Abstract:
:The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieved from the GenBank database. At this particular locus, the gene order of tRNA(ala)-tRNA(ile) is conserved among all the studied strains of Xylella and Xanthomonas, and different from those of other bacteria. Sequence analysis showed that Xanthomonas is the most closely related genus. Results from restriction endonuclease analysis suggested the presence of two rrn operons in the genome of a Xylella fastidiosa Pierce's disease strain.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Chen J,Banks D,Jarret RL,Jones JBdoi
10.1139/w00-114subject
Has Abstractpub_date
2000-12-01 00:00:00pages
1171-5issue
12eissn
0008-4166issn
1480-3275journal_volume
46pub_type
杂志文章abstract::Surface oxidation of sulfides and copper (Cu) activation are 2 of the main processes that determine the efficiency of flotation. The present study was developed with the intention to ascertain the role of the phenomena in the biomodification of sulfides by Acidithiobacillus ferrooxidans culture (cells and growth me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-072
更新日期:2012-09-01 00:00:00
abstract::Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-177
更新日期:1983-09-01 00:00:00
abstract::The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-090
更新日期:1990-07-01 00:00:00
abstract::Fluorescent Pseudomonas spp. isolated from fruiting bodies (FB) of Cantharellus cibarius were characterized physiologically and genetically and were compared with fluorescent Pseudomonas from forest soil and with sequences from the GenBank database. Pseudomonas spp. from FB differed physiologically from isolates from ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-062
更新日期:2002-08-01 00:00:00
abstract::A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-027
更新日期:1993-02-01 00:00:00
abstract::Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0703
更新日期:2016-07-01 00:00:00
abstract::Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-107
更新日期:1988-05-01 00:00:00
abstract::Dense populations of anaerobic bacteria were found sequentially layered below the thermocline in two eutrophic lakes in southwest Michigan. Phase and electron microscopy of whole cells and thin sections were used to reveal the in situ morphology of the dominant members of the community. The predominant chlorophyll-con...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-051
更新日期:1975-03-01 00:00:00
abstract::The site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide was assessed by collecting high-resolution phosphorus nuclear magnetic resonance spectra in the presence of manganese, a paramagnetic divalent cation. This technique revealed high-affinity interactions between the cation and all o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-010
更新日期:1986-01-01 00:00:00
abstract::We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N', N'-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-040
更新日期:2005-08-01 00:00:00
abstract::Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains. The alternation of efficienc...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-177
更新日期:1985-10-01 00:00:00
abstract::Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-063
更新日期:2012-07-01 00:00:00
abstract::A bacteriocin, streptococcin sal-P, was isolated by freeze-thaw elution from cultures of Streptococcus salivarius strain P grown on tryptic soy agar plus 1% neopeptone. The inhibitor could also be extracted with either 7 M urea of 1 M NaCl from cells grown on this medium, but little activity was recovered from cells g...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-144
更新日期:1981-09-01 00:00:00
abstract::The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-022
更新日期:1978-02-01 00:00:00
abstract::Fungal cultures were grown by a new microslide method and by the traditional slide-culture method. Sporulation results were similar by both methods. The microslide method has the advantages of increased safety to the user and ease of storage. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-204
更新日期:1978-10-01 00:00:00
abstract::The results of sucrose-density-gradient centrifugation of a cell-free particulate enzyme system from Acetobacter xylinum which was incubated with uridine diphosphoglucose indicate that there is a polymeric intermediate in the biosynthesis of cellulose. This intermediate has the properties of an oligomer of glucose, is...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-018
更新日期:1975-02-01 00:00:00
abstract::Absorption of 14C-labelled poliovirus-2 to sedimentable solids of primary sludge samples collected from a secondary treatment facility during a 6-month period averaged 94%; for anaerobically digested sludge, 99%. The extent of virus adsorption was influenced by the amount of solids. Maximal adsorption occurred at or a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-254
更新日期:1983-12-01 00:00:00
abstract::Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0464
更新日期:2019-06-01 00:00:00
abstract::Aspergillus caespitosus NRRL 5769 growing in broth containing small amounts of sitosterol produced substance(s) with greater inhibitory activity against Candida albicans ATCC 10231 than in broth without sitosterol. Subsequent isolation, purification, and structural elucidation yielded 5,6-dihydro-5(S)-acetoxy-6(S)-1,2...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-259
更新日期:1975-11-01 00:00:00
abstract::Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2017-0221
更新日期:2017-09-01 00:00:00
abstract::To facilitate the recognition of plant viruses with similar characteristics a dendrogram of characterized viruses was constructed. The sequence of criteria included: type of nucleic acid; single or double stranded; presence or absence of lipid envelope; helical or nonhelical symmetry; and divided or single genome. Non...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-197
更新日期:1977-09-01 00:00:00
abstract::The role the environment has on populations of Pythium and Mesocriconema spp. was investigated at 5 golf course locations in east central Alabama. Every 4 to 5 weeks soil samples were collected from 3 golf greens on each of the 5 golf courses. Environmental data, including air and soil temperature, pH and relative hum...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-005
更新日期:2005-04-01 00:00:00
abstract::Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2020-0239
更新日期:2021-01-01 00:00:00
abstract::Two strains of Bacillus sphaericus. SSII-1 and 1593, were bioassayed for toxic activity against second-instar larvae of the mosquito Culex pipiens quinquefasciatus. It was found that strain 1593 developed a level of toxicity 3000 times that of strain SSII-1. Although the toxic activity of B. sphaericus SSII-1 was rela...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-193
更新日期:1979-11-01 00:00:00
abstract::Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-016
更新日期:1994-02-01 00:00:00
abstract::Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-083
更新日期:1979-05-01 00:00:00
abstract::beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-146
更新日期:2009-04-01 00:00:00
abstract::A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-151
更新日期:1977-08-01 00:00:00
abstract::The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investig...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-103
更新日期:2011-02-01 00:00:00
abstract::The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-105
更新日期:2007-12-01 00:00:00