Outgrowth and sporulation studies on Clostridium botulinum type E: influence of isoleucine.

abstract：:To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a me...

journal_title：Canadian journal of microbiology

authors： Latha JN,Rashmi K,Mohan PM

更新日期：2005-12-01 00:00:00

abstract：:New antibiotics produced by Streptomyces echinatus A8331 cultured in the presence of heterocyclic aromatic acids can be separated and purified by high-performance liquid chromatography using reversed phase columns. Natural quinoxaline antibiotics and certain quinoline derivatives can also be efficiently separated in n...

journal_title：Canadian journal of microbiology

authors： Gauvreau D,Waring MJ

更新日期：1984-06-01 00:00:00

abstract：:The lytic effect of lysozyme on Streptococcus faecalis ATCC 9790 was studied by spectrophotometry and electron microscopy and it was found to be highly dependent on the ionic strength of the suspending media and on the ratio lysozyme to bacterial cell mass. When 7.2 X 10(8) bacteria/mL are exposed to 0.4 mg/mL of lyso...

journal_title：Canadian journal of microbiology

authors： Carvalho ME,Gonçalves MH,Silva MT

更新日期：1984-07-01 00:00:00

abstract：:In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...

journal_title：Canadian journal of microbiology

authors： Franke-Whittle IH,Goberna M,Insam H

更新日期：2009-05-01 00:00:00

abstract：:The effects of the Clostridium difficile toxin were examined in HeLa and mouse adrenal tumor (MAT) cells. Cytotoxicity was evaluated by vital dye exclusion and 51Cr release. In both HeLa and MAT cells, C. difficile toxin caused rounding of virtually 100% of cells. This rounding was distinguishable from rounding produc...

journal_title：Canadian journal of microbiology

authors： Gurwith MJ,Langston C,Dunsmore B

更新日期：1982-01-01 00:00:00

abstract：:Lyme disease is a tick-borne disease that is emerging in Canada. The disease is caused by spirochetes of the Lyme borreliosis group, which is expanding as new species are discovered. In Canada, Lyme disease risk has so far been assessed primarily by detection of Borrelia burgdorferi sensu stricto. Of Ixodes scapularis...

journal_title：Canadian journal of microbiology

authors： Lewis J,Lloyd VK

更新日期：2019-02-01 00:00:00

abstract：:Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...

journal_title：Canadian journal of microbiology

authors： Law DK,Stoltz J,Henderson AM,Tsang RS

更新日期：2005-07-01 00:00:00

abstract：:Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...

journal_title：Canadian journal of microbiology

authors： Evangelista AT,Coppola KM,Furness G

更新日期：1984-08-01 00:00:00

abstract：:Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...

journal_title：Canadian journal of microbiology

authors： Rivero-García PC,Cruz CV,Alonso PS,Vaca S,Negrete-Abascal E

更新日期：2005-10-01 00:00:00

abstract：:During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...

journal_title：Canadian journal of microbiology

authors： McCready RG,Krouse HR

更新日期：1979-12-01 00:00:00

abstract：:Passive protective activities of three different classes of monoclonal antibodies in mice against challenge with strain ATCC 31432 (capsular type I) of Staphylococcus epidermidis were examined. Monoclonal IgM antibody passively protected mice against challenge with the homologous strain, whereas monoclonal IgG1 and Ig...

journal_title：Canadian journal of microbiology

authors： Ichiman Y,Usui Y,Suganuma M,Yoshida K

更新日期：1991-05-01 00:00:00

abstract：:The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...

journal_title：Canadian journal of microbiology

authors： Poonsuk K,Tribuddharat C,Chuanchuen R

更新日期：2014-07-01 00:00:00

abstract：:The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spect...

journal_title：Canadian journal of microbiology

authors： Shibata H,Kobayashi S

更新日期：2006-08-01 00:00:00

abstract：:To develop a library-dependent method of tracking fecal sources of contamination of beaches on the Atlantic coast of southwestern France, a library of 6368 Escherichia coli isolates was constructed from samples of feces, from 40 known human or animal sources collected in the vicinity of Arcachon Bay in 2010, and in Fr...

journal_title：Canadian journal of microbiology

authors： Garabetian F,Vitte I,Sabourin A,Moussard H,Jouanillou A,Mornet L,Lesne M,Lyautey E

更新日期：2020-12-01 00:00:00

abstract：:The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieve...

journal_title：Canadian journal of microbiology

authors： Chen J,Banks D,Jarret RL,Jones JB

更新日期：2000-12-01 00:00:00

abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...

journal_title：Canadian journal of microbiology

authors： Pereira EM,Silva-Queiroz SR,Cabrera Gomez JG,Silva LF

更新日期：2009-06-01 00:00:00

abstract：:Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common ...

journal_title：Canadian journal of microbiology

authors： Pando JM,Pfeltz RF,Cuaron JA,Nagarajan V,Mishra MN,Torres NJ,Elasri MO,Wilkinson BJ,Gustafson JE

更新日期：2017-09-01 00:00:00

abstract：:Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have d...

journal_title：Canadian journal of microbiology

authors： Racagni G,de Lema MG,Hernández G,Machado-Domenech EE

更新日期：1995-10-01 00:00:00

abstract：:Mixtures of anionic HgCl3-/HgCl4(2)-complexes were less toxic to terrestrial bacteria (Erwinia herbicola, Agrobacterium tumefaciens), to marine bacteria (Acinetobacter sp., Aeromonas sp.), and to bacteriophages (phi 11 M 15 of Staphylococcus aureus and P1 of Escherichia coli) than were equivalent concentrations of Hg ...

journal_title：Canadian journal of microbiology

authors： Babich H,Stotzky G

更新日期：1979-11-01 00:00:00

abstract：:Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pat...

journal_title：Canadian journal of microbiology

authors： Yurdusev N,Nicolas JL,Ladire M,Ducluzeau R,Raibaud P

更新日期：1987-03-01 00:00:00

abstract：:The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...

journal_title：Canadian journal of microbiology

authors： Vachon V,Kristjanson DN,Coulton JW

更新日期：1988-02-01 00:00:00

abstract：:5-Methoxymethyl-2'-deoxyuridine (MMUdR), a drug with potent antiviral activity in vitro against Herpes simplex virus, was investigated for its immunosuppressive effects. Doses as high as 2000mg/kg given daily for 9 days were not immunosupporessive as judged by the fact that treated animals produced normal immune respo...

journal_title：Canadian journal of microbiology

authors： Rouse BT,Babiuk LA,Gupta VS

更新日期：1977-08-01 00:00:00

abstract：:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...

journal_title：Canadian journal of microbiology

authors： Chakraborty BN,Patterson NA,Kapoor M

更新日期：1991-11-01 00:00:00

abstract：:The effect of atmospheric composition and storage temperature on growth and survival of uninjured and sublethally heat-injured Escherichia coli O157:H7, inoculated onto brain heart infusion agar containing 0.3% beef extract (BEM), was determined. BEM plates were packaged in barrier bags in air, 100% CO2, 100% N2, 20% ...

journal_title：Canadian journal of microbiology

authors： Semanchek JJ,Golden DA,Williams RC

更新日期：1999-03-01 00:00:00

abstract：:Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...

journal_title：Canadian journal of microbiology

authors： Brilhante RS,Cordeiro RA,Medrano DJ,Monteiro AJ,Sidrim JJ,Rocha MF

更新日期：2005-06-01 00:00:00

abstract：:The PII protein is apparently involved in the control of NifA activity in Herbaspirillum seropedicae. To evaluate the probable role of PII in signal transduction, uridylylation assays were conducted with purified H. seropedicae PII and Escherichia coli GlnD, or a cell-free extract of H. seropedicae as sources of uridy...

journal_title：Canadian journal of microbiology

authors： Benelli EM,Buck M,de Souza EM,Yates MG,Pedrosa FO

更新日期：2001-04-01 00:00:00

abstract：:Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be compos...

journal_title：Canadian journal of microbiology

authors： Casida LE Jr,Liu KC

更新日期：1979-06-01 00:00:00

abstract：:Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both st...

journal_title：Canadian journal of microbiology

authors： O'Reilly T,Rosendal S,Niven DF

更新日期：1984-10-01 00:00:00

abstract：:3-O-Methyl-D-glucose (3-O-MeGlc) or a mixture of 3-O-MeGlc and glucose stimulate invertase, beta-glucosidase, alpha-glucosidase, and alpha-galactosidase production by Cryptococcus laurentii. They also increase invertase and alpha-glucosidase production by Saccharomyces cerevisiae. The stimulatory effect of 3-O-MeGlc i...

journal_title：Canadian journal of microbiology

authors： Bhanot P,Brown RG

更新日期：1980-11-01 00:00:00

abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...

journal_title：Canadian journal of microbiology

authors： Fedorak PM,Coy DL,Salloum MJ,Dudas MJ

更新日期：2002-01-01 00:00:00