Abstract：
:Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluated the immunogenicity and the protective efficacy of the S9 ribosomal protein of Streptococcus pneumoniae with the aim of developing a protein-based vaccine in the future. The gene encoding the S9 ribosomal protein was cloned in pET21-a expression vector, and the recombinant S9 protein was used to immunize mice. Significantly higher levels of anti-S9 immunoglobulin G were achieved (with predominance of immunoglobulin G1) in comparison with the control. Antibodies elicited against S. pneumoniae protein extract in rabbit recognized the recombinant S9 protein by Western blot, thus demonstrating its immunogenicity. Moreover, mice immunized with recombinant S9 protein and challenged with a virulent strain of S. pneumoniae presented a significant reduction of bacteremia after 24 h of infection as compared with the control. However, in the S9-immunized mice the onset of death was insignificantly delayed, but all of them died by the fourth day postinfection.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Araújo HF,Campos PC,Camargo DR,Pereira FN,Samuel ML,Oliveira MA,Fortes-Dias CL,Leclercq SYdoi
10.1139/w2012-083subject
Has Abstractpub_date
2012-09-01 00:00:00pages
1055-62issue
9eissn
0008-4166issn
1480-3275journal_volume
58pub_type
杂志文章abstract：:The effects of postirradiation incubation with various chemicals structurally related to pantolactone on the survival of ultraviolet-irradiated Escherichia coli lon and ruv mutants were examined. Certain cyclic compounds with a butyrolactone, tetrahydrofuran, furan, tetrahydropyran, or 2-pyron ring showed substantial ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-254
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-190
更新日期：1981-11-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-024
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-063
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journal_title：Canadian journal of microbiology
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更新日期：2006-02-01 00:00:00
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journal_title：Canadian journal of microbiology
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pub_type： 杂志文章
doi：10.1139/m86-006
更新日期：1986-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-012
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-049
更新日期：1986-03-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/m80-216
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journal_title：Canadian journal of microbiology
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doi：
更新日期：1998-02-01 00:00:00
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pub_type： 杂志文章
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：1975-03-01 00:00:00
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更新日期：1977-02-01 00:00:00
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更新日期：2014-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-030
更新日期：1997-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1139/m79-046
更新日期：1979-03-01 00:00:00
