Abstract：
:The phyllosphere, which supports a large number of microorganisms, represents the interface between the aboveground parts of plants and air. In this study, four nifH clone libraries were constructed from the phyllosphere of Pyrus serotina (L), Vitis vinifera (P), Prunus armeniaca (X), and Prunus avium (Y). Clones related to Skermanella (L, 12.1%; X, 15.6%; Y, 62.5%; P 70.8%), Bradyrhizobium (X, 2.1%; P, 15.1%; L, 63.7%), Erwinia (X, 68.8%), Pseudomonas (L, 3.3%; P, 7.6%), and Chroococcidiopsis (P, 0.9%; L, 4.4%, X; 5.2%, Y; 19.6%) were present at high percentages, highlighting their critical role in contributing nitrogen to the phyllosphere ecosystem. The 16S rDNA sequence analysis suggested that phyllosphere-associated bacteria were affiliated with a wide range of taxa, encompassing members from Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Cyanobacteria, Tenericutes, and Deinococcus-Thermus. Additionally, the abundance of the nifH gene and 16S rDNA was assessed with quantitative PCR. The number of copies of nifH and 16S rDNA ranged from 1.14 × 103 to 1.49 × 104 and from 3.72 × 106 to 7.02 × 107 copies/g fresh leaf sample, respectively. In conclusion, our work sheds light on the microbial communities of the phyllosphere that are important for plant growth. Moreover, we observed a unique composition of nitrogen-fixing bacteria in each phyllosphere sample, suggesting the existence of specific interactions between these functional microorganism and plants, which may provide information or be a reference for the development of bacterial fertilizers.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Liang S,Liu H,Wu S,Xu S,Jin D,Faiola F,Zhuang X,Zhuang G,Qu D,Fan H,Bai Zdoi
10.1139/cjm-2018-0588subject
Has Abstractpub_date
2019-09-01 00:00:00pages
642-652issue
9eissn
0008-4166issn
1480-3275journal_volume
65pub_type
杂志文章abstract：:A strain of Pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. A CoA-mediated beta-oxidation pathway was induced for the metabolism of the compound. The organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-198
更新日期：1982-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0481
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-077
更新日期：1989-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-187
更新日期：1982-11-01 00:00:00
abstract：:Photorhabdus temperata, an insect pathogen and nematode symbiont, is motile in liquid medium by swimming. We found that P. temperata was capable of surface movement, termed swarming behavior. Several lines of evidence indicate that P. temperata use the same flagella for both swimming and swarming motility. Both motili...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W10-119
更新日期：2011-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-122
更新日期：1994-09-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/w11-131
更新日期：2012-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w98-225
更新日期：1999-02-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/m75-251
更新日期：1975-11-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-015
更新日期：1978-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-162
更新日期：1985-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-175
更新日期：1989-11-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-099
更新日期：1991-08-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-078
更新日期：1981-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-049
更新日期：1986-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:Five strains of Haemophilus pleuropneumoniae, out of eight strains tested, produced extracellular haemolysin(s) when grown in liquid culture in the presence, but not in the absence, of RNA. The haemolysin produced by the neotype strain was unstable, heat labile, and sensitive to degradation by pronase, trypsin, and ch...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-085
更新日期：1985-05-01 00:00:00
abstract：:The shikimate metabolites are key precursors to a large number of natural products, including aromatic amino acids. Chorismic acid is an important branch point in the biosynthetic pathway to aromatic amino acids. Chorismic acid is also unique among natural products since it is the only compound known to undergo an enz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-043
更新日期：1991-04-01 00:00:00
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-157
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journal_title：Canadian journal of microbiology
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doi：10.1139/m77-191
更新日期：1977-09-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/cjm-2013-0357
更新日期：2013-10-01 00:00:00
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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doi：10.1139/W10-087
更新日期：2010-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-049
更新日期：1983-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：1989-07-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-257
更新日期：1983-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-115
更新日期：1976-06-01 00:00:00
