Effects of culture conditions on yield of Shiga-like toxin-IIv from Escherichia coli.

abstract：:An anaerobic consortium that carboxylated and dehydroxylated phenol to benzoate, and 2-cresol to 3-methylbenzoic acid, under methanogenic conditions was studied. Phenol induced this transformation activity. Addition of 4-hydroxypyridine or an increase in the concentration of proteose peptone to 0.5% (w/v) delayed the ...

journal_title：Canadian journal of microbiology

authors： Bisaillon JG,Lépine F,Beaudet R,Sylvestre M

更新日期：1993-07-01 00:00:00

abstract：:An obligate methyltroph Methylomonas methylovora oxidized methylamine, formaldehyde, and formate. Enzymes oxidizing these substrates were detected in a cell-free system. Phenazine methosulfate-linked methylamine dehydrogenase was purified 21-fold. The enzyme had optimum activity at pH 7.5 and was stable at 60 degrees ...

journal_title：Canadian journal of microbiology

authors： Mehta RJ

更新日期：1977-04-01 00:00:00

abstract：:A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...

journal_title：Canadian journal of microbiology

authors： Lawler GC,Weber DJ

更新日期：1977-08-01 00:00:00

abstract：:We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...

journal_title：Canadian journal of microbiology

authors： Xiang H,Wei GF,Jia S,Huang J,Miao XX,Zhou Z,Zhao LP,Huang YP

更新日期：2006-11-01 00:00:00

abstract：:Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...

journal_title：Canadian journal of microbiology

authors： Lapierre J,Marsolais G,Pilon P,Descôteaux JP

更新日期：1980-10-01 00:00:00

abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...

journal_title：Canadian journal of microbiology

authors： White AP,Allen-Vercoe E,Jones BW,DeVinney R,Kay WW,Surette MG

更新日期：2007-01-01 00:00:00

abstract：:Aluminum (Al) toxicity is a major problem affecting soil fertility, microbial diversity, and nutrient uptake of plants. Rhizobia response and legume interaction under Al conditions are still unknown; it is important to understand how to develop and improve legume cultivation under Al stress. In this study, rhizobia re...

journal_title：Canadian journal of microbiology

authors： Artigas Ramírez MD,Silva JD,Ohkama-Ohtsu N,Yokoyama T

更新日期：2018-08-01 00:00:00

abstract：:The effect of fructans on the cultivable faecal community of Bio Breeding rats fed diets containing 5% (m/v) food-grade fructooligosaccharide (FOS) was investigated. Culturing of faecal material using chicory inulin as the sole carbohydrate source revealed the presence of a greater diversity of inulin-utilizing bacter...

journal_title：Canadian journal of microbiology

authors： Gourgue-Jeannot C,Kalmokoff ML,Kheradpir E,Kwan J,Lampi BJ,McAllister M,Brooks SP

更新日期：2006-10-01 00:00:00

abstract：:The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...

journal_title：Canadian journal of microbiology

authors： Bannatyne RM,Cheung R

更新日期：1980-02-01 00:00:00

abstract：:Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...

journal_title：Canadian journal of microbiology

authors： Schippa S,Iebba V,Totino V,Santangelo F,Lepanto M,Alessandri C,Nuti F,Viola F,Di Nardo G,Cucchiara S,Longhi C,Conte MP

更新日期：2012-04-01 00:00:00

abstract：:The regulation of de novo pyrimidine biosynthesis in Pseudomonas putida ATCC 17536 by pyrimidines was explored. The pathway enzyme activities were higher in glucose-grown cells than in succinate-grown cells, indicating catabolite repression by succinate. In P. putida cells grown on succinate as a carbon source, only a...

journal_title：Canadian journal of microbiology

authors： Santiago MF,West TP

更新日期：2002-12-01 00:00:00

abstract：:Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...

journal_title：Canadian journal of microbiology

authors： Castric PA

更新日期：1983-10-01 00:00:00

abstract：:The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...

journal_title：Canadian journal of microbiology

authors： Kudo H,Jakober KD,Phillippe RC,Cheng KJ,Barr DJ,Costerton JW

更新日期：1990-07-01 00:00:00

abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...

journal_title：Canadian journal of microbiology

authors： MacNeil L,Kauri T,Robertson W

更新日期：1995-08-01 00:00:00

abstract：:DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschniko...

journal_title：Canadian journal of microbiology

authors： Mendonça-Hagler LC,Hagler AN,Phaff HJ,Tredick J

更新日期：1985-10-01 00:00:00

abstract：:Vero cells were more suitable then BHK-21 cells for the detection of rubella-specific IgG antibodies because the nonspecific fluorescence was minimal. However, BHK-21 cells were found more sensitive than Vero cells for the detection of rubella-specific IgM antibodies. ...

journal_title：Canadian journal of microbiology

authors： Payment P,Roy L,Gilker JC,Chagnon A

更新日期：1975-03-01 00:00:00

abstract：:One of the important properties of probiotics is the ability to survive in the intestine. There have been few studies on the probiotic property of lactococci, since they are formally not considered to be natural inhabitants of the intestine. To evaluate lactococci as probiotic bacteria, we investigated their ability t...

journal_title：Canadian journal of microbiology

authors： Kimoto H,Nomura M,Kobayashi M,Mizumachi K,Okamoto T

更新日期：2003-11-01 00:00:00

abstract：:For the recently described serotype 15 of biotype I and serotypes 13 and 14 of biotype II of Actinobacillus pleuropneumoniae, fhuA and hgbA were detected by polymerase chain reaction and DNA sequencing. To determine the substrate specificity of the iron receptors FhuA and HgbA and to study their role in the virulence ...

journal_title：Canadian journal of microbiology

authors： Shakarji L,Mikael LG,Srikumar R,Kobisch M,Coulton JW,Jacques M

更新日期：2006-04-01 00:00:00

abstract：:The phototrophic purple sulfur bacterium Thiopedia rosea forms multicellular, gas-vacuolate, regular, flat aggregates (platelets, sheets) held together by slime. Platelets found in eutrophic water consisted of slime (85% of the total wet volume) and 16 cells, while the gas-filled vacuole occupied 44% of the volume of ...

journal_title：Canadian journal of microbiology

authors： Scherrer R,Shull VE

更新日期：1986-07-01 00:00:00

abstract：:Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...

journal_title：Canadian journal of microbiology

authors： Davidson EW,Shellabarger C,Meyer M,Bieber AL

更新日期：1987-11-01 00:00:00

abstract：:Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...

journal_title：Canadian journal of microbiology

authors： McLoughlin TJ,Alt SG,Merlo PA

更新日期：1990-11-01 00:00:00

abstract：:Nonmotile and motility-altered mutants of Myxococcus xanthus have been obtained by the use of chemical mutagens, ultraviolet irradiation, and a procedure for selective spontaneous mutants. As judged by their behaviour on a variety of growth media, in both plate and slide culture, the mutants were divided into four gro...

journal_title：Canadian journal of microbiology

authors： MacRae TH,McCurdy HD

更新日期：1976-09-01 00:00:00

abstract：:The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...

journal_title：Canadian journal of microbiology

authors： Vachon V,Kristjanson DN,Coulton JW

更新日期：1988-02-01 00:00:00

abstract：:A defined medium (CDM) is described which supported growth and sporulation of type E strains of Clostridium botulinum, but not sporulation of other serotypes of C. botulinum or C. sporogenes. As compared to growth in complex medium, spore outgrowth was delayed and both the growth rate and the cell yield was reduced. H...

journal_title：Canadian journal of microbiology

authors： Hawirko RZ,Naccarato CA,Lee RP,Maeba PY

更新日期：1979-04-01 00:00:00

abstract：:The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...

journal_title：Canadian journal of microbiology

authors： Gilchrist A,Fisher JA,Smit J

更新日期：1992-03-01 00:00:00

abstract：:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...

journal_title：Canadian journal of microbiology

authors： Regenstreif CA,Kelland JG,Pansare SV,Vederas JC,Pickard MA

更新日期：1986-06-01 00:00:00

abstract：:The cultural, morphological, and physiological properties of Thermomonospora fusca (strain 190Th) are described. Its physiological properties show that this species is primarily a carbohydrate-degrading actinomycete which can use a wide range of plant sugars and polymeric carbohydrates as sources of carbon and energy....

journal_title：Canadian journal of microbiology

authors： Crawford DL

更新日期：1975-11-01 00:00:00

abstract：:To develop a library-dependent method of tracking fecal sources of contamination of beaches on the Atlantic coast of southwestern France, a library of 6368 Escherichia coli isolates was constructed from samples of feces, from 40 known human or animal sources collected in the vicinity of Arcachon Bay in 2010, and in Fr...

journal_title：Canadian journal of microbiology

authors： Garabetian F,Vitte I,Sabourin A,Moussard H,Jouanillou A,Mornet L,Lesne M,Lyautey E

更新日期：2020-12-01 00:00:00

abstract：:Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 microgram of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as ca...

journal_title：Canadian journal of microbiology

authors： Liao CH,Heberlein GT

更新日期：1979-02-01 00:00:00

abstract：:We report some characteristics of a ruminal bacterium (strain NPOH1) that metabolizes 3-nitropropanol, the toxic principle of various milk vetchs that are distributed worldwide. The gram-positive bacterium was nonmotile and did not produce spores. Growth of strain NPOH1 occurred under anaerobic conditions and was supp...

journal_title：Canadian journal of microbiology

authors： Anderson RC,Rasmussen MA,DiSpirito AA,Allison MJ

更新日期：1997-07-01 00:00:00