Structural and antigenic variation of the structural protein VP3 in serotype 1 poliovirus isolated from vaccinees.

Abstract:

:High resolution two-dimensional PAGE was used to analyse protein variation among serotype 1 poliovirus isolates. Viruses isolated from patients with recent histories of vaccination with live attenuated poliovirus were compared with prototype serotype 1 poliovaccine. The nonvaccine Mahoney and Brunenders strains of serotype 1 poliovirus were also analysed. The overall protein profile was conserved but the structural protein VP3 varied in its net charge among the viruses. Eight out of 14 clinical virus isolates had VP3 with a net basic charge identical to serotype 1 polio vaccine, whereas the remaining clinical isolates had an acidic VP3 similar to the nonvaccine type 1 strains. The altered VP3 mobility correlated with a change in antigenicity as determined by monoclonal antibodies directed to the neutralization site located on VP3. The data clearly illustrated the suitability of two-dimensional PAGE in analysing protein mutations in attenuated vaccine virus excreted by vaccinees.

journal_name

Can J Microbiol

authors

Cash P

doi

10.1139/m88-136

subject

Has Abstract

pub_date

1988-06-01 00:00:00

pages

802-6

issue

6

eissn

0008-4166

issn

1480-3275

journal_volume

34

pub_type

杂志文章
  • Effect of Bacoside A on growth and biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa.

    abstract::The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A w...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2016-0365

    authors: Parai D,Islam E,Mitra J,Mukherjee SK

    更新日期:2017-02-01 00:00:00

  • Ultraviolet germicidal irradiation disinfection of Stachybotrys chartarum.

    abstract::The ultraviolet germicidal irradiation (UVGI) dose necessary to inactivate fungal spores on an agar surface and the efficacy of UVGI were determined for cultures of Stachybotrys chartarum (ATCC 208877). This study employed a UVGI testing unit consisting of four chambers with a 9-W, Phillips, low pressure, mercury UVGI...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-061

    authors: Green CF,Davidson CS,Scarpino PV,Gibbs SG

    更新日期:2005-09-01 00:00:00

  • Restriction analysis of actinomycetes chromosomal DNA.

    abstract::Actinomycetes DNAs were digested with restriction enzymes to study the presence of methylated bases. Analysis showed that the enterobacterial Dam and Dcm systems are absent. Methylation at the internal cytosine in CCGG sequences, typical of eukaryotes, was also absent. We also tested 18 restriction endonucleases recog...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-031

    authors: Novella IS,Marín I,Sánchez J

    更新日期:1996-02-01 00:00:00

  • Heterokaryosis and parasexuality in Pyricularia oryzae Cavara.

    abstract::Auxotrophs in three different races of Pyricularia oryzae were obtained following ultraviolet mutagenesis, and tested for complementarity on minimal medium. Prototrophic growth resulted between combinations of different auxotrophs of single strains, but not between strains. The growth did not result form crossfeeding,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-079

    authors: Genovesi AD,Magill CW

    更新日期:1976-04-01 00:00:00

  • The microbial degradation of cyclohexanecarboxylic acid by a beta-oxidation pathway with simultaneous induction to the utilization of benzoate.

    abstract::The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-141

    authors: Blakley ER

    更新日期:1978-07-01 00:00:00

  • Purification and some properties of the citrate synthase from a marine Pseudomonas.

    abstract::Citrate synthase (citrate-oxaloacetate lyase (CoA acetylating), EC 4.1.3.7) has been purified to electrophoretic homogeneity from a marine Pseudomonas. The enzyme was made up of identical subunits, with a molecular wieght of about 53 000, as determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis. T...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-039

    authors: Higa AI,Massarini E,Cazzulo JJ

    更新日期:1978-03-01 00:00:00

  • Antioxidant, antihypertensive, and antibacterial properties of endophytic Pestalotiopsis species from medicinal plants.

    abstract::Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-070

    authors: Tejesvi MV,Kini KR,Prakash HS,Subbiah V,Shetty HS

    更新日期:2008-09-01 00:00:00

  • The use of T1 oligonucleotide mapping to determine that the emergence of Canadian 1973-like and 1974-like H3N2 strains in 1984 was the result of laboratory contamination.

    abstract::Influenza undergoes extensive antigenic variation in nature. These new antigenic variants invariably supplant the preceding antigenic type that then disappears. In apparent contrast to this, two H3N2 strains that were forwarded to the Laboratory Centre for Disease Control, Ottawa, in 1984 from a Canadian public health...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m87-139

    authors: Brown EG

    更新日期:1987-09-01 00:00:00

  • Characterization of 4 T1-like lytic bacteriophages that lyse Shiga-toxin Escherichia coli O157:H7.

    abstract::Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-063

    authors: Niu YD,Stanford K,Ackermann HW,McAllister TA

    更新日期:2012-07-01 00:00:00

  • Study of characteristics on metabolism of Penicillium chrysogenum F1 during bioleaching of heavy metals from contaminated soil.

    abstract::Penicillium chrysogenum F1 is very efficient in bioleaching heavy metals from the soil and is used for that purpose. We found that F1 can extract 19.8 mg Cd, Cu, Pb, and Zn from 2.5 g soil; the total heavy metals' bioleaching ratio was 60.4%. In this study, the bioleaching mechanism was investigated by means of metabo...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0624

    authors: Deng X,Yang Z,Chen R

    更新日期:2019-09-01 00:00:00

  • Host cell invasion and intracellular residence by Aeromonas salmonicida: role of the S-layer.

    abstract::Virulent strains of the fish pathogen Aeromonas salmonicida, which have surface S-layers (S+), efficiently adhere to, enter, and survive within macrophages. Here we report that S+ bacteria were 10- to 20-fold more adherent to non-phagocytic fish cell lines than S-layer-negative (S-) mutants. When reconstituted with ex...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w00-034

    authors: Garduño RA,Moore AR,Olivier G,Lizama AL,Garduño E,Kay WW

    更新日期:2000-07-01 00:00:00

  • Selection of genetically diverse Trichoderma spp. isolates for suppression of Phytophthora capsici on bell pepper.

    abstract::Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, G...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-071

    authors: Roberts DP,Maul JE,McKenna LF,Emche SE,Meyer SL,Collins RT,Bowers JH

    更新日期:2010-10-01 00:00:00

  • Metal binding by the peptidoglycan sacculus of Escherichia coli K-12.

    abstract::The peptidoglycan of Escherichia coli K-12 strain AB264 was isolated by treating whole cells with sodium dodecyl sulfate and was purified by deoxyribonuclease, ribonuclease, and trypsin treatment. Like the peptidoglycan of Bacillus subtilis, this peptidoglycan proved able to bind substantial amounts of metallic ions f...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-031

    authors: Hoyle BD,Beveridge TJ

    更新日期:1984-02-01 00:00:00

  • Menadione reductase from Clostridium tyrobutyricum.

    abstract::Menadione reductase (EC 1.6.99.2) has been purified 67-fold from Clostridium tyrobutyricum extracts. The molecular weight was found to be 60 000 and the prosthetic group was identified as FMN on the basis of the enzymatic analysis data. The binding of FMN to the menadione dehydrogenase apoenzyme was relatively weak wi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-053

    authors: Petitdemange H,Marczak R,Raval G,Gay R

    更新日期:1980-03-01 00:00:00

  • Bacterial evolution.

    abstract::The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章,评审

    doi:10.1139/m88-093

    authors: Doolittle WF

    更新日期:1988-04-01 00:00:00

  • Internalization of Mycobacterium shottsii and Mycobacterium pseudoshottsii by Acanthamoeba polyphaga.

    abstract::Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0079

    authors: Gupta T,Fine-Coulson K,Karls R,Gauthier D,Quinn F

    更新日期:2013-08-01 00:00:00

  • A N2-fixing endophytic Burkholderia sp. associated with maize plants cultivated in Mexico.

    abstract::In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied. Under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-023

    authors: Estrada P,Mavingui P,Cournoyer B,Fontaine F,Balandreau J,Caballero-Mellado J

    更新日期:2002-04-01 00:00:00

  • Serotype distribution of invasive Streptococcus pneumoniae in Canada after the introduction of the 13-valent pneumococcal conjugate vaccine, 2010-2012.

    abstract::The introduction of the 7-valent pneumococcal vaccine (PCV7) in Canada was very effective in reducing invasive pneumococcal disease (IPD) in children; however, increases of non-PCV7 serotypes have subsequently offset some of these reductions. A 13-valent pneumococcal vaccine (PCV13) targeting additional serotypes was ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0614

    authors: Demczuk WH,Martin I,Griffith A,Lefebvre B,McGeer A,Lovgren M,Tyrrell GJ,Desai S,Sherrard L,Adam H,Gilmour M,Zhanel GG,Toronto Bacterial Diseases Network.,Canadian Public Health Laboratory Network.

    更新日期:2013-12-01 00:00:00

  • Complete genome sequence of Paracoccus denitrificans ATCC 19367 and its denitrification characteristics.

    abstract::Studies show that Paracoccus denitrificans can denitrify nitrogen sources under aerobic conditions. However, the lack of data on its genome sequence has restricted molecular studies and practical applications. In this study, the complete genome of P. denitrificans ATCC 19367 was sequenced and its nitrogen metabolism p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2019-0037

    authors: Si YY,Xu KH,Yu XY,Wang MF,Chen XH

    更新日期:2019-07-01 00:00:00

  • Effect of environmental characteristics on Pythium and Mesocriconema spp. in golf course greens in Alabama.

    abstract::The role the environment has on populations of Pythium and Mesocriconema spp. was investigated at 5 golf course locations in east central Alabama. Every 4 to 5 weeks soil samples were collected from 3 golf greens on each of the 5 golf courses. Environmental data, including air and soil temperature, pH and relative hum...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-005

    authors: Allen TW,Han DY,Bowen KL

    更新日期:2005-04-01 00:00:00

  • Morphological modifications of cells of Clostridium pasteurianum caused by growth on sulfite.

    abstract::The morphology of Clostridium pasteurianum cells grown on 10(-2) M SO32- showed significant alteration in cell shape and the absence of the electron translucent reserve polysaccharide (amylopectin) when compared to sulfate-grown cells. At the lower sulfite concentrations (10(-3) and 10(-4)M SO32-) the cells showed the...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-036

    authors: McCready RG,Costerton JW,Laishley EJ

    更新日期:1976-02-01 00:00:00

  • Rv3351c, a Mycobacterium tuberculosis gene that affects bacterial growth and alveolar epithelial cell viability.

    abstract::Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0528

    authors: Pavlicek RL,Fine-Coulson K,Gupta T,Quinn FD,Posey JE,Willby M,Castro-Garza J,Karls RK

    更新日期:2015-12-01 00:00:00

  • Molecular and morphological markers for rapid distinction between 2 Colletotrichum species.

    abstract::Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-059

    authors: Pileggi SA,Vieira de Oliveira SF,Andrade CW,Vicente VA,Dalzoto Pdo R,Kniphoff da Cruz G,Gabardo J,Massola NS,Tozze HJ,Pileggi M,Kava-Cordeiro V,Galli-Terasawa LV,Pimentel IC,Stringari D,Glienke C

    更新日期:2009-09-01 00:00:00

  • Isolation and PCR amplification of a species-specific oxidoreductase-coding gene region in Listeria grayi.

    abstract::Listeria grayi is a nonpathogenic Gram-positive bacterium that demonstrates considerable similarities to other members in the genus Listeria, including the foodborne human pathogen Listeria monocytogenes and the animal pathogen Listeria ivanovii. A rapid diagnostic test to identify and diagnose listeriosis would be va...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-108

    authors: Liu D,Lawrence ML,Ainsworth AJ,Austin FW

    更新日期:2005-01-01 00:00:00

  • Aged-related physiological studies comparing Candida albicans chlamydospores to yeasts.

    abstract::From electron-microscopical observations, a decreased metabolic activity in 3-day-old Candida albicans chlamydospores was suggested, and progressive deterioration in chlamydospores aged 2-8 months was shown. Oxygen utilization by chlamydospore-pseudomycelium (CSP) preparations was less than that by yeast, while 3-day-...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-111

    authors: Miller SE,Finnerty WR

    更新日期:1979-06-01 00:00:00

  • Denitrifying bacteria in bulk and maize-rhizospheric soil: diversity and N2O-reducing abilities.

    abstract::The aim of this study was to determine the effect of the rhizosphere of maize on the diversity of denitrifying bacteria. Community structure comparison was performed by constructing a collection of isolates recovered from bulk and maize planted soil. A total of 3240 nitrate-reducing isolates were obtained and 188 of t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-037

    authors: Chèneby D,Perrez S,Devroe C,Hallet S,Couton Y,Bizouard F,Iuretig G,Germon JC,Philippot L

    更新日期:2004-07-01 00:00:00

  • The presence of 3-hydroxy oxylipins on the ascospore surfaces of some species representing Saccharomycopsis Schiönning.

    abstract::Through gas chromatography - mass spectrometry, the presence of oxylipins, mainly 3-hydroxy 9:1 and 3-hydroxy 10:1, was detected in Saccharomycopsis fermentans, Saccharomycopsis javanensis, and Saccharomycopsis vini. The distribution of these compounds was mapped using immunofluorescence microscopy, and they were foun...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-037

    authors: Sebolai OM,Kock JL,Pohl CH,Botes PJ,Strauss CJ,Van Wyk PW,Nigam S

    更新日期:2005-07-01 00:00:00

  • Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants.

    abstract::Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-015

    authors: Pfeifer F,Blaseio U,Horne M

    更新日期:1989-01-01 00:00:00

  • Bioflotation of sulfide minerals with Acidithiobacillus ferrooxidans in relation to copper activation and surface oxidation.

    abstract::Surface oxidation of sulfides and copper (Cu) activation are 2 of the main processes that determine the efficiency of flotation. The present study was developed with the intention to ascertain the role of the phenomena in the biomodification of sulfides by Acidithiobacillus ferrooxidans culture (cells and growth me...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-072

    authors: Pecina-Treviño ET,Ramos-Escobedo GT,Gallegos-Acevedo PM,López-Saucedo FJ,Orrantia-Borunda E

    更新日期:2012-09-01 00:00:00

  • Symbiosis of selected Rhizobium leguminosarum bv. viciae strains with diverse pea genotypes: effects on biological nitrogen fixation.

    abstract::Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate the super-nodulating pea mutant...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2017-0281

    authors: Yang C,Bueckert R,Schoenau J,Diederichsen A,Zakeri H,Warkentin T

    更新日期:2017-11-01 00:00:00