Abstract：
:Three types of bacteria are associated with leprosy: Mycobacterium leprae, leprosy-derived corynebacteria (LDC), and armadillo-derived mycobacteria (ADM). The immunological relationships between these three types of bacteria and Mycobacterium bovis BCG, used as a reference, were determined by cross-immunoelectrophoresis. When compared with the reference, cross-reactions were observed with a variable number of antigens: 2 in the case of strain LDC 15, 4 with M. leprae, and from 1 to 10 in the case of the ADM, depending on their subgroup. Next, thermostable macromolecular antigens (TMAs), the major cross-reactive antigens of leprosy-associated bacteria, were compared by anti-TMA antibody ELISA tests. The LDC TMAs displayed high cross-reactivity between the subgroups and lower cross-reactivity with the TMAs of M. bovis BCG. Evidence for the presence of a species-specific moiety in TMA of the different LDC was obtained by using depleted anti-TMA antisera. Western blot analysis revealed the presence of many proteins in the TMAs of LDC and M. bovis BCG, some of them being species-specific and other cross-reactive.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Baelden MC,Bouckaert AE,Grégoire D,van de Poele M,Coene Mdoi
10.1139/m94-083subject
Has Abstractpub_date
1994-06-01 00:00:00pages
508-12issue
6eissn
0008-4166issn
1480-3275journal_volume
40pub_type
杂志文章abstract：:The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-057
更新日期：1994-05-01 00:00:00
abstract：:The release of 14CO2 from 14C-labelled glucose(G-1(-14)C, G-3,4(-14)C, G-6(-14)C) was followed in phased cultures of Candida utilis grown in a glucose- mineral salts medium under altered conditions of carbon:nitrogen limitation at doubling times of 2,4 and 6, h. Changes in oxygen uptake and CO2 evolution were observe...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-150
更新日期：1975-07-01 00:00:00
abstract：:Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-119
更新日期：2005-02-01 00:00:00
abstract：:A soluble cytochrome P450 (P450EP1A) induced by 2-ethoxyphenol was purified to apparent homogeneity from Corynebacterium sp. strain EP1. The P450EP1A showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of about 45 kDa. The CO-reduced difference spectra of P450EP1A ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-177
更新日期：1983-09-01 00:00:00
abstract：:Staphylococcus aureus was grown in a rich peptone medium which became alkaline with continued incubation. Cells were grown at 37 degrees C and in the same medium containing 1 M NaCl at 46 degrees C, a temperature at which this organism can grow only when protected by NaCl. Cells of these cultures are hereafter called ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-049
更新日期：1983-03-01 00:00:00
abstract：:The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-077
更新日期：2007-09-01 00:00:00
abstract：:Bifidobacteria are generally acknowledged as major gut microflora used as probiotics, which promote human health. In this study, the effects of the administration of Bifidobacterim bifidum on modulating gastrointestinal (GI) tract microflora, acid and bile resistance, and physiological indices in BALB/c mice were inve...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0694
更新日期：2015-02-01 00:00:00
abstract：:The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m88-093
更新日期：1988-04-01 00:00:00
abstract：:An obligate methyltroph Methylomonas methylovora oxidized methylamine, formaldehyde, and formate. Enzymes oxidizing these substrates were detected in a cell-free system. Phenazine methosulfate-linked methylamine dehydrogenase was purified 21-fold. The enzyme had optimum activity at pH 7.5 and was stable at 60 degrees ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-059
更新日期：1977-04-01 00:00:00
abstract：:Ninety-five fecal samples from Atlantic cod (Gadus morhua L.), caught along the northern Norwegian coast, were examined bacteriologically for occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (alpha, beta, epsilon, and iota) for classifica...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-015
更新日期：2002-04-01 00:00:00
abstract：:Vero cells were more suitable then BHK-21 cells for the detection of rubella-specific IgG antibodies because the nonspecific fluorescence was minimal. However, BHK-21 cells were found more sensitive than Vero cells for the detection of rubella-specific IgM antibodies. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-041
更新日期：1975-03-01 00:00:00
abstract：:Two bacterial species, Gram-negative Escherichia coli and Gram-positive Bacillus subtilis, were exposed to different auxins to examine possible effects of these substances on bacterial stress tolerance. Bacterial resistance to UV irradiation, heat shock, and streptomycin was assessed with and without previous exposure...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0266
更新日期：2013-11-01 00:00:00
abstract：:The present investigation was aimed to scrutinize the salt tolerance potential of plant-growth-promoting rhizobacteria (PGPR) isolated from rhizospheric soil of selected halophytes (Atriplex leucoclada, Haloxylon salicornicum, Lespedeza bicolor, Suaeda fruticosa, and Salicornica virginica) collected from high-saline f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0668
更新日期：2015-04-01 00:00:00
abstract：:Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-065
更新日期：1976-03-01 00:00:00
abstract：:The role the environment has on populations of Pythium and Mesocriconema spp. was investigated at 5 golf course locations in east central Alabama. Every 4 to 5 weeks soil samples were collected from 3 golf greens on each of the 5 golf courses. Environmental data, including air and soil temperature, pH and relative hum...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-005
更新日期：2005-04-01 00:00:00
abstract：:The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-027
更新日期：1988-02-01 00:00:00
abstract：:Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-083
更新日期：2005-11-01 00:00:00
abstract：:In this work, we assayed the ability of newly prepared indolizine derivates (epimers) 6C and 6A to inhibit the growth of Mycobacterium smegmatis and used them for resistance induction. 6A inhibited the growth of M. smegmatis at a concentration of 100 μg/mL. No inhibitory effect was observed in the presence of 6C. By i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0438
更新日期：2013-02-01 00:00:00
abstract：:Wild-type glucose uptake and glucose chemotaxis activities were restored in glucose transport defective Pseudomonas aeruginosa strains PFB360 and PFB362 after introduction of plasmid pPZ129, containing a 1.1-kilobase DNA fragment that is essential for the expression of the P. aeruginosa periplasmic glucose binding pro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-163
更新日期：1993-11-01 00:00:00
abstract：:Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-024
更新日期：1980-02-01 00:00:00
abstract：:Formate dehydrogenase activity (EC 1.2.1.2) has been demonstrated in cell-free preparations of Mycobacterium phlei by following the reduction of 2,6 dichlorophenolindophenol. thiazolyl blue tetrazolium, or equine cytochrome c. The reduction of equine cytochrome c was inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide. N...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-018
更新日期：1977-02-01 00:00:00
abstract：:Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0239
更新日期：2021-01-01 00:00:00
abstract：:Seven tk- mutants of herpes simplex virus, type 2 (HSV-2), and three tk- mutants of herpes simplex virus, type 1 (HSV-1), were isolated which did not produce the thymidine kinase (TK) polypeptides but formed smaller polypeptides not seen in wild-type infected cells. Positive TK mRNA selection by hybridization to the c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-063
更新日期：1983-04-01 00:00:00
abstract：:Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-021
更新日期：1989-01-01 00:00:00
abstract：:Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0399
更新日期：2014-10-01 00:00:00
abstract：:Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-046
更新日期：2001-06-01 00:00:00
abstract：:Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial ce...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-114
更新日期：1989-07-01 00:00:00
abstract：:Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
