Glutaraldehyde reactions with alkaline phosphatase of Pseudomonas aeruginosa.

Abstract:

:Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified protein was compared with whole, cell-bound enzyme. The effects of the reagent on the kinetics of the purified enzyme were studied and some conclusions drawn as to the mode of inactivation. The reaction of glutaraldehyde with the cell envelope of P. aeruginosa was also investigated, and it was found not to modify the extraction of lipopolysaccharides from the outer membrane. This study emphasizes the care that must be taken to interpret data, cytochemical or otherwise, obtained when glutaraldehyde is used as a fixative or cross-linking reagent.

journal_name

Can J Microbiol

authors

Day DF,Ingram JM

doi

10.1139/m81-078

subject

Has Abstract

pub_date

1981-05-01 00:00:00

pages

531-5

issue

5

eissn

0008-4166

issn

1480-3275

journal_volume

27

pub_type

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