Abstract：
:We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excision and peritoneal lavage, 18 h after the onset of infection. This is different from previous models of abdominal sepsis (in which antibiotics are given within 4 h of peritoneal contamination) and mimics the clinical setting in which antibiotics are initiated much later, at the time of operation. Antibiotic-treated rats received 20 mg cefoxitin i.m. every 8 h for 7 days; controls received saline at similar times. Thirty-nine of 67 control rats died (58%) versus 20 of 64 (31%) that received cefoxitin (p less than 0.005). We conclude that even with delayed administration, antibiotics appear to improve the outcome of intraabdominal sepsis. With further characterization of this model we plan to use it as an in vivo assay to compare the efficacy of different antimicrobial agents in intraabdominal sepsis.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Bohnen JM,Matlow AG,Mustard RA,Christie NA,Kavouris Bdoi
10.1139/m88-059subject
Has Abstractpub_date
1988-03-01 00:00:00pages
323-6issue
3eissn
0008-4166issn
1480-3275journal_volume
34pub_type
杂志文章abstract：:The effect of fructans on the cultivable faecal community of Bio Breeding rats fed diets containing 5% (m/v) food-grade fructooligosaccharide (FOS) was investigated. Culturing of faecal material using chicory inulin as the sole carbohydrate source revealed the presence of a greater diversity of inulin-utilizing bacter...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-053
更新日期：2006-10-01 00:00:00
abstract：:Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-016
更新日期：1993-01-01 00:00:00
abstract：:The prevalence of Coxiella burnetti infection (Q fever) was determined among Nova Scotia (N.S.) and Prince Edward Island (P.E.I.) blood donors by using the complement fixation and microimmunofluorescence (IF) test. The complement fixation and IF antibody tests measured antibody prevalence for the phase II or phase I a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-021
更新日期：1984-01-01 00:00:00
abstract：:Fourteen Lactococcus lactis strains showing inhibitory activity against Listeria innocua SICC 4202 were isolated from different French raw milks and raw milk cheeses and screened for bacteriocin production by the triple layer method under conditions that eliminate the effects of lactic acid and hydrogen peroxide. Thre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-114
更新日期：1995-09-01 00:00:00
abstract：:Environmental scanning electron microscopic observations of Postia placenta grown on a defined medium and on red spruce wood allowed for the examination of the hydrated sheath of P. placenta. In the wood environment, mature hyphae that were not adhering to the substrate were observed to have a mycofibrillar morphology...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-058
更新日期：1995-04-01 00:00:00
abstract：:Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-051
更新日期：1982-03-01 00:00:00
abstract：:Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-093
更新日期：2009-11-01 00:00:00
abstract：:Symbiotic nitrogen fixation may be limited by the transport of C4 dicarboxylates into bacteroids in the nodule for use as a carbon and energy source. In an attempt to increase dicarboxylate transport, a plasmid was constructed in which the Rhizobium meliloti structural transport gene dctA was fused to a tryptophan ope...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-091
更新日期：1992-06-01 00:00:00
abstract：:Attempts at improving chemical fixation for electron-microscopic observation of the capsule of Klebsiella pneumoniae were made. The capsule was preserved by using alcian blue - lanthanum and tris-(1-aziridinyl) phosphine oxide (TAPO) - aldehyde - osmium procedures. Despite the different retention of the overall capsul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-102
更新日期：1977-06-01 00:00:00
abstract：:The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigen...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-043
更新日期：1989-02-01 00:00:00
abstract：:The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-100
更新日期：1989-06-01 00:00:00
abstract：:Sixty-four species of filamentous fungi from five flare pits in northern and western Canada were tested for their ability to degrade crude oil using gas chromatographic analysis of residual hydrocarbons following incubation. Nine isolates were tested further using radiorespirometry to determine the extent of mineraliz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Five media and procedures were compared for their recovery of fecal streptococci on membrane filters. Growth and numbers of a variety of pure cultures were compared, and recoveries from raw sewage and sewage effluent were determined on the five media. A total of 512 isolates were identified and the specificity of each...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-219
更新日期：1980-11-01 00:00:00
abstract：:Several strains of Rhizobium japonicum have been reported to consist of mixtures of stable derivativess having distinct colony morphologies and physiological characteristics. We isolated derivatives from strains of R. japonicum and systematically compared them with previously isolated derivatives with respect to the u...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-106
更新日期：1980-05-01 00:00:00
abstract：:Acid (AcP) and alkaline phosphatase (AlP) were localized by physicobiochemical techniques. Greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted Capnocytophaga species released only 9-28% and 11-43% of the cellular pho...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-212
更新日期：1983-10-01 00:00:00
abstract：:The range of polysaccharide-degrading enzymes formed by three anaerobic rumen fungi (Neocallimastix patriciarum, Piromonas communis, and an unidentified isolate (F] was monitored following growth on seven mono-, di-, and poly-saccharide carbohydrate substrates. Enzymes capable of degrading a variety of alpha- and beta...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-071
更新日期：1987-05-01 00:00:00
abstract：:The influence of plant growth promoting (PGP) activity of bacterial communities recovered from each of six weed species (barnyard grass (Echinochloa crusfalli (L.) Beauv.), corn spurrey (Spergula arvensis L.), goldenrod (Sonchus sp.), Italian ryegrass (Lolium multiflorum L.), lamb's-quarters (Chenopodium album L.), an...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-110
更新日期：2001-11-01 00:00:00
abstract：:Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0763
更新日期：2015-03-01 00:00:00
abstract：:Administered orally, Quillaja saponin markedly potentiated the humoral immune responses of mice fed inactivated rabies vaccine, and significantly increased their resistance to subsequent intracerebral challenge with live rabies virus. Although mean serum neutralizing antibody titres were generally 8- to 16-fold higher...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-078
更新日期：1986-05-01 00:00:00
abstract：:The nucleotide sequence of the murE gene encoding the diaminopimelic acid adding enzyme of Escherichia coli is reported. The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs. The deduced primary structure of MurE comprised 471 amino acid re...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-175
更新日期：1989-11-01 00:00:00
abstract：:A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-162
更新日期：1997-12-01 00:00:00
abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-129
更新日期：2002-01-01 00:00:00
abstract：:The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-012
更新日期：1975-01-01 00:00:00
abstract：:The gene for a type IIo IgG-binding protein has previously been cloned and sequenced. The approximately 60,000 M(r) recombinant gene product binds all four human IgG subclasses and fibrinogen. Treatment of this recombinant protein with CNBr results in generation of a series of fragments. One fragment, an approximately...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-149
更新日期：1996-11-01 00:00:00
abstract：:In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-157
更新日期：2009-05-01 00:00:00
abstract：:This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-059
更新日期：2004-09-01 00:00:00
abstract：:The multi-drug resistant pathogen Acinetobacter baumannii displays unusual control of its SOS mutagenesis genes, as it does not encode a LexA repressor, but instead employs the UmuDAb repressor and a small DdrR protein that is uniquely found in Acinetobacter species. We used bacterial adenylate cyclase t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0219
更新日期：2020-11-12 00:00:00
abstract：:Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-094
更新日期：2000-11-01 00:00:00
abstract：:Fine hair-like appendages on the cell walls of the another smut Ustilago violacea are described. These hairs are termed fimbriae because of their close similarity to the fimbriae (pili) found on certain Gram-negative bacteria. Cells of U. violacea may carry more than 200 fimbriae varying in length from about 0.5 mum t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-076
更新日期：1975-04-01 00:00:00
abstract：:Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-201
更新日期：1980-10-01 00:00:00
