Abstract:
:Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with cells grown in common laboratory media. This sialic acid was removed by incubation with neuraminidase, showing that it was on the bacterial surface. Serum-dependent sialylation was dependent on metabolic activity, as the addition of chloramphenicol reduced the amount of added sialic acid by more than 90%. Probing the cell surface with an antibody specific for group B streptococcus type III capsular sialic acid showed an increase in antibody binding after growth in human serum. This effect could be lowered by incubating serum-grown cells in neuraminidase prior to antibody exposure. A group B streptococcus mutant that when grown in laboratory media lacks cell surface sialic acid showed significant cell surface sialic acid when grown in human serum. This increase was associated with a significantly decreased ability to bind C3 and hence activate the alternative complement pathway.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Platt MW,Correa N Jr,Mold Cdoi
10.1139/m94-016subject
Has Abstractpub_date
1994-02-01 00:00:00pages
99-105issue
2eissn
0008-4166issn
1480-3275journal_volume
40pub_type
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-083
更新日期:2005-11-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:1975-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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abstract::Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Canadian journal of microbiology
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abstract::Urease activity was measured using whole cells of both long (swarming) and short (nonswarming) populations of Proteus mirabilis from casein hydrolysate agar (CHA) and broth (CHB) cultures, and from brain heart infusion broth (BHIB) cultures. Urease is a constitutive enzyme for both long and short cells, but its activi...
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journal_title:Canadian journal of microbiology
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doi:
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abstract::Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipi...
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pub_type: 杂志文章
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更新日期:1990-06-01 00:00:00
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更新日期:2019-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
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pub_type: 杂志文章
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abstract::The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-174
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1994-10-01 00:00:00
abstract::The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00