starvation. Transfer of mycelium to a medium supplemented with...

journal_title：Canadian journal of microbiology

authors： Roychowdhury HS,Kapoor M

更新日期：1988-02-01 00:00:00

abstract：:Candida albicans is a dimorphic yeast capable of producing alternate morphological forms (yeast or mycelium) in response to environmental changes. The intracellular level of glutathione, which helps to maintain the redox potential of the cell, is decreased significantly during the thermal induction of yeast-to-myceliu...

journal_title：Canadian journal of microbiology

authors： Manavathu M,Gunasekaran S,Porte Q,Manavathu E,Gunasekaran M

更新日期：1996-01-01 00:00:00

abstract：:The structures of lipopolysaccharides (LPSs) of lic1 and lic1/lic2 mutants from Haemophilus influenzae type b strain Eagan (RM153) were investigated using methylation analysis, electrospray ionization - mass spectrometry, and nuclear magnetic resonance spectroscopy on O-deacylated, O- and N-deacylated core oligosaccha...

journal_title：Canadian journal of microbiology

authors： Masoud H,Moxon ER,Richards JC

更新日期：2008-04-01 00:00:00

abstract：:In the dairy industry, bacterial contaminants persist on equipment surfaces due to spore and biofilm formation. These are involved in cross-contamination problems that affect the quality of processed products and limit their life. The pasteurization process, in which milk is submitted to moderate heat treatment, is in...

journal_title：Canadian journal of microbiology

authors： Malek F

更新日期：2019-06-01 00:00:00

abstract：:Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris et P. morganii tryptophanases (TPases) were studied for the spectral forms of the enzyme. The pH effect on the absorption spectrum and on the enzyme specific activity revealed that the coli group TPases are identical with but differ from P...

journal_title：Canadian journal of microbiology

authors： Simard C,Mardini A,Bordeleau LM

更新日期：1975-06-01 00:00:00

abstract：:Asexual sporulation in the fungus Aspergillus nidulans is controlled, in part, by a central regulatory pathway composed of the brlA, abaA, and wetA genes. The coding region of each of these genes was fused, in frame, to the threonine-inducible alcohol dehydrogenase promotor then stably incorporated into the A. nidulan...

journal_title：Canadian journal of microbiology

authors： Sewall TC

更新日期：1994-12-01 00:00:00

abstract：:In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...

journal_title：Canadian journal of microbiology

authors： Chang KP,Musgrave AJ

更新日期：1975-02-01 00:00:00

abstract：:The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with approp...

journal_title：Canadian journal of microbiology

authors： Rezaei M,Rabbani Khorasgani M,Zarkesh Esfahani SH,Emamzadeh R,Abtahi H

更新日期：2020-01-01 00:00:00

abstract：:In Escherichia coli, the N-terminal domain of the essential protein FtsK (FtsKN) is proposed to modulate septum formation through the formation of dynamic and essential protein interactions with both the Z-ring and late-stage division machinery. Using genomic mutagenesis, complementation analysis, and in vitro pull-do...

journal_title：Canadian journal of microbiology

authors： Berezuk AM,Roach EJ,Seidel L,Lo RY,Khursigara CM

更新日期：2020-04-01 00:00:00

abstract：:Considering the medical, biotechnological, and economical importance of actinobacteria, there is a continuous need to improve the tools for genetic engineering of a broad range of these microorganisms. Intergeneric conjugation has proven to be a valuable yet imperfect tool for this purpose. The natural resistance of m...

journal_title：Canadian journal of microbiology

authors： Allard N,Garneau D,Poulin-Laprade D,Burrus V,Brzezinski R,Roy S

更新日期：2015-08-01 00:00:00

abstract：:The production of cold shock proteins (csps) and cold acclimation proteins (caps) was characterized in the psychrotrophic bacterium Pseudomonas putida Q5 and its transconjugant P. putida Q5T which contains the toluene-degradative TOL (pWWO) plasmid, using two-dimensional gel electrophoresis and computing scanning lase...

journal_title：Canadian journal of microbiology

authors： Gumley AW,Inniss WE

更新日期：1996-08-01 00:00:00

abstract：:Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by q...

journal_title：Canadian journal of microbiology

authors： Burt WR,Smith RA

更新日期：1983-01-01 00:00:00

abstract：:New antibiotics produced by Streptomyces echinatus A8331 cultured in the presence of heterocyclic aromatic acids can be separated and purified by high-performance liquid chromatography using reversed phase columns. Natural quinoxaline antibiotics and certain quinoline derivatives can also be efficiently separated in n...

journal_title：Canadian journal of microbiology

authors： Gauvreau D,Waring MJ

更新日期：1984-06-01 00:00:00

abstract：:The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...

journal_title：Canadian journal of microbiology

authors： Nour SM,Cleyet-Marel JC,Beck D,Effosse A,Fernandez MP

更新日期：1994-05-01 00:00:00

abstract：:A defined medium (CDM) is described which supported growth and sporulation of type E strains of Clostridium botulinum, but not sporulation of other serotypes of C. botulinum or C. sporogenes. As compared to growth in complex medium, spore outgrowth was delayed and both the growth rate and the cell yield was reduced. H...

journal_title：Canadian journal of microbiology

authors： Hawirko RZ,Naccarato CA,Lee RP,Maeba PY

更新日期：1979-04-01 00:00:00

abstract：:A Tn551 insertional pleiotropic mutant defective in the production of several exoproteins was isolated from Staphylococcus aureus 196E and characterized. The pleiotropism of the mutant was due to a single insertion of the transposon as evidenced by Southern blot hybridization and by the transfer of its phenotype by tr...

journal_title：Canadian journal of microbiology

authors： Giraudo AT,Raspanti CG,Calzolari A,Nagel R

更新日期：1994-08-01 00:00:00