Abstract:
:The production of cold shock proteins (csps) and cold acclimation proteins (caps) was characterized in the psychrotrophic bacterium Pseudomonas putida Q5 and its transconjugant P. putida Q5T which contains the toluene-degradative TOL (pWWO) plasmid, using two-dimensional gel electrophoresis and computing scanning laser densitometry. Similar growth rates for the psychrotrophic bacterium P. putida Q5 and the transconjugant were found at temperatures ranging from 30 to 0 degree C. Sixteen proteins were quantified and compared in P. putida Q5 and P. putida Q5T following a 25 to 5 degrees C cold shock or constant growth at 5 degrees C. During constant growth at 25 degrees C, a decrease in the synthesis of various proteins occurred in the transconjugant. Following cold shock to 5 degrees C or constant growth at 5 degrees C, csps and caps were produced with a greater number occurring in the transconjugant. This may suggest an additional stress response in the transconjugant owing to metabolic load exerted by the TOL plasmid. Growth of P. putida Q5T with toluate produced seven proteins that appeared to be TOL-plasmid mediated and of which some were also designated as caps.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Gumley AW,Inniss WEdoi
10.1139/m96-100subject
Has Abstractpub_date
1996-08-01 00:00:00pages
798-803issue
8eissn
0008-4166issn
1480-3275journal_volume
42pub_type
杂志文章abstract::Pathogenic and nonpathogenic isolates of Venturia inaequalis were grown in liquid culture. Hyphae were treated with two types of fimbrial antiserum (AU- and AV-1) and examined by immunofluorescent microscopy, in order to establish the distribution of fimbrial epitopes in whole cell mounts. The AV-1 antiserum was speci...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-061
更新日期:2000-09-01 00:00:00
abstract::Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...
journal_title:Canadian journal of microbiology
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更新日期:2012-09-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:2009-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-099
更新日期:1991-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-174
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1981-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-073
更新日期:2004-10-01 00:00:00
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更新日期:2013-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1997-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-043
更新日期:1991-04-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-042
更新日期:2008-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1139/cjm-2014-0211
更新日期:2014-06-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-090
更新日期:1990-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-111
更新日期:1996-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-008
更新日期:1994-01-01 00:00:00
abstract::Environmental scanning electron microscopic observations of Postia placenta grown on a defined medium and on red spruce wood allowed for the examination of the hydrated sheath of P. placenta. In the wood environment, mature hyphae that were not adhering to the substrate were observed to have a mycofibrillar morphology...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-058
更新日期:1995-04-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-148
更新日期:1976-07-01 00:00:00
abstract::Natural enrichments of magnetotactic bacteria were used to study the sites where heavy metals accumulate in uncultured bacteria. Most bacteria obtained by magnetic concentration from these enrichments contained, in addition to the magnetosomes, large phosphorus-rich granules in the cytoplasm. Metal (Zn, Mn, Sr, Cd, Al...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1978-02-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1139/w01-050
更新日期:2001-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-154
更新日期:1997-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-047
更新日期:2002-06-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-209
更新日期:1983-10-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-149
更新日期:1996-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-059
更新日期:1997-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1993-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-187
更新日期:1982-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0708
更新日期:2014-01-01 00:00:00