Inhibition of L-lysine uptake by alpha-methyl lysine in Escherichia coli and Bacillus sphaericus.

Abstract:

:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of lysine uptake in both bacteria. A similar inhibitory effect was observed with epsilon-N-methyl lysine on lysine uptake in B. sphaericus, but not in E. coli. alpha-Methyl lysine had a reduced effect on ornithine uptake and no effect on arginine transport in either bacterium.

journal_name

Can J Microbiol

authors

Regenstreif CA,Kelland JG,Pansare SV,Vederas JC,Pickard MA

doi

10.1139/m86-095

subject

Has Abstract

pub_date

1986-06-01 00:00:00

pages

522-4

issue

6

eissn

0008-4166

issn

1480-3275

journal_volume

32

pub_type

杂志文章
  • Light microscopy observations of tetrazolium-reducing bacteria in the endorhizosphere of maize and other grasses in Brazil.

    abstract::Roots of field-grown tropical maize, Panicum maximum Jacq. and Digitaria decumbens Stent., and of sorghum and wheat grown in monoxenic culture with the diazotroph Spirillum lipoferum (syn. Azospirillum spp.) were examined for tetrazolium-reducing bacteria following incubation of roots in a malate-phosphate buffer-2,3,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-122

    authors: Patriquin DG,Döbereiner J

    更新日期:1978-06-01 00:00:00

  • Cryptococcus festucosus sp. nov. a new hymenomycetous yeast in the Holtermannia clade.

    abstract::Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-099

    authors: Golubev WI,Sampaio JP,Alves L,Golubev NW

    更新日期:2004-12-01 00:00:00

  • Effect of foliar disease on the epiphytic yeast communities of creeping bentgrass and tall fescue.

    abstract::The effect of mechanical wounding or foliar diseases caused by Sclerotinia homoeocarpa or Rhizoctonia solani on the epiphytic yeast communities on creeping bentgrass and tall fescue were determined by leaf washing and dilution plating. Total yeast communities on healthy bentgrass and tall fescue leaves ranged from 7.9...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-073

    authors: Allen TW,Quayyum HA,Burpee LL,Buck JW

    更新日期:2004-10-01 00:00:00

  • Improved antiserum agar method for the serogroup differentiation of Neisseria meningitidis Y and W135.

    abstract::Rabbit antisera prepared to meningococcal serogroups Y and W135 strains were compared with horse antisera using the antiserum agar method (ASA) for the serogroup identification of Neisseria meningitidis. Thirty-seven group Y strains formed immunoprecipitates with the Y rabbit serum only, whereas the same Y strains for...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-109

    authors: Ashton FE,Ryan A,Diena BB

    更新日期:1980-05-01 00:00:00

  • Immune response and protective efficacy of S9 ribosomal protein of Streptococcus pneumoniae in a model of sepsis.

    abstract::Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-083

    authors: Araújo HF,Campos PC,Camargo DR,Pereira FN,Samuel ML,Oliveira MA,Fortes-Dias CL,Leclercq SY

    更新日期:2012-09-01 00:00:00

  • [Rubella virus. I. Morphology and structural proteins].

    abstract::Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Payment P,Ajdukovic D,Pavilanis V

    更新日期:1975-05-01 00:00:00

  • Subcellular localization of the Streptococcus mutans P1 protein C terminus.

    abstract::To determine the subcellular location of the Streptococcus mutans P1 protein C-terminal anchor, cell envelope fractionation experiments were conducted in combination with Western immunoblotting, using monoclonal antibody MAb 6-8C specific for an epitope that maps near the C terminus of P1 protein and also a polyclonal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Homonylo-McGavin MK,Lee SF,Bowden GH

    更新日期:1999-06-01 00:00:00

  • The occurrence of Candida Albicans in Lake Ontario bathing beaches.

    abstract::There are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. Fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. Through surveys of four bathing beaches on Lake Ontario, it w...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-159

    authors: Sherry JP,Kuchma SR,Dutka BJ

    更新日期:1979-09-01 00:00:00

  • Baculovirus-like particles in the reproductive tracts of female parasitoid wasps.

    abstract::Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-148

    authors: Stoltz DB,Vinson SB,MacKinnon EA

    更新日期:1976-07-01 00:00:00

  • Purification and properties of mercuric reductase from Yersinia enterocolitica 138A14.

    abstract::A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m93-027

    authors: Blaghen M,Vidon DJ,el Kebbaj MS

    更新日期:1993-02-01 00:00:00

  • Evaluation of nitrogenous media components by Plackett-Burman statistical design for beta-d-fructofuranosidase production by Saccharomyces sp. strain GVT263.

    abstract::beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-146

    authors: Venkateshwar M,Chaitanya K,Altaf MD,Hameeda B,Ghopal Reddy M

    更新日期:2009-04-01 00:00:00

  • Dietary fructooligosaccharides alter the cultivable faecal population of rats but do not stimulate the growth of intestinal bifidobacteria.

    abstract::The effect of fructans on the cultivable faecal community of Bio Breeding rats fed diets containing 5% (m/v) food-grade fructooligosaccharide (FOS) was investigated. Culturing of faecal material using chicory inulin as the sole carbohydrate source revealed the presence of a greater diversity of inulin-utilizing bacter...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w06-053

    authors: Gourgue-Jeannot C,Kalmokoff ML,Kheradpir E,Kwan J,Lampi BJ,McAllister M,Brooks SP

    更新日期:2006-10-01 00:00:00

  • A turbidimetric and electron microscopic study of the effects of several parameters on the lysis of Streptococcus faecalis by lysozyme.

    abstract::The lytic effect of lysozyme on Streptococcus faecalis ATCC 9790 was studied by spectrophotometry and electron microscopy and it was found to be highly dependent on the ionic strength of the suspending media and on the ratio lysozyme to bacterial cell mass. When 7.2 X 10(8) bacteria/mL are exposed to 0.4 mg/mL of lyso...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-142

    authors: Carvalho ME,Gonçalves MH,Silva MT

    更新日期:1984-07-01 00:00:00

  • Purification and partial characterization of proline dehydrogenase from Clostridium sporogenes.

    abstract::A proline dehydrogenase which catalyzes the nicotinamide adenine dinucleotide (NAD) dependent oxidation of proline and the NADH-dependent reduction of delta 1-pyrroline 5-carboxylic acid (PCA) was purified from extracts of Clostridium sporogenes. Following purification, only one protein band was found on analytical po...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-147

    authors: Monticello DJ,Costilow RN

    更新日期:1981-09-01 00:00:00

  • Glutaraldehyde reactions with alkaline phosphatase of Pseudomonas aeruginosa.

    abstract::Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified pro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-078

    authors: Day DF,Ingram JM

    更新日期:1981-05-01 00:00:00

  • Virus detection in soils: a comparison of four recovery methods.

    abstract::Among nine eluents tested, 0.5% (w/v) isoelectric casein at pH 9.0 and 0.5% (w/v) non-fat dry milk (pH 9.0) were the most efficient in eluting poliovirus type 1 (Sabin) from Eustis fine sand. However, no significant difference was found between the overall (elution followed by concentration) virus recoveries by non-fa...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-130

    authors: Bitton G,Charles MJ,Farrah SR

    更新日期:1979-08-01 00:00:00

  • Binding of mutagenic pyrolyzates to fractions of intestinal bacterial cells.

    abstract::The binding of mutagenic pyrolyzates to cell fractions from some gram-negative intestinal bacteria and to thermally treated bacterial cells was investigated. 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) were effectively bound by several of the bacterial ce...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-101

    authors: Zhang XB,Ohta Y

    更新日期:1992-07-01 00:00:00

  • Characterization of a plasmid in isolates of Corynebacterium sepedonicum.

    abstract::Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 degrees C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight esti...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-116

    authors: Clark MC,Lawrence CH

    更新日期:1986-08-01 00:00:00

  • A multiple extraction--centrifugation method for the recovery of viruses from waste water treatment plant effluents and sludges.

    abstract::Absorption of 14C-labelled poliovirus-2 to sedimentable solids of primary sludge samples collected from a secondary treatment facility during a 6-month period averaged 94%; for anaerobically digested sludge, 99%. The extent of virus adsorption was influenced by the amount of solids. Maximal adsorption occurred at or a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-254

    authors: Lewis MA,Nath MW,Johnson JC

    更新日期:1983-12-01 00:00:00

  • Aerobic adaptation in yeast. I. Changes in metabolic intermediates during a step-down anaerobic-aerobic transfer.

    abstract::Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-126

    authors: Bruver RM,Ball JS,Tustanoff ER

    更新日期:1975-06-01 00:00:00

  • Microbial degradation of nitrobenzene and mono-nitrophenol by bacteria enriched from municipal activated sludge.

    abstract::Using a mixture of three mono nitrophenols as sole carbon, nitrogen and energy sources, mixed cultures were enriched from municipal activated sludge to degrade both nitrophenols and nitrobenzene. Bacterial growth and degradation rate could be increased by supplementing the medium with 0.1% YE. Microorganisms were isol...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Zhao JS,Ward OP

    更新日期:1999-05-01 00:00:00

  • Effect of amino acid deprivation and chloramphenicol treatment on cell sizes of rel+ and relA- strains of Escherichia coli.

    abstract::The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size di...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-127

    authors: Ishiguro EE,Ramey WD

    更新日期:1978-06-01 00:00:00

  • Origin of the eukaryotic nucleus: eukaryotes and eocytes are genotypically related.

    abstract::The origin of the eukaryotic nucleus is difficult to reconstruct. While eukaryotic organelles (chloroplast, mitochondrion) are eubacterial endosymbionts, the source of nuclear genes has been obscured by multiple nucleotide substitutions. Using evolutionary parsimony, a newly developed rate-invariant treeing algorithm,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-017

    authors: Lake JA

    更新日期:1989-01-01 00:00:00

  • Seroepidemiology of Q fever in Nova Scotia and Prince Edward Island.

    abstract::The prevalence of Coxiella burnetti infection (Q fever) was determined among Nova Scotia (N.S.) and Prince Edward Island (P.E.I.) blood donors by using the complement fixation and microimmunofluorescence (IF) test. The complement fixation and IF antibody tests measured antibody prevalence for the phase II or phase I a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-021

    authors: Marrie TJ,Van Buren J,Faulkner RS,Haldane EV,Williams JC,Kwan C

    更新日期:1984-01-01 00:00:00

  • The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. I. Immunizing properties.

    abstract::The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-022

    authors: Diena BB,Ashton FE,Ryan A,Wallace R

    更新日期:1978-02-01 00:00:00

  • Biodegradation of type II pyrethroids and major degraded products by a newly isolated Acinetobacter sp. strain JN8.

    abstract::A Gram-negative aerobic bacterium, designated as JN8, was isolated from activated sludge and soil in a pesticides factory in China. It was found that JN8 had a high capacity for degrading a broad range of type II pyrethroids and utilizing these pyrethroids as the sole carbon source for cell growth. The degradation rat...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0104

    authors: Jin Z,Guo Q,Zhang Z,Yan T

    更新日期:2014-08-01 00:00:00

  • Inactivation of human adenovirus by sequential disinfection with an alternative ultraviolet technology and monochloramine.

    abstract::In an effort to reduce human exposure to adenoviruses through drinking water, we determined the effectiveness of sequential disinfection with an alternative ultraviolet (UV) technology (medium-pressure (MP) UV) and monochloramine. The results of this study showed that MP UV was much more effective than traditional UV ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-047

    authors: Shin GA,Lee JK

    更新日期:2010-07-01 00:00:00

  • Cyanide degradation by an Escherichia coli strain.

    abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-070

    authors: Figueira MM,Ciminelli VS,de Andrade MC,Linardi VR

    更新日期:1996-05-01 00:00:00

  • Restriction-modification systems in Streptomyces antibioticus.

    abstract::Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains. The alternation of efficienc...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m85-177

    authors: Sànchez J,Barbès C,Hernandez A,de los Reyes Gavilàn CR,Hardisson C

    更新日期:1985-10-01 00:00:00

  • Factors affecting the lytic susceptibility of some marine and terrestrial bacteria.

    abstract::Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-063

    authors: Laddaga RA,MacLeod RA

    更新日期:1982-04-01 00:00:00