The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuary.

Abstract:

:The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cytotoxin-encoding genes. Using primers targeting subA only, we generated PCR amplicons from 2 strains; while using primers targeting the 3' end of SubA downstream to the 5' end of SubB, amplicons of 232 bp were generated from 5 additional strains. The 556 bp subA sequences were almost identical to that in the subtilase-cytotoxin-positive strain ED 591 (98%), while subAB sequences of 2 non-Shiga-toxigenic strains revealed 100% similarity with the Shiga-toxigenic E. coli O113:H21 strain 98NK2 that was isolated from an outbreak of hemolytic uremic syndrome. Additionally, the serogroup O113:H21 was present in this collection of environmental E. coli, and it was found to harbor stx2d, hra1 that encodes the heat resistant agglutinin 1, and a subAB sequence similar to that in the non-Shiga-toxigenic E. coli subtilase cytotoxin strain ED 591. To further understand potential health risks posed by strains encoding SubAB, future epidemiological studies should consider screening isolates for subAB regardless of the presence of Shiga-toxin-encoding genes.

journal_name

Can J Microbiol

authors

Pereira Md,Byrne BA,Nguyen TB,Lewis DJ,Atwill ER

doi

10.1139/cjm-2012-0606

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

437-41

issue

6

eissn

0008-4166

issn

1480-3275

journal_volume

59

pub_type

杂志文章
  • Transcriptional regulation of histidine biosynthesis genes in Corynebacterium glutamicum.

    abstract::Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. The latter his operon starts the transcription at the C residue localized...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-115

    authors: Jung S,Chun JY,Yim SH,Lee SS,Cheon CI,Song E,Lee MS

    更新日期:2010-02-01 00:00:00

  • Survival of lactococci during passage through mouse digestive tract.

    abstract::One of the important properties of probiotics is the ability to survive in the intestine. There have been few studies on the probiotic property of lactococci, since they are formally not considered to be natural inhabitants of the intestine. To evaluate lactococci as probiotic bacteria, we investigated their ability t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-092

    authors: Kimoto H,Nomura M,Kobayashi M,Mizumachi K,Okamoto T

    更新日期:2003-11-01 00:00:00

  • Stress-survival responses of a carbon-starved p-nitrophenol-mineralizing Moraxella strain in river water.

    abstract::The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-131

    authors: Moore M,Trevors J,Lee H,Leung KT

    更新日期:2005-03-01 00:00:00

  • Activation of proinflammatory response in human intestinal epithelial cells following Vibrio cholerae infection through PI3K/Akt pathway.

    abstract::Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-093

    authors: Bandyopadhaya A,Bhowmick S,Chaudhuri K

    更新日期:2009-11-01 00:00:00

  • Nutrient uptake by Candida albicans: the influence of cell surface mannoproteins.

    abstract::Numerous ultrastructural and biochemical analyses have been performed to characterize the cell wall composition and structure of Candida albicans. However, little investigation has focused on how subtle differences in cell wall structure influence the intracellular transport of amino acids and monosaccharides. In this...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Braun PC

    更新日期:1999-05-01 00:00:00

  • Efficient production of Aschersonia placenta protoplasts for transformation using optimization algorithms.

    abstract::The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0770

    authors: Wei X,Song X,Dong D,Keyhani NO,Yao L,Zang X,Dong L,Gu Z,Fu D,Liu X,Qiu J,Guan X

    更新日期:2016-07-01 00:00:00

  • A diaminopimelic acid auxotrophic Escherichia coli donor provides improved counterselection following intergeneric conjugation with actinomycetes.

    abstract::Considering the medical, biotechnological, and economical importance of actinobacteria, there is a continuous need to improve the tools for genetic engineering of a broad range of these microorganisms. Intergeneric conjugation has proven to be a valuable yet imperfect tool for this purpose. The natural resistance of m...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0041

    authors: Allard N,Garneau D,Poulin-Laprade D,Burrus V,Brzezinski R,Roy S

    更新日期:2015-08-01 00:00:00

  • Bacteriocin production by Streptococcus salivarius strain P.

    abstract::A bacteriocin, streptococcin sal-P, was isolated by freeze-thaw elution from cultures of Streptococcus salivarius strain P grown on tryptic soy agar plus 1% neopeptone. The inhibitor could also be extracted with either 7 M urea of 1 M NaCl from cells grown on this medium, but little activity was recovered from cells g...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-144

    authors: Tagg JR,Russell C

    更新日期:1981-09-01 00:00:00

  • Thiobacillus acidophilus: a study of its presence in Thiobacillus ferrooxidans cultures.

    abstract::A study has been undertaken to account for the presence of Thiobacillus acidophilus in iron-grown cultures of Thiobacillus ferrooxidans. Attempts to adapt T. acidophilus to ferrous iron were not successful but the facultative autotroph grew to a limited extent in the spent medium of T. ferrooxidans and was able to gro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-178

    authors: Arkesteyn GJ,de Bont JA

    更新日期:1980-09-01 00:00:00

  • Antibiotic efficacy in intraabdominal sepsis: a clinically relevant model.

    abstract::We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-059

    authors: Bohnen JM,Matlow AG,Mustard RA,Christie NA,Kavouris B

    更新日期:1988-03-01 00:00:00

  • Exopolysaccharide depolymerases induced by Rhizobium bacteriophages.

    abstract::Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-239

    authors: Barnet YM,Humphrey B

    更新日期:1975-10-01 00:00:00

  • Deoxyribonucleic acid relationships among members of the genus Aeromonas.

    abstract::Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-083

    authors: MacInnes JI,Trust TJ,Crosa JH

    更新日期:1979-05-01 00:00:00

  • Rapid comparison of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris using polymerase chain reaction amplification.

    abstract::Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequenc...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m93-059

    authors: Kwoh DY,Vedvick TS,McCue AF,Gevertz D

    更新日期:1993-04-01 00:00:00

  • Change in antimicrobial susceptibility of Escherichia coli urinary tract isolates at a single institution over a period of 10 years.

    abstract::Urinary tract infections are common. Few published studies have demonstrated the change in Escherichia coli urinary isolate antimicrobial susceptibility over time within a given area and (or) population. The purpose of this study was to evaluate the change in susceptibility of E. coli clinical isolates obtained from u...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-001

    authors: Walkty A,Lagacé-Wiens PR,Karlowsky JA,Hoban DJ,Manickam K,Adam H,Pieroni P,Alfa M

    更新日期:2012-03-01 00:00:00

  • Cell surface properties of two differently virulent strains of Acinetobacter baumannii isolated from a patient.

    abstract::The aim of this study was to unravel, by focusing on cell surface properties, the underlying virulence factors contributing to the difference in the pathogenicity observed in two Acinetobacter baumannii strains isolated from the same patient. The two strains were phenotypically different: (i) a mucoid strain (AB-M),...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-131

    authors: Kempf M,Eveillard M,Deshayes C,Ghamrawi S,Lefrançois C,Georgeault S,Bastiat G,Seifert H,Joly-Guillou ML

    更新日期:2012-03-01 00:00:00

  • Isolation of enterotoxigenic Aeromonas from fish.

    abstract::This paper reports two different enterotoxigenic classes of the genus Aeromonas isolated from fishes. Strains of A. sobria were isolated from healthy fishes only wheras strains of A. hydrophila were isolated from both healthy and moribund fishes. Aeromonas sobria and hydrophilia strains produced a cytotoxic factor and...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m77-174

    authors: Boulanger Y,Lallier R,Cousineau G

    更新日期:1977-09-01 00:00:00

  • [Metabolic activity of Candida lipolytica adsorbed to bentonite with hydrophobic chains].

    abstract::The respiration of Candida lipolytica on n-tetradecane is decreased in the presence of bentonite. This inhibition is less pronounced by the introduction of hydrophobic chains on the bentonite. Oxygen demand of resting cells varies with the length of hydrophobic chains. In fermentor, addition of adsorbant does not stim...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Pareilleux A,Maignan C

    更新日期:1976-08-01 00:00:00

  • A N2-fixing endophytic Burkholderia sp. associated with maize plants cultivated in Mexico.

    abstract::In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied. Under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-023

    authors: Estrada P,Mavingui P,Cournoyer B,Fontaine F,Balandreau J,Caballero-Mellado J

    更新日期:2002-04-01 00:00:00

  • Colony counts and characterization of bacteria adherent to the rumen wall and desquamated epithelial cells in conventional young lambs.

    abstract::Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial ce...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-114

    authors: Rieu F,Fonty G,Gouet P

    更新日期:1989-07-01 00:00:00

  • Antibacterial activity of BCG-induced, interferon-containing sera.

    abstract::Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-084

    authors: Moulton RG,Gerner GD

    更新日期:1986-05-01 00:00:00

  • Bacteriophage lambda repressor allelic modulation of the Rex exclusion phenotype.

    abstract::The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-re...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-021

    authors: Slavcev RA,Bull HJ,Hayes S

    更新日期:2003-03-01 00:00:00

  • Alkaline phosphatase and a cellulase reporter protein are not exported from the cytoplasm when fused to large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein.

    abstract::Using a gene fusion approach, hybrid proteins were created by linking alkaline phosphatase (PhoA) or a cellulase reporter (delta CenA) to four large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein (RsaA; 1026 amino acids). Three of the sites (amino acids 189, 220, 315) were selected on the ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m94-122

    authors: Bingle WH,Smit J

    更新日期:1994-09-01 00:00:00

  • Polycyclic aromatic hydrocarbon degrading gene islands in five pyrene-degrading Mycobacterium isolates from different geographic locations.

    abstract::Mycobacterium sp. strain KMS utilizes pyrene, a high-molecular weight polycyclic aromatic hydrocarbon (PAH), as a sole carbon source. Bioinformatic analysis of the genome of isolate KMS predicted 25 genes with the potential to encode 17 pyrene-induced proteins identified by proteomics; these genes were clustered on b...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-093

    authors: Zhang C,Anderson AJ

    更新日期:2012-01-01 00:00:00

  • Protein analysis in a pleomorphically deteriorated strain of the insect-pathogenic fungus Metarhizium anisopliae.

    abstract::Pleomorphic deterioration is a process where a fungal isolate loses the ability to produce conidia during repeated subculturing. We have previously isolated strains of the entomopathogenic fungus Metarhizium anisopliae that have irreversibly lost the ability to produce conidia and only produce mycelia when grown on ag...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-074

    authors: Kamp AM,Bidochka MJ

    更新日期:2002-09-01 00:00:00

  • Control of pyrimidine formation in Pseudomonas putida ATCC 17536.

    abstract::The regulation of de novo pyrimidine biosynthesis in Pseudomonas putida ATCC 17536 by pyrimidines was explored. The pathway enzyme activities were higher in glucose-grown cells than in succinate-grown cells, indicating catabolite repression by succinate. In P. putida cells grown on succinate as a carbon source, only a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-110

    authors: Santiago MF,West TP

    更新日期:2002-12-01 00:00:00

  • Genomic comparison of facultatively anaerobic and obligatory aerobic Caldibacillus debilis strains GB1 and Tf helps explain physiological differences.

    abstract::Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0464

    authors: Wushke S,Froese A,Fristensky B,Zhang XL,Spicer V,Krokhin OV,Levin DB,Sparling R

    更新日期:2019-06-01 00:00:00

  • Importance of the icmN gene in the growth of Legionella pneumophila in amoebic cells at low temperature.

    abstract::Legionella pneumophila grows in amoebae and has achieved the ability to grow at various temperatures, although the mechanisms controlling this ability remain poorly understood. The Icm/Dot type IVB secretion system is composed of more than 25 proteins and is known to be essential for intracellular growth. The role of...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-011

    authors: Qin T,Iida K,Piao Z,Shiota S,Ren H,Shao Z,Yoshida S

    更新日期:2012-04-01 00:00:00

  • Characterization of the extracellular poly(3-hydroxybutyrate) depolymerase of Comamonas sp. and of its structural gene.

    abstract::The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m95-183

    authors: Jendrossek D,Backhaus M,Andermann M

    更新日期:1995-01-01 00:00:00

  • A novel approach for the improvement of ethanol fermentation by Saccharomyces cerevisiae.

    abstract::Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involv...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-032

    authors: Hou L,Cao X,Wang C

    更新日期:2010-06-01 00:00:00

  • [Presence of Pseudomonas maltophilia in the rhizosphere of several cultivated plants].

    abstract::Pseudomonas maltophilia has been identified in the rhizosphere of several cultured plants: cabbage, rape, mustard, corn, beet. In comparison with nonrhizospheric soil, all the rhizospheric samples analysed contain more of this Pseudomonas and the most important stimulation is supplied by cruciferous plants. The possib...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Debette J,Blondeau R

    更新日期:1980-04-01 00:00:00