Abstract：
:Caulobacters are biofilm-forming members of the natural flora of soil and aquatic environments, which exhibit several characteristics that make them attractive for development of high surface area microbial bioreactors or biosensors. Although caulobacters are well characterized genetically, little is known about their biofilm-forming characteristics as a monoculture, or their tolerance of bioreactor-like conditions. Here we investigated the ability of caulobacters to spontaneously form high-density monolayers on artificial surfaces under a variety of environmental conditions, using phase contrast image analysis to assess biofilm density, and epifluorescence with the vital stain DiBAC to assess viability. With adequate nutrition, extremely dense monolayers formed within 24-48 h, and maintained near 100% viability in experiments ranging up to 22 days. When areas were abraded to remove cells, repopulation occurred rapidly with characteristics similar to the population of a clean surface. When established monolayers were starved for nutrients, a significant fraction of the cells detached from the surface, and cells remaining on the surface no longer tested as viable. Within 4-6 h of nutrient restoration, however, cells in the monolayer again appeared normal and tested as 100% viable. This is the first demonstration that Caulobacter crescentus is stable and amenable to high density monolayer growth and resists starvation, though some cells may express a programmed response to detach from the surface under severe nutrient limitation.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Smit J,Sherwood CS,Turner RFsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
339-49issue
4eissn
0008-4166issn
1480-3275journal_volume
46pub_type
杂志文章abstract：:In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agri...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-123
更新日期：2009-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-232
更新日期：1984-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w07-115
更新日期：2008-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-061
更新日期：2000-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-164
更新日期：1980-08-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-094
更新日期：1978-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-004
更新日期：1986-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-062
更新日期：2002-08-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-111
更新日期：2000-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-188
更新日期：1979-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-107
更新日期：1988-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-180
更新日期：1979-10-01 00:00:00
abstract：:Increasing the use of nitrogen fertilizers in tea orchards has led to intense nitrous oxide (N2O) emissions. Foliar application of Paenibacillus polymyxa biofertilizer has been proven to be beneficial for organic tea production. In this study, tea yield and quality were significantly improved after application of P. p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0511
更新日期：2020-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-106
更新日期：1988-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-042
更新日期：1977-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-065
更新日期：1984-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0630
更新日期：2015-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Several strains of Rhizobium japonicum have been reported to consist of mixtures of stable derivativess having distinct colony morphologies and physiological characteristics. We isolated derivatives from strains of R. japonicum and systematically compared them with previously isolated derivatives with respect to the u...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-106
更新日期：1980-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-174
更新日期：1976-08-01 00:00:00
abstract：:A large pool of antibiotic resistant and auxotrophic mutants was isolated from the Staphylococcus aureus phage group 2 strains UT0002-19 and UT0017 by (1) antibiotic gradient plates, (2) trimethoprim selection, and (3) nitrosoguanidine mutagenesis, which sometimes was coupled by enrichment with either penicillin or me...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-161
更新日期：1980-08-01 00:00:00
abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m95-091
更新日期：1995-08-01 00:00:00
abstract：:Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both st...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-195
更新日期：1984-10-01 00:00:00
abstract：:In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-186
更新日期：1988-09-01 00:00:00
abstract：:A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydosp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-093
更新日期：1975-05-01 00:00:00
abstract：:The effects of the Clostridium difficile toxin were examined in HeLa and mouse adrenal tumor (MAT) cells. Cytotoxicity was evaluated by vital dye exclusion and 51Cr release. In both HeLa and MAT cells, C. difficile toxin caused rounding of virtually 100% of cells. This rounding was distinguishable from rounding produc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-009
更新日期：1982-01-01 00:00:00
abstract：:The results of sucrose-density-gradient centrifugation of a cell-free particulate enzyme system from Acetobacter xylinum which was incubated with uridine diphosphoglucose indicate that there is a polymeric intermediate in the biosynthesis of cellulose. This intermediate has the properties of an oligomer of glucose, is...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-018
更新日期：1975-02-01 00:00:00
abstract：:To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a me...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-096
更新日期：2005-12-01 00:00:00
