Abstract:
:The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells than to G0-G1 phase cells. Furthermore, bacterial binding curves exhibited a plateau of binding to G2 cells at a normalized bacteria to cell ratio approximately 1.8 times larger than that at which the plateau of binding to G0-G1 cell was observed. Since G2 cells are on average 1.4-1.8 times larger than G0-G1 cells, the results indicate that bacterial binding is proportional to cell size and not to the preferential binding (higher affinity) of bacteria to A549 cells in the G2 phase. Finally, the non-Gaussian distribution of bacterial binding could be mathematically modeled by a linear combination of three Gaussian distributions each representing bacterial binding to cells in a particular phase of the cell cycle (G0-G1, S, and G2-M). Because the Gaussian function contains a term that takes into account the relative number of cells in each of the phases, this last result implies that the overall (non-Gaussian) binding distribution (and hence the median of bacterial binding) can be highly sensitive to the relative proportion of cells in the various phases of the cell cycle.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Bérubé LR,Jouishomme H,Jarrell HCsubject
Has Abstractpub_date
1998-05-01 00:00:00pages
448-55issue
5eissn
0008-4166issn
1480-3275journal_volume
44pub_type
杂志文章abstract::The nucleotide sequence of the murE gene encoding the diaminopimelic acid adding enzyme of Escherichia coli is reported. The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs. The deduced primary structure of MurE comprised 471 amino acid re...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-175
更新日期:1989-11-01 00:00:00
abstract::A previously unmapped pan gene was localized in an Escherichia coli K-12 strain using Hfr and F' matings and by transduction studies. This Pan- mutation was shown by supplementation studies to impart a block in pantoate biosynthesis. The pan gene cotransduced with rha, metB, and argE placing it at 87 min. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-190
更新日期:1981-11-01 00:00:00
abstract::The regulation of de novo pyrimidine biosynthesis in Pseudomonas putida ATCC 17536 by pyrimidines was explored. The pathway enzyme activities were higher in glucose-grown cells than in succinate-grown cells, indicating catabolite repression by succinate. In P. putida cells grown on succinate as a carbon source, only a...
journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w98-224
更新日期:1999-03-01 00:00:00
abstract::Bacterial biofilms are commonly formed on medical devices and food processing surfaces. The antimicrobials used have limited efficacy against the biofilms; therefore, new strategies to prevent and remove these structures are needed. Here, the effectiveness of brief oxidative treatments, based on the combination of sod...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0747
更新日期:2015-05-01 00:00:00
abstract::The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-100
更新日期:1989-06-01 00:00:00
abstract::Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-052
更新日期:2003-06-01 00:00:00
abstract::Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0206
更新日期:2014-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-135
更新日期:2006-04-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:10.1139/w10-047
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0528
更新日期:2015-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-141
更新日期:1976-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-032
更新日期:1976-02-01 00:00:00
abstract::The marine sponge Mycale sp., a potential source of natural bioactive products, is widely distributed along the coast of Fujian, China. The cultivable bacterial community associated with Mycale sp., the antibacterial activities, and the PKS (polyketide synthase) and NRPS (nonribosomal peptide synthetase) gene diversit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0785
更新日期:2014-04-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:10.1139/w04-026
更新日期:2004-06-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0485
更新日期:2014-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-153
更新日期:1978-08-01 00:00:00
abstract::The binding of mutagenic pyrolyzates to cell fractions from some gram-negative intestinal bacteria and to thermally treated bacterial cells was investigated. 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) were effectively bound by several of the bacterial ce...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-101
更新日期:1992-07-01 00:00:00
abstract::In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-188
更新日期:1979-10-01 00:00:00
abstract::Group B Neisseria meningitidis (SD1C) was grown on defined medium supplemented with each of a variety of sulphur compounds as the sole source of sulphur. The organism grew on sulphate, sulphite, bisulphite, thiosulphate, dithionite, hydrosulphide, thiocyanate, L-cysteine, L-cystine, reduced glutathione, methionine, me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-232
更新日期:1984-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-193
更新日期:1979-11-01 00:00:00
abstract::Escherichia coli is capable of producing several virulence factors involved in the pathophysiology of bovine mastitis. Cell surface structures such as flagella, pili, and other outer membrane adhesins are considered essential for biofilm formation on abiotic surfaces. The aim of this study was characterize the molecul...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0053
更新日期:2013-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0412
更新日期:2020-04-01 00:00:00
abstract::The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-032
更新日期:1983-02-01 00:00:00
abstract::Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-073
更新日期:1997-06-01 00:00:00
abstract::Citrobacter freundii from an environmental source displayed resistance to a wide variety of antibacterials. Some of these resistances could be transferred by conjugation with frequencies as high as 10(-2). The complexity of plasmid involvement in the antibacterial resistance of this species was demonstrated by isolati...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-052
更新日期:1981-03-01 00:00:00
abstract::Neisseria polysaccharea (LNP 462, NCTC 11858), proposed as a prototype strain constituting a new taxon in the genus Neisseria, produces copious amounts of polysaccharide when grown on agar containing 1-5% sucrose. Plate-grown cells produced an exocellular polysaccharide which was composed of D-glucose, had [alpha]D +2...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-167
更新日期:1986-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-120
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abstract::Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-093
更新日期:2009-11-01 00:00:00
abstract::Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w98-225
更新日期:1999-02-01 00:00:00