Abstract：
:Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhanced adhesive and invasive properties, mainly due to the mannose-bonding FimH protein. We aimed to characterize 52 mucosa-associated E. coli strains isolated from pediatric IBD and non-IBD patients. Eleven E. coli strains, showing a strong similarity in fimH gene sequence to that of E. coli AIEC LF82, were characterized for fimH gene sequence, genomic profiling, adhesive and invasive ability, and phylogrouping. The results were compared with E. coli strains AIEC LF82 and MG1655. The 11 E. coli isolates showed 82.4% ± 1.4% fimH sequence similarity and 80.6% ± 1.3% genomic similarity to strain AIEC LF82. All these strains harbored V27A and S78N FimH mutations, as found in LF82. Nine of them belonged to the more virulent B2 and D phylogroups. Neuraminidase treatment, mimicking inflamed mucosa, enhanced adhesion of all 11 strains by 3.5-fold, but none showed invasion ability. It could be argued that the 11 selected strains could be a branch of an E. coli subpopulation (pathobionts), that could take advantage in an inflamed context because of a suitable genomic and (or) genetic backdrop.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Schippa S,Iebba V,Totino V,Santangelo F,Lepanto M,Alessandri C,Nuti F,Viola F,Di Nardo G,Cucchiara S,Longhi C,Conte MPdoi
10.1139/w2012-007subject
Has Abstractpub_date
2012-04-01 00:00:00pages
426-32issue
4eissn
0008-4166issn
1480-3275journal_volume
58pub_type
杂志文章abstract：:This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-001
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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pub_type： 杂志文章
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-135
更新日期：2006-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-027
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0612
更新日期：2013-06-01 00:00:00
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更新日期：2006-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-043
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journal_title：Canadian journal of microbiology
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doi：10.1139/cjm-2015-0710
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doi：10.1139/cjm-2019-0343
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-122
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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journal_title：Canadian journal of microbiology
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