Abstract：
:A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virology, such as rapid screening of samples for virus content or assay of neutralizing antibodies, is also discussed.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Campbell JB,Grunberger T,Kochman MA,White SLdoi
10.1139/m75-186subject
Has Abstractpub_date
1975-08-01 00:00:00pages
1247-53issue
8eissn
0008-4166issn
1480-3275journal_volume
21pub_type
杂志文章abstract：:Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be compos...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-105
更新日期：1979-06-01 00:00:00
abstract：:The lytic effect of lysozyme on Streptococcus faecalis ATCC 9790 was studied by spectrophotometry and electron microscopy and it was found to be highly dependent on the ionic strength of the suspending media and on the ratio lysozyme to bacterial cell mass. When 7.2 X 10(8) bacteria/mL are exposed to 0.4 mg/mL of lyso...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-142
更新日期：1984-07-01 00:00:00
abstract：:Using a mixture of three mono nitrophenols as sole carbon, nitrogen and energy sources, mixed cultures were enriched from municipal activated sludge to degrade both nitrophenols and nitrobenzene. Bacterial growth and degradation rate could be increased by supplementing the medium with 0.1% YE. Microorganisms were isol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m95-091
更新日期：1995-08-01 00:00:00
abstract：:Responses of Rhizoctonia solani to herbicides in soil cultures were assessed by measuring soil enzyme activity and other growth-related factors. Both beta-galactosidase (EC 3.2.1.23) and phosphatase (EC 3.1.3.1.3.1.3.2) activities were highly correlated with amounts of mycelium in soil. Both enzyme activities were red...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-089
更新日期：1977-05-01 00:00:00
abstract：:The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-157
更新日期：1991-12-01 00:00:00
abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-063
更新日期：1979-03-01 00:00:00
abstract：:BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to ind...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0057
更新日期：2016-09-01 00:00:00
abstract：:Yersinia enterocolitica biotype 1A (B1A) strains are considered mainly nonpathogenic. However, some studies considered strains of this biotype to be the causal agents of infections in humans and animals. In South America, there are no studies that have compared clinical and nonclinical strains of B1A typed by pulsed-f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0211
更新日期：2014-06-01 00:00:00
abstract：:Fluorescent Pseudomonas spp. isolated from fruiting bodies (FB) of Cantharellus cibarius were characterized physiologically and genetically and were compared with fluorescent Pseudomonas from forest soil and with sequences from the GenBank database. Pseudomonas spp. from FB differed physiologically from isolates from ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-062
更新日期：2002-08-01 00:00:00
abstract：:In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Urinary tract infections are common. Few published studies have demonstrated the change in Escherichia coli urinary isolate antimicrobial susceptibility over time within a given area and (or) population. The purpose of this study was to evaluate the change in susceptibility of E. coli clinical isolates obtained from u...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-001
更新日期：2012-03-01 00:00:00
abstract：:Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Four phages isolated from cattle and poultry feces were analyzed for their ability to lyse Salmonella serotypes and Escherichia coli O157:H7. The phage one-step growth curves, morphology, and genetic characteristics were determined. All phages showed a lytic effect on various Salmonella serotypes and E. coli O157:H7, ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-099
更新日期：2011-12-01 00:00:00
abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-018
更新日期：2009-06-01 00:00:00
abstract：:Pili are essential adhesive determinants for many bacterial pathogens. A suppressor mutation screen that takes advantage of a pilus-mediated self-aggregative "hockey-puck" colony phenotype was designed to identify novel regulators of type I pili in Serratia marcescens. Mutations that decreased pilus biosynthesis mappe...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0566
更新日期：2016-01-01 00:00:00
abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-102
更新日期：2007-01-01 00:00:00
abstract：:Verticillium fungicola, isolated from Agaricus bisporus (commercial mushroom), produced significant extracellular hydrophobin when grown for 7 days in a static liquid culture of synthetic minimal medium. The hydrophobin was purified by precipitation with ammonium sulphate (80% saturation), Sephadex G-100 gel filtratio...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-098
更新日期：2002-11-01 00:00:00
abstract：:The catalytic subunit of many Escherichia coli redox enzymes bares a twin-arginine translocation (Tat)-dependent signal peptide in its precursor, which directs the redox enzyme complex to this Sec-independent pathway. NarG of the E. coli nitrate reductase NarGHI complex possesses a vestige twin-arginine motif at its N...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-111
更新日期：2009-02-01 00:00:00
abstract：:The physiological characteristics and fatty acid composition of 40 strains of Yarrowia lipolytica isolated from irradiated poultry meat, commercial chilled foods, the superficial water of lagoons of an Italian river delta, and commercial light butter were compared. Discriminant analysis of the results permitted the 40...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-008
更新日期：1994-01-01 00:00:00
abstract：:This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-001
更新日期：1983-01-01 00:00:00
abstract：:Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-002
更新日期：2007-04-01 00:00:00
abstract：:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-131
更新日期：2005-03-01 00:00:00
abstract：:The use of microorganisms for bioremediation of contaminated soils may be enhanced with an understanding of the pathways involved in their degradation of hazardous compounds. Ralstonia sp. strain RJGII.123 was isolated from soil located at a former coal gasification plant, based on its ability to mineralize carbazole,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-131
更新日期：2000-03-01 00:00:00
abstract：:Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-085
更新日期：2005-07-01 00:00:00
abstract：:Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial scree...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-043
更新日期：2006-09-01 00:00:00
abstract：:The shikimate metabolites are key precursors to a large number of natural products, including aromatic amino acids. Chorismic acid is an important branch point in the biosynthetic pathway to aromatic amino acids. Chorismic acid is also unique among natural products since it is the only compound known to undergo an enz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-043
更新日期：1991-04-01 00:00:00
abstract：:A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate deter...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
abstract：:The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the st...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-290
更新日期：1975-12-01 00:00:00
abstract：:The respiration of Candida lipolytica on n-tetradecane is decreased in the presence of bentonite. This inhibition is less pronounced by the introduction of hydrophobic chains on the bentonite. Oxygen demand of resting cells varies with the length of hydrophobic chains. In fermentor, addition of adsorbant does not stim...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
