Abstract:
:The PII protein is apparently involved in the control of NifA activity in Herbaspirillum seropedicae. To evaluate the probable role of PII in signal transduction, uridylylation assays were conducted with purified H. seropedicae PII and Escherichia coli GlnD, or a cell-free extract of H. seropedicae as sources of uridylylating activity. The results showed that alpha-ketoglutarate and ATP stimulate uridylylation whereas glutamine inhibits uridylylation. Deuridylylation of PII-UMP was dependent on glutamine and inhibited by ATP and alpha-ketoglutarate. PII uridylylation and (or) deuridylylation in response to these effectors suggests that PII is a nitrogen level signal transducer in H. seropedicae.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Benelli EM,Buck M,de Souza EM,Yates MG,Pedrosa FOdoi
10.1139/w01-018subject
Has Abstractpub_date
2001-04-01 00:00:00pages
309-14issue
4eissn
0008-4166issn
1480-3275journal_volume
47pub_type
杂志文章abstract::A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-151
更新日期:1979-09-01 00:00:00
abstract::During exponential growth, strain AW405 of Escherichia coli K-12 did not regulate the fatty acid composition of its lipids in response either to temperature or to the addition of NaCl, KCl, or MgCl2 to the medium. Growth was severely restricted at temperatures below 21 degrees C. Differential scanning calorimetry (DSC...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-128
更新日期:1981-08-01 00:00:00
abstract::We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-064
更新日期:2006-11-01 00:00:00
abstract::Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-204
更新日期:1983-10-01 00:00:00
abstract::In British Columbia, Canada, drinking water is tested for total coliforms and Escherichia coli, but there is currently no routine follow-up testing to investigate fecal contamination sources in samples that test positive for indicator bacteria. Reliable microbial source tracking (MST) tools to rapidly test water sampl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0618
更新日期:2013-03-01 00:00:00
abstract::The multi-drug resistant pathogen Acinetobacter baumannii displays unusual control of its SOS mutagenesis genes, as it does not encode a LexA repressor, but instead employs the UmuDAb repressor and a small DdrR protein that is uniquely found in Acinetobacter species. We used bacterial adenylate cyclase t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2020-0219
更新日期:2020-11-12 00:00:00
abstract::Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-119
更新日期:2005-02-01 00:00:00
abstract::Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Sixty-four species of filamentous fungi from five flare pits in northern and western Canada were tested for their ability to degrade crude oil using gas chromatographic analysis of residual hydrocarbons following incubation. Nine isolates were tested further using radiorespirometry to determine the extent of mineraliz...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-106
更新日期:1983-06-01 00:00:00
abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0399
更新日期:2014-10-01 00:00:00
abstract::Arsenic hyperaccumulator fern Pteris vittata L. produces large amounts of root exudates that are hypothesized to solubilize arsenic and maintain a unique rhizosphere microbial community. Total heterotrophic counts on rich or defined media supplemented with up to 400 mmol/L of arsenate showed a diverse arsenate-resista...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-005
更新日期:2010-03-01 00:00:00
abstract::For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindI...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-047
更新日期:2003-05-01 00:00:00
abstract::Eight Enterococcus faecium strains isolated from ewe milk and artisanal cheese from northwest Argentina were screened for biotechnological properties relevant to flavour development. The API ZYM test showed absence of proteases, presence of high amounts of peptidases, and high esterase-lipase activities. Low extracell...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-136
更新日期:2006-03-01 00:00:00
abstract::Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-058
更新日期:1993-04-01 00:00:00
abstract::A previously unmapped pan gene was localized in an Escherichia coli K-12 strain using Hfr and F' matings and by transduction studies. This Pan- mutation was shown by supplementation studies to impart a block in pantoate biosynthesis. The pan gene cotransduced with rha, metB, and argE placing it at 87 min. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-190
更新日期:1981-11-01 00:00:00
abstract::A study has been undertaken to account for the presence of Thiobacillus acidophilus in iron-grown cultures of Thiobacillus ferrooxidans. Attempts to adapt T. acidophilus to ferrous iron were not successful but the facultative autotroph grew to a limited extent in the spent medium of T. ferrooxidans and was able to gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-178
更新日期:1980-09-01 00:00:00
abstract::Six effective Rhizobium leguminosarum bv. phaseoli strains were examined for nodulation competitiveness on common bean (Phaseolus vulgaris L.), using all possible two-strain combinations of inoculum. Nodule occupancy was determined with strain-specific fluorescent antibodies. The strains were divided into three groups...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-026
更新日期:1992-02-01 00:00:00
abstract::The site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide was assessed by collecting high-resolution phosphorus nuclear magnetic resonance spectra in the presence of manganese, a paramagnetic divalent cation. This technique revealed high-affinity interactions between the cation and all o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-010
更新日期:1986-01-01 00:00:00
abstract::Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involv...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-032
更新日期:2010-06-01 00:00:00
abstract::The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-079
更新日期:2003-10-01 00:00:00
abstract::Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-245
更新日期:1976-11-01 00:00:00
abstract::Ten bacteriophages infecting Micrococcus luteus have been characterized. All phages contain double-stranded DNA, of 64.3--73.5 mol% guanine plus cytosine (GC). The DNA of phage N7 has the highest GC content reported for any bacterial virus. No unusual bases have been found. The intracellular replicating DNAs of six ph...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-158
更新日期:1979-09-01 00:00:00
abstract::Bottles of medium were inoculated with both strains of Chromatiacea and Chlorobiaceae (mixed cultures) and immersed at differnt depths in the marine coastal waters of the Marseilles basin (France). After development, the in vivo absorption characteristics of the cells show the effect of the daylight penetration on the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::Vero cells were more suitable then BHK-21 cells for the detection of rubella-specific IgG antibodies because the nonspecific fluorescence was minimal. However, BHK-21 cells were found more sensitive than Vero cells for the detection of rubella-specific IgM antibodies. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-041
更新日期:1975-03-01 00:00:00
abstract::The goal of this study was to assess the presence and surfactant potential of naturally occurring microbes from a tropical soil with petrochemical contamination. Microorganisms in a soil sample from a Brazilian landfarm were isolated and grown on petroleum as the sole carbon source. Of 60 isolates screened for petrole...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-052
更新日期:2007-08-01 00:00:00
abstract::Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed tha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w07-116
更新日期:2008-02-01 00:00:00
abstract::Fluorescent antibody was prepared against a temperate-soil isolate of Beijerinckia obtained from a rhizosphere of rice growing in Camargue (France). The antibody did not cross-react with any of 6 species of Azotobacter, 4 species of Beijerinckia, or 44 unidentified soil bacteria isolated from a spectrum of rhizosphere...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-023
更新日期:1977-02-01 00:00:00
abstract::Leptospira interrogans serotype pomona was isolated from the kidneys of a normal striped skunk (Mephitis mephitis hudsonicus) collected near Kindersley, Saskatchewan. Although there were no gross lesions, small foci of chronic interstitial nephritis were observed microscopically. This is the first reported isolation o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-237
更新日期:1977-12-01 00:00:00