Oxylipin and mitochondrion probes to track yeast sexual cells.

Abstract:

:When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests increased mitochondrial activity in asci, since 3-OH oxylipins are mitochondrially produced and it is known that Rh123 accumulates selectively in functional mitochondria that maintain a high transmembrane potential (Delta Psi m). This increased activity may be necessary for the production and effective release of the many spores found in single-celled asci. These results may be useful in the rapid identification of asci and in yeast sexual spore mechanics, which may find application in yeast systematics as well as hydro-, aero-, and nano-technologies.

journal_name

Can J Microbiol

authors

Ncango DM,Swart CW,Goldblatt ME,Pohl CH,Van Wyk PW,Botes PJ,Kock JL

doi

10.1139/W08-035

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

450-5

issue

6

eissn

0008-4166

issn

1480-3275

pii

w08-035

journal_volume

54

pub_type

杂志文章
  • In vivo associations of Escherichia coli NarJ with a peptide of the first 50 residues of nitrate reductase catalytic subunit NarG.

    abstract::The catalytic subunit of many Escherichia coli redox enzymes bares a twin-arginine translocation (Tat)-dependent signal peptide in its precursor, which directs the redox enzyme complex to this Sec-independent pathway. NarG of the E. coli nitrate reductase NarGHI complex possesses a vestige twin-arginine motif at its N...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-111

    authors: Li H,Turner RJ

    更新日期:2009-02-01 00:00:00

  • Potential of the volatile-producing fungus Muscodor albus for control of building molds.

    abstract::The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W06-124

    authors: Mercier J,Jiménez JI

    更新日期:2007-03-01 00:00:00

  • Suitability of membrane-filter techniques to study the ultrastructure of Fusarium solani in soil.

    abstract::The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-239

    authors: Van Eck WH

    更新日期:1976-11-01 00:00:00

  • Thymineless death in Escherichia coli mutants deficient in the RecF recombination pathway.

    abstract::Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-157

    authors: Nakayama K,Shiota S,Nakayama H

    更新日期:1988-07-01 00:00:00

  • Chemical and serological properties of a cyanogen bromide peptide of southern bean mosaic virus protein.

    abstract::Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-241

    authors: Tremaine JH,Ronald WP,Kelly EM

    更新日期:1980-12-01 00:00:00

  • Cell-free, protein-synthesizing system of photosynthetic and heterotrophic Rhodospirillum rubrum.

    abstract::An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-042

    authors: Chow CT

    更新日期:1976-02-01 00:00:00

  • The presence of 3-hydroxy oxylipins on the ascospore surfaces of some species representing Saccharomycopsis Schiönning.

    abstract::Through gas chromatography - mass spectrometry, the presence of oxylipins, mainly 3-hydroxy 9:1 and 3-hydroxy 10:1, was detected in Saccharomycopsis fermentans, Saccharomycopsis javanensis, and Saccharomycopsis vini. The distribution of these compounds was mapped using immunofluorescence microscopy, and they were foun...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-037

    authors: Sebolai OM,Kock JL,Pohl CH,Botes PJ,Strauss CJ,Van Wyk PW,Nigam S

    更新日期:2005-07-01 00:00:00

  • Alkaline phosphatase and a cellulase reporter protein are not exported from the cytoplasm when fused to large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein.

    abstract::Using a gene fusion approach, hybrid proteins were created by linking alkaline phosphatase (PhoA) or a cellulase reporter (delta CenA) to four large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein (RsaA; 1026 amino acids). Three of the sites (amino acids 189, 220, 315) were selected on the ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m94-122

    authors: Bingle WH,Smit J

    更新日期:1994-09-01 00:00:00

  • Performance of ectomycorrhizal alders exposed to specific Canadian oil sands tailing stressors under in vivo bipartite symbiotic conditions.

    abstract::Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0703

    authors: Beaudoin-Nadeau M,Gagné A,Bissonnette C,Bélanger PA,Fortin JA,Roy S,Greer CW,Khasa DP

    更新日期:2016-07-01 00:00:00

  • Immunosuppression, nonspecific B-cell activation, and mitogenic activity associated with a high molecular weight component from Listeria monocytogenes.

    abstract::A high molecular component of a saline extract derived from Listeria monocytogenes contained amino acids, carbohydrates, and phosphorus. The same fraction was capable of promoting both the in vitro mitogenic and adjuvant activities and the in vivo immunosuppressive activity displayed by the crude extract. The material...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-204

    authors: Otokunefor TV,Galsworthy SB

    更新日期:1982-12-01 00:00:00

  • Comparison of thermostable macromolecular antigens from leprosy-associated bacteria.

    abstract::Three types of bacteria are associated with leprosy: Mycobacterium leprae, leprosy-derived corynebacteria (LDC), and armadillo-derived mycobacteria (ADM). The immunological relationships between these three types of bacteria and Mycobacterium bovis BCG, used as a reference, were determined by cross-immunoelectrophores...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m94-083

    authors: Baelden MC,Bouckaert AE,Grégoire D,van de Poele M,Coene M

    更新日期:1994-06-01 00:00:00

  • Neutralizing response of rabbits to an experimental rubella subunit vaccine made from immunostimulating complexes.

    abstract::The purpose of this study was to evaluate experimentally the immunogenicity in rabbits of rubella subunits adsorbed to the adjuvant Quil A. The adsorbed viral proteins form structurally defined ImmunoStimulating COMplexes (ISCOMs). Rubella ISCOMs were tested for their capacity to induce neutralizing and hemagglutinati...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-238

    authors: Trudel M,Nadon F,Séguin C,Payment P

    更新日期:1988-12-01 00:00:00

  • Polygalacturonase isolated from the culture of the psychrophilic fungus Sclerotinia borealis.

    abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m97-059

    authors: Takasawa T,Sagisaka K,Yagi K,Uchiyama K,Aoki A,Takaoka K,Yamamato K

    更新日期:1997-05-01 00:00:00

  • Genomic comparison of facultatively anaerobic and obligatory aerobic Caldibacillus debilis strains GB1 and Tf helps explain physiological differences.

    abstract::Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0464

    authors: Wushke S,Froese A,Fristensky B,Zhang XL,Spicer V,Krokhin OV,Levin DB,Sparling R

    更新日期:2019-06-01 00:00:00

  • Effects of Gypsophila saponins on bacterial growth kinetics and on selection of subterranean clover rhizosphere bacteria.

    abstract::Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-052

    authors: Fons F,Amellal N,Leyval C,Saint-Martin N,Henry M

    更新日期:2003-06-01 00:00:00

  • Streptococci and lactococci synthesize large amounts of HPr(Ser-P)(His~P).

    abstract::HPr is a protein of the phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In gram-positive bacteria, HPr can be phosphorylated on Ser-46 by the kinase/phosphorylase HprK/P and on His-15 by phospho-enzyme I (EI~P) of the PTS. In vitro studies with purified HPrs from Bacillus subtilis, Enterococcus fa...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-085

    authors: Roy DJ,Casabon I,Vaillancourt K,Huot JL,Vadeboncoeur C

    更新日期:2008-11-01 00:00:00

  • A novel approach for the improvement of ethanol fermentation by Saccharomyces cerevisiae.

    abstract::Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involv...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-032

    authors: Hou L,Cao X,Wang C

    更新日期:2010-06-01 00:00:00

  • The fine structure of conidial development in the genus Torula. II. T. caligans (Batista and Upadhyay) M. B. Ellis and T. terrestris Mistra.

    abstract::Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-279

    authors: Ellis DH,Griffiths DA

    更新日期:1975-12-01 00:00:00

  • Preliminary report on the observation of a coronavirus in the intestine of the laboratory rabbit.

    abstract::Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-201

    authors: Lapierre J,Marsolais G,Pilon P,Descôteaux JP

    更新日期:1980-10-01 00:00:00

  • Fungal fimbriae. I. Structure, origin, and synthesis.

    abstract::Fine hair-like appendages on the cell walls of the another smut Ustilago violacea are described. These hairs are termed fimbriae because of their close similarity to the fimbriae (pili) found on certain Gram-negative bacteria. Cells of U. violacea may carry more than 200 fimbriae varying in length from about 0.5 mum t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-076

    authors: Poon NH,Day AW

    更新日期:1975-04-01 00:00:00

  • Biosynthesis of isochorismate in Klebsiella pneumoniae: origin of O-2.

    abstract::The shikimate metabolites are key precursors to a large number of natural products, including aromatic amino acids. Chorismic acid is an important branch point in the biosynthetic pathway to aromatic amino acids. Chorismic acid is also unique among natural products since it is the only compound known to undergo an enz...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m91-043

    authors: Zamir LO,Devor KA,Jensen RA,Tiberio R,Sauriol F,Mamer O

    更新日期:1991-04-01 00:00:00

  • Binding of the Bacillus sphaericus mosquito larvicidal toxin to cultured insect cells.

    abstract::Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m87-173

    authors: Davidson EW,Shellabarger C,Meyer M,Bieber AL

    更新日期:1987-11-01 00:00:00

  • Intrafungal distribution of aflatoxins among conidia and sclerotia of Aspergillus flavus and Aspergillus parasiticus.

    abstract::This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-001

    authors: Wicklow DT,Shotwell OL

    更新日期:1983-01-01 00:00:00

  • Antifungal susceptibility and genotypical pattern of Microsporum canis strains.

    abstract::Dermatophytes are a group of fungi that are capable of invading keratinized tissues of humans and other animals. Antifungal susceptibility analysis and genetic studies by random amplification of polymorphic DNA (RAPD), have been used to detect polymorphism as well as determining the possible resistance of dermatophyte...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-024

    authors: Brilhante RS,Cordeiro RA,Medrano DJ,Monteiro AJ,Sidrim JJ,Rocha MF

    更新日期:2005-06-01 00:00:00

  • [Presence of Pseudomonas maltophilia in the rhizosphere of several cultivated plants].

    abstract::Pseudomonas maltophilia has been identified in the rhizosphere of several cultured plants: cabbage, rape, mustard, corn, beet. In comparison with nonrhizospheric soil, all the rhizospheric samples analysed contain more of this Pseudomonas and the most important stimulation is supplied by cruciferous plants. The possib...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Debette J,Blondeau R

    更新日期:1980-04-01 00:00:00

  • Growth of group B streptococci in human serum leads to increased cell surface sialic acid and decreased activation of the alternative complement pathway.

    abstract::Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m94-016

    authors: Platt MW,Correa N Jr,Mold C

    更新日期:1994-02-01 00:00:00

  • Immunovirological studies on human respiratory syncytial virus structural proteins.

    abstract::Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-004

    authors: Trudel M,Nadon F,Séguin C,Ghoubril S,Payment P,Trépanier P

    更新日期:1986-01-01 00:00:00

  • Staphylococcus aureus strains differ in their in vitro responsiveness to human urokinase: evidence that methicillin-resistant strains are predominately nonresponsive to the growth-enhancing effects of urokinase.

    abstract::Clinical isolates of Staphylococcus aureus were found to exhibit strain-specific heterogeneity to the growth-enhancing effects of human urokinase (UK), a proteinase with plasminogen activator activity. Nine out of fourteen (64%) methicillin-sensitive strains of S. aureus were responsive to UK in "in vitro" cultures. I...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-131

    authors: Hart DA,Louie T,Krulicki W,Reno C

    更新日期:1996-10-01 00:00:00

  • Biological control of fusarial wilt of pigeon pea by Bacillus brevis.

    abstract::A virulent strain of pigeon pea wilt pathogen was isolated from wilted pigeon pea plants and was identified as Fusarium oxysporum f. sp. udum. Many bacterial cultures showing antagonism to the pathogen were isolated from various ecological niches. When tested under pot and field conditions, development of fusarial wil...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w99-109

    authors: Bapat S,Shah AK

    更新日期:2000-02-01 00:00:00

  • Proteolytic activity of Oidiodendron kalrai.

    abstract::The physiochemical characteristics of the intracellular proteolytic enzymes of Oidiodendron kalari, a neuropathogenic fungus, were studied. The organism in the yeast phase was grown in a semisynthetic medium containing 1% tryptone, at 37 degrees C for 48 hr, on a gyrotory shaker. The crude extract was prepared by brea...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-204

    authors: Cino PM,Tewari RP

    更新日期:1975-09-01 00:00:00