Purification and characterization of 2-ethoxyphenol-induced cytochrome P450 from Corynebacterium sp. strain EP1.

Abstract:

:A soluble cytochrome P450 (P450EP1A) induced by 2-ethoxyphenol was purified to apparent homogeneity from Corynebacterium sp. strain EP1. The P450EP1A showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of about 45 kDa. The CO-reduced difference spectra of P450EP1A had a Soret maximum at 447.6 nm. The substrate difference spectra with 2-ethoxyphenol showed an absorption maximum at 394.0 nm. The purified P450EP1A degraded 2-ethoxyphenol in an assay system composed of spinach ferredoxin-NADP+ oxidoreductase and NADPH. The reaction activity decreased to 1.4% of its original activity by addition of CO. The existence of catechol in the reaction mixture was confirmed after the metabolic reaction, indicating that P450EP1A catalyzes O-dealkylation of 2-ethoxyphenol. In addition to 2-ethoxyphenol, the P450EP1A metabolized 2-methoxyphenol, 1,1,1-trichloroethane, carbon tetrachloride, benzene, and toluene.

journal_name

Can J Microbiol

authors

Kawahara N,Ikatsu H,Kawata H,Miyoshi S,Tomochika K,Sinoda S

subject

Has Abstract

pub_date

1999-10-01 00:00:00

pages

833-9

issue

10

eissn

0008-4166

issn

1480-3275

journal_volume

45

pub_type

杂志文章
  • Complete genome sequence of Paracoccus denitrificans ATCC 19367 and its denitrification characteristics.

    abstract::Studies show that Paracoccus denitrificans can denitrify nitrogen sources under aerobic conditions. However, the lack of data on its genome sequence has restricted molecular studies and practical applications. In this study, the complete genome of P. denitrificans ATCC 19367 was sequenced and its nitrogen metabolism p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2019-0037

    authors: Si YY,Xu KH,Yu XY,Wang MF,Chen XH

    更新日期:2019-07-01 00:00:00

  • Thiamine secretion in yeast.

    abstract::To isolate thiamine excretors and (or) overproducers, 188 cultures belonging to nine yeast and three fungal genera were screened. Nine excreted thiamine as determined by both the presence of cross-feeding zones on a thiamine-free agar medium seeded with a thiamine-requiring yeast strain and by the direct detection of ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-189

    authors: Haj-Ahmad Y,Bilinski CA,Russell I,Stewart GG

    更新日期:1992-11-01 00:00:00

  • Hydrogen peroxide-induced secondary necrosis in conidia of Aspergillus fumigatus.

    abstract::Aspergillus fumigatus conidia have been linked to severe aspergillosis in immunocompromised patients. Recently, the cytotoxic effect of secondary metabolites from A. fumigatus conidia was reported. In the present work, a methodology used to detect cell death markers in fungal hyphae was adapted to study conidia cell d...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0561

    authors: Oliveira M,Pereira C,Bessa C,Araujo R,Saraiva L

    更新日期:2016-02-01 00:00:00

  • Microbes associated with the epithelium of the oesophagus and stomach of guinea pigs (Cavia porcellus).

    abstract::Histological examination of oesophagus and stomach specimens from 28 guinea pigs (Cavia porcellus) revealed microbes associated with the epithelial surface of these organs. Layers of Gram-positive cocci were associated with oesophageal tissue in all the animals. Yeasts (Torulop-sid pintolopesii) were associated with s...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-182

    authors: Yoon CS,Tannock GW

    更新日期:1978-09-01 00:00:00

  • The in vitro hydroxylation of 4'-chloro-4-biphenylol by a mushroom tyrosinase preparation.

    abstract::Incubation of 4'-chloro-4-biphenylol with a mushroom tyrosinase preparation gave the catechol, 4'-chloro-3,4-biphenyldiol as the sole in vitro metabolite. This compound was identical with the major rat urinary metabolite of 4'-chloro-4-biphenylol and thus confirms the structure assigned to the metabolite. This result ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-014

    authors: Safe S,Ellis BE,Hutzinger O

    更新日期:1976-01-01 00:00:00

  • Cell surface properties of two differently virulent strains of Acinetobacter baumannii isolated from a patient.

    abstract::The aim of this study was to unravel, by focusing on cell surface properties, the underlying virulence factors contributing to the difference in the pathogenicity observed in two Acinetobacter baumannii strains isolated from the same patient. The two strains were phenotypically different: (i) a mucoid strain (AB-M),...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-131

    authors: Kempf M,Eveillard M,Deshayes C,Ghamrawi S,Lefrançois C,Georgeault S,Bastiat G,Seifert H,Joly-Guillou ML

    更新日期:2012-03-01 00:00:00

  • Airborne microorganisms in a municipal solid waste recovery system.

    abstract::The types of bacteria and fungi present in the air of a municipal solid waste recovery system have been characterized and the population densities estimated. Conventional methods were successful in enumerating coagulase-positive staphylococci, Klebsiella spp., gram-negative bacteria, fungi, and common indicator organi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m85-038

    authors: Lembke LL,Kniseley RN

    更新日期:1985-03-01 00:00:00

  • Comparison of methods for the concentration of Cryptosporidium oocysts and Giardia cysts from raw waters.

    abstract::This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-059

    authors: Ferguson C,Kaucner C,Krogh M,Deere D,Warnecke M

    更新日期:2004-09-01 00:00:00

  • Extracellular nuclease produced by a marine bacterium. I. Extracellular deoxyribonuclease formation by a marine Vibrio sp.

    abstract::Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these ogranisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. This organism requires salts of seawater for both growth and extracell...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-213

    authors: Maeda M,Taga N

    更新日期:1976-10-01 00:00:00

  • Bacterial endophytes of the wildflower Crocus albiflorus analyzed by characterization of isolates and by a cultivation-independent approach.

    abstract::The presence and taxonomy of endophytic bacteria of the entire aerial parts of crocus (Crocus albiflorus), a wildflower native in the Alps, were investigated. A combination of plating of plant macerates, isolation and sequence identification of isolates, and direct 16S rDNA PCR amplification followed by whole-communit...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-109

    authors: Reiter B,Sessitsch A

    更新日期:2006-02-01 00:00:00

  • The nonrandom binding distribution of Streptococcus pneumoniae to type II pneumocytes in culture is dependent on the relative distribution of cells among the phases of the cell cycle.

    abstract::The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Bérubé LR,Jouishomme H,Jarrell HC

    更新日期:1998-05-01 00:00:00

  • Effect of temperature on growth of guanidine-resistant mutants of poliovirus.

    abstract::Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m85-220

    authors: Tershak DR

    更新日期:1985-12-01 00:00:00

  • Growth of group B streptococci in human serum leads to increased cell surface sialic acid and decreased activation of the alternative complement pathway.

    abstract::Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m94-016

    authors: Platt MW,Correa N Jr,Mold C

    更新日期:1994-02-01 00:00:00

  • The fine structure of conidial development in the genus Torula. II. T. caligans (Batista and Upadhyay) M. B. Ellis and T. terrestris Mistra.

    abstract::Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-279

    authors: Ellis DH,Griffiths DA

    更新日期:1975-12-01 00:00:00

  • Exopolysaccharide depolymerases induced by Rhizobium bacteriophages.

    abstract::Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-239

    authors: Barnet YM,Humphrey B

    更新日期:1975-10-01 00:00:00

  • Hydroxylase regulation in Candida tropicalis grown on alkanes.

    abstract::Candida tropicalis synthesizes a hydroxylase (3 to 5 nmol of product formed per minute per milligram of protein) and a cytochrome P-450 (0.10 to 0.13 nmol per milligram of protein) during growth on n-tetradecane. A three- to four-fold increase in the level of NADPH cytochrome c reductase is also observed in those cell...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-031

    authors: Gilewicz M,Zacek M,Bertrand JC,Azoulay E

    更新日期:1979-02-01 00:00:00

  • Chromosomal bacterial type II toxin-antitoxin systems.

    abstract::Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章,评审

    doi:10.1139/w2012-025

    authors: Syed MA,Lévesque CM

    更新日期:2012-05-01 00:00:00

  • Rv3351c, a Mycobacterium tuberculosis gene that affects bacterial growth and alveolar epithelial cell viability.

    abstract::Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0528

    authors: Pavlicek RL,Fine-Coulson K,Gupta T,Quinn FD,Posey JE,Willby M,Castro-Garza J,Karls RK

    更新日期:2015-12-01 00:00:00

  • DNA base composition, nature of intracellular DNA, morphology, and classification of bacteriophages infecting Micrococcus luteus.

    abstract::Ten bacteriophages infecting Micrococcus luteus have been characterized. All phages contain double-stranded DNA, of 64.3--73.5 mol% guanine plus cytosine (GC). The DNA of phage N7 has the highest GC content reported for any bacterial virus. No unusual bases have been found. The intracellular replicating DNAs of six ph...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-158

    authors: Compton SW,Mayo JA,Ehrlich M,Ackermann HW,Tremblay L,Cords CE,Scaletti JV

    更新日期:1979-09-01 00:00:00

  • Altitudinal distribution of microfungi associated with Betula ermanii leaf litter on Mt. Rishiri, northern Japan.

    abstract::The altitudinal distribution of microfungi associated with Betula ermanii Cham. leaf litter was investigated on Mt. Rishiri (1719 m), northern Japan. Fallen leaves were collected at five sites of different altitudes (approximately 300, 600, 900, 1200, and 1500 m above sea level) along a transect on the northern slope ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-030

    authors: Osono T,Hirose D

    更新日期:2009-07-01 00:00:00

  • Internalization of Mycobacterium shottsii and Mycobacterium pseudoshottsii by Acanthamoeba polyphaga.

    abstract::Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0079

    authors: Gupta T,Fine-Coulson K,Karls R,Gauthier D,Quinn F

    更新日期:2013-08-01 00:00:00

  • Immunovirological studies on human respiratory syncytial virus structural proteins.

    abstract::Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-004

    authors: Trudel M,Nadon F,Séguin C,Ghoubril S,Payment P,Trépanier P

    更新日期:1986-01-01 00:00:00

  • Peptidoglycan turnover during growth recovery after chloramphenicol treatment in a Dap-Lys-mutant of Bacillus megaterium.

    abstract::The study of diaminopimelic acid (DAP) incorporation and turnover during growth recovery in chloramphenicol-treated (CMP-treated) Bacillus megaterium cells showed that two kinds of turnover occurred. A low acid-soluble turnover appeared as soon as growth resumed in bacteria labeled before the CMP treatment and at the ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-051

    authors: Fréhel C,de Chastellier C,Ryter A

    更新日期:1980-03-01 00:00:00

  • The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuary.

    abstract::The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cyto...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2012-0606

    authors: Pereira Md,Byrne BA,Nguyen TB,Lewis DJ,Atwill ER

    更新日期:2013-06-01 00:00:00

  • Transforming the sapstaining fungus Ophiostoma piceae with Agrobacterium tumefaciens.

    abstract::A transformation protocol mediated by Agrobacterium tumefaciens is described for the sapstaining fungus Ophiostoma piceae. We compared transformants obtained from Agrobacterium with those obtained from yeast-like cells made into spheroplasts and treated with CaCl2. For all putative transformants analyzed, Southern hyb...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-036

    authors: Tanguay P,Breuil C

    更新日期:2003-04-01 00:00:00

  • Nitrogen fixation in the muskeg ecosystem of the James Bay Lowlands, Northern Ontario.

    abstract::The acetylene-reduction assay was used for in situ and laboratory assessment of biological nitrogen fixation in the acidic, waterlogged, muskeg ecosystem of the southern James Bay area, in the region of Moosonee, Ontario. In situ assays and subsequent laboratory experimentation revealed that nitrogenase activity was p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-130

    authors: Blasco JA,Jordan DC

    更新日期:1976-07-01 00:00:00

  • Performance of ectomycorrhizal alders exposed to specific Canadian oil sands tailing stressors under in vivo bipartite symbiotic conditions.

    abstract::Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0703

    authors: Beaudoin-Nadeau M,Gagné A,Bissonnette C,Bélanger PA,Fortin JA,Roy S,Greer CW,Khasa DP

    更新日期:2016-07-01 00:00:00

  • Bioflotation of sulfide minerals with Acidithiobacillus ferrooxidans in relation to copper activation and surface oxidation.

    abstract::Surface oxidation of sulfides and copper (Cu) activation are 2 of the main processes that determine the efficiency of flotation. The present study was developed with the intention to ascertain the role of the phenomena in the biomodification of sulfides by Acidithiobacillus ferrooxidans culture (cells and growth me...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-072

    authors: Pecina-Treviño ET,Ramos-Escobedo GT,Gallegos-Acevedo PM,López-Saucedo FJ,Orrantia-Borunda E

    更新日期:2012-09-01 00:00:00

  • Changes in glutathione metabolic enzymes during yeast-to-mycelium conversion of Candida albicans.

    abstract::Candida albicans is a dimorphic yeast capable of producing alternate morphological forms (yeast or mycelium) in response to environmental changes. The intracellular level of glutathione, which helps to maintain the redox potential of the cell, is decreased significantly during the thermal induction of yeast-to-myceliu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-011

    authors: Manavathu M,Gunasekaran S,Porte Q,Manavathu E,Gunasekaran M

    更新日期:1996-01-01 00:00:00

  • Reduction of linoleic acid inhibition in production of conjugated linoleic acid by Propionibacterium freudenreichii ssp. shermanii.

    abstract::A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate deter...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Rainio A,Vahvaselkä M,Suomalainen T,Laakso S

    更新日期:2001-08-01 00:00:00