Evaluation of nitrogenous media components by Plackett-Burman statistical design for beta-d-fructofuranosidase production by Saccharomyces sp. strain GVT263.

Abstract:

:beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using a high enzyme-producing yeast strain. Plackett-Burman statistical experimental design was applied to evaluate the fermentation medium components. The effects of 10 nitrogen sources were studied in a 16-run experimental design. Beef extract, yeast extract, N-Z-amine, tryptone, meat extract, and ammonium acetate were found to have significant effects on enzyme production. Among these, yeast extract, N-Z-amine, and ammonium acetate were the most significant. A maximum FFase activity of 299.4 U/mL was obtained after a 24 h fermentation period.

journal_name

Can J Microbiol

authors

Venkateshwar M,Chaitanya K,Altaf MD,Hameeda B,Ghopal Reddy M

doi

10.1139/w08-146

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

405-9

issue

4

eissn

0008-4166

issn

1480-3275

pii

w08-146

journal_volume

55

pub_type

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