Abstract:
:When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests increased mitochondrial activity in asci, since 3-OH oxylipins are mitochondrially produced and it is known that Rh123 accumulates selectively in functional mitochondria that maintain a high transmembrane potential (Delta Psi m). This increased activity may be necessary for the production and effective release of the many spores found in single-celled asci. These results may be useful in the rapid identification of asci and in yeast sexual spore mechanics, which may find application in yeast systematics as well as hydro-, aero-, and nano-technologies.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ncango DM,Swart CW,Goldblatt ME,Pohl CH,Van Wyk PW,Botes PJ,Kock JLdoi
10.1139/W08-035subject
Has Abstractpub_date
2008-06-01 00:00:00pages
450-5issue
6eissn
0008-4166issn
1480-3275pii
w08-035journal_volume
54pub_type
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