Abstract：
:Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 microgram of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as carrot, potato, and pinto bean were 45--75% less than those induced by PBLs wild types. The crude cell envelopes (CCE) prepared from both PBLs and PBLr bacteria were inhibitory for tumor initiation when they were applied before or during the inoculation of viable tumorigenic bacteria, but not when they were applied 30 min after the inoculation of infectious bacteria. The potency to inhibit the tumor initiation by the CCE prepared from PBLs cells was approximately 50% higher than that by the equal amount of the CCE prepared from PBLr cells. The concentration of CCE preparations required to reduce tumor induction 50% in carrot and pinto bean was determined to be 2.6 mg/mL and 4.0--6.2 mg/mL for the CCE derived from PBLs and PBLr cells, respectively. These data suggest that the envelope structure or composition of PBLs and PBLr cells is distinct, and that the acquisition of resistance to polymyxin by agrobacteria modifies envelope structure or components which are essential for tumor initiation.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Liao CH,Heberlein GTdoi
10.1139/m79-029subject
Has Abstractpub_date
1979-02-01 00:00:00pages
185-91issue
2eissn
0008-4166issn
1480-3275journal_volume
25pub_type
杂志文章abstract：:Bacterial biofilms are commonly formed on medical devices and food processing surfaces. The antimicrobials used have limited efficacy against the biofilms; therefore, new strategies to prevent and remove these structures are needed. Here, the effectiveness of brief oxidative treatments, based on the combination of sod...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0747
更新日期：2015-05-01 00:00:00
abstract：:This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-059
更新日期：2004-09-01 00:00:00
abstract：:Vero cells were more suitable then BHK-21 cells for the detection of rubella-specific IgG antibodies because the nonspecific fluorescence was minimal. However, BHK-21 cells were found more sensitive than Vero cells for the detection of rubella-specific IgM antibodies. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-041
更新日期：1975-03-01 00:00:00
abstract：:Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-062
更新日期：2006-10-01 00:00:00
abstract：:Klebsiella pneumoniae was found to effect the reductive dechlorination of methoxychlor (2,2-bis(p-methoxyphenyl)-1, 1, 1-trichloroethane) and its dihydroxy derivative (2, 2-bis (p-hydroxyphenyl)-1, 1, 1-trichloroethane) to dichloro derivatives 2, 2-bis (p-methoxyphenyl)-1, 1-dichloroethane and 2, 2-bis (p-hydroxypheny...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-023
更新日期：1982-02-01 00:00:00
abstract：:In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-079
更新日期：1978-04-01 00:00:00
abstract：:Human astrovirus (HAstV) was an important cause of viral gastroenteritis in infants in Wuhan city based on our previous study. The aim of the study was to investigate the nature of HAstV infection in Wuhan, People's Republic of China, especially in adults. Stool specimens were collected from 361 children and 301 adult...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-085
更新日期：2011-11-01 00:00:00
abstract：:Ribosomal preparations from Neisseria gonorrhoeae types 1 and 4 were examined for their in vitro stimulation of mouse splenocytes to determine the ribosomal moiety or contaminant responsible for the immunoproliferative activity. In immunodiffusion tests with homologous rabbit antiserum, crude 70S ribosomes formed four...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-094
更新日期：1978-05-01 00:00:00
abstract：:Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transpo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-021
更新日期：1991-02-01 00:00:00
abstract：:The oligosaccharides of human lactoferrin were enzymatically removed with glycopeptidase F, resulting in a preparation containing partial and fully deglycosylated human lactoferrin. The derivatives were separated by Concanavalin A affinity chromatography and compared with native human lactoferrin with respect to their...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-198
更新日期：1992-11-01 00:00:00
abstract：:Administered orally, Quillaja saponin markedly potentiated the humoral immune responses of mice fed inactivated rabies vaccine, and significantly increased their resistance to subsequent intracerebral challenge with live rabies virus. Although mean serum neutralizing antibody titres were generally 8- to 16-fold higher...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-078
更新日期：1986-05-01 00:00:00
abstract：:Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-227
更新日期：1976-10-01 00:00:00
abstract：:Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-173
更新日期：1987-11-01 00:00:00
abstract：:Metarhizium anisopliae isolates have a wide insect host range, but an impediment to their commercial use as a biocontrol agent of above-ground insects is the high susceptibility of spores to the near-UV present in solar irradiation. To understand stress responses in M. anisopliae, we initiated studies of enzymes that ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-097
更新日期：2004-01-01 00:00:00
abstract：:Isoquinoline is a nitrogen heterocyclic compound that is associated with coal- and oil-derived wastes. Four strains of bacteria able to degrade isoquinoline in pure culture were isolated from sites known to be contaminated with oil. Isoquinoline was used as the sole source of carbon and nitrogen by these isolates. Iso...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-089
更新日期：1994-07-01 00:00:00
abstract：:Yersinia enterocolitica biotype 1A (B1A) strains are considered mainly nonpathogenic. However, some studies considered strains of this biotype to be the causal agents of infections in humans and animals. In South America, there are no studies that have compared clinical and nonclinical strains of B1A typed by pulsed-f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0211
更新日期：2014-06-01 00:00:00
abstract：:The physiological characteristics and fatty acid composition of 40 strains of Yarrowia lipolytica isolated from irradiated poultry meat, commercial chilled foods, the superficial water of lagoons of an Italian river delta, and commercial light butter were compared. Discriminant analysis of the results permitted the 40...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-008
更新日期：1994-01-01 00:00:00
abstract：:The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W06-124
更新日期：2007-03-01 00:00:00
abstract：:Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed tha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w07-116
更新日期：2008-02-01 00:00:00
abstract：:The techniques in current use for detection of pathogens in environmental samples are restricted to those organisms whose replication in either culture media or cell culture is feasible. These methods lack the selectivity and sensitivity necessary for their unequivocal detection and identification. We have developed a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-062
更新日期：1992-05-01 00:00:00
abstract：:Melanin biosynthesis in Verticillium dahliae Kleb, was studied with mutants deficient for normal black melanin or for production of microsclerotia. Seven genetically different mutants had apparent blocks in melanin biosynthesis. Four mutants (brm-1 to -4) produced brown microsclerotia and extruded pigments into media;...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-115
更新日期：1976-06-01 00:00:00
abstract：:The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-112
更新日期：2004-02-01 00:00:00
abstract：:The aim of the present study was to examine soil samples from various vegetation zones in terms of physicochemical properties, microbial communities, and isolation and identification (by polymerase chain reaction and transmission electron microscopy) of bacteria producing poly-β-hydroxybutyrates (PHBs). Soil samples w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-037
更新日期：2012-05-01 00:00:00
abstract：:For the recently described serotype 15 of biotype I and serotypes 13 and 14 of biotype II of Actinobacillus pleuropneumoniae, fhuA and hgbA were detected by polymerase chain reaction and DNA sequencing. To determine the substrate specificity of the iron receptors FhuA and HgbA and to study their role in the virulence ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-135
更新日期：2006-04-01 00:00:00
abstract：:Various soil fungi were tested for their capacity to degrade the insecticide [14C]photodieldrin. Of nine species investigated, Trichoderma viride was the only one which degraded the insecticide to an appreciable extent into water-soluble, non-insecticidal compounds within 4-5 weeks. These products amounted to 32-41% o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-198
更新日期：1976-09-01 00:00:00
abstract：:Escherichia coli is capable of producing several virulence factors involved in the pathophysiology of bovine mastitis. Cell surface structures such as flagella, pili, and other outer membrane adhesins are considered essential for biofilm formation on abiotic surfaces. The aim of this study was characterize the molecul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0053
更新日期：2013-05-01 00:00:00
abstract：:Aspergillus caespitosus NRRL 5769 growing in broth containing small amounts of sitosterol produced substance(s) with greater inhibitory activity against Candida albicans ATCC 10231 than in broth without sitosterol. Subsequent isolation, purification, and structural elucidation yielded 5,6-dihydro-5(S)-acetoxy-6(S)-1,2...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-259
更新日期：1975-11-01 00:00:00
abstract：:Ten bacterial strains were isolated by enrichment culture, using as carbon sources either aliphatics or an aromatic-polar mixture. Oxygen uptake rate was used as a criterion to determine culture transfer timing at each enrichment stage. Biodegradation of aliphatics (10,000 mg L(-1)) and an aromatic-polar mixture (5000...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-098
更新日期：2003-12-01 00:00:00
abstract：:Mycoplasma pneumoniae is an important causative agent of atypical pneumonia. This study was to determine the ability of a DNA expression vector, which encodes the carboxy terminal region of the M. pneumoniae P1 protein (P1C), to induce humoral and cellular immune responses and to protect against M. pneumoniae infecti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-041
更新日期：2012-05-01 00:00:00
