Abstract：
:The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A were tested using the minimum inhibitory concentration and minimum bactericidal concentration assays. A cell membrane disruption assay was performed to find its possible target site. MTT assay, crystal violet assay, and microscopic studies were performed to assess the antibiofilm activity. Bacoside A showed antimicrobial activity against both test organisms in their planktonic and biofilm states. At a subminimum inhibitory concentration of 200 μg·mL-1, Bacoside A significantly removed ∼88%-93% of bacterial biofilm developed on microtiter plates. Biochemical and microscopic studies suggested that the eradication of biofilm might be due to the loss of extracellular polymeric substances and to a change in cell membrane integrity of the selected bacterial strains treated with Bacoside A. These results indicate that Bacoside A might be considered as an antimicrobial having the ability to disrupt biofilms. Thus, either alone or in combination with other therapeutics, Bacoside A could be useful to treat biofilm-related infections caused by opportunistic bacterial pathogens.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Parai D,Islam E,Mitra J,Mukherjee SKdoi
10.1139/cjm-2016-0365subject
Has Abstractpub_date
2017-02-01 00:00:00pages
169-178issue
2eissn
0008-4166issn
1480-3275journal_volume
63pub_type
杂志文章abstract：:In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of ...
journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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更新日期：2000-08-01 00:00:00
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doi：10.1139/m83-177
更新日期：1983-09-01 00:00:00
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journal_title：Canadian journal of microbiology
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更新日期：2000-12-01 00:00:00
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更新日期：1977-03-01 00:00:00
abstract：:Giardia duodenalis is a common intestinal parasite in most parts of the world. In Canada it is associated with both endemic and epidemic infections that are often transmitted by the waterborne route. Although G. duodenalis strains have been isolated from several animals, the role of other mammals in human infection is...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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