Abstract：
:Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial screening step to identify strains for heavy metal decontamination in food and intestinal models. Binding isotherms for cadmium and lead were characterized for Lactobacillus rhamnosus LC-705, Propionibacterium freudenreichii subsp. shermanii JS and a mix of them used by the food industry. Differences among the strains and their combinations in binding performance at a range of concentrations between 0.1 and 100 mg.L-1 were evaluated with the Langmuir model for biosorption. The effects of pH, contact time, and viability on the binding capacities were also investigated. All strains and their combinations were found to bind cadmium and lead efficiently at low concentration ranges commonly observed in foods. However, the two strains and their combinations differed significantly in their maximum binding capacities and affinities represented by the Langmuir constants Qmax and b, respectively. The binding seemed to occur instantaneously and in a pH-dependent manner, which can be perfectly described by a segmented linear-plateau model.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ibrahim F,Halttunen T,Tahvonen R,Salminen Sdoi
10.1139/w06-043subject
Has Abstractpub_date
2006-09-01 00:00:00pages
877-85issue
9eissn
0008-4166issn
1480-3275pii
w06-043journal_volume
52pub_type
杂志文章abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-102
更新日期：2007-01-01 00:00:00
abstract：:Leptospira interrogans serotype pomona was isolated from the kidneys of a normal striped skunk (Mephitis mephitis hudsonicus) collected near Kindersley, Saskatchewan. Although there were no gross lesions, small foci of chronic interstitial nephritis were observed microscopically. This is the first reported isolation o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-237
更新日期：1977-12-01 00:00:00
abstract：:The gene coding for glycoprotein B2 (gB2) of herpes simplex virus type 2 (HSV-2) strain 333 was mapped and its nucleotide sequence determined. Open reading frame analysis deduced a polypeptide consisting of 902 amino acids and having close homology to gB1 of HSV type 1. Several predicted features of gB2 are consistent...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-154
更新日期：1987-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-075
更新日期：1991-06-01 00:00:00
abstract：:Colletotrichum gloeosporioides is the causal agent of rubber tree anthracnose and leads to serious losses of natural rubber production. The pathogenesis of C. gloeosporioides on rubber tree remains unknown. Cerato-platanin proteins are small, secreted cysteine-rich proteins that contribute to virulence and function in...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0087
更新日期：2018-11-01 00:00:00
abstract：:The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W06-124
更新日期：2007-03-01 00:00:00
abstract：:In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:A virulent strain of pigeon pea wilt pathogen was isolated from wilted pigeon pea plants and was identified as Fusarium oxysporum f. sp. udum. Many bacterial cultures showing antagonism to the pathogen were isolated from various ecological niches. When tested under pot and field conditions, development of fusarial wil...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-109
更新日期：2000-02-01 00:00:00
abstract：:The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-155
更新日期：1996-12-01 00:00:00
abstract：:A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-186
更新日期：1975-08-01 00:00:00
abstract：:The ultraviolet germicidal irradiation (UVGI) dose necessary to inactivate fungal spores on an agar surface and the efficacy of UVGI were determined for cultures of Stachybotrys chartarum (ATCC 208877). This study employed a UVGI testing unit consisting of four chambers with a 9-W, Phillips, low pressure, mercury UVGI...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-061
更新日期：2005-09-01 00:00:00
abstract：:Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified pro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-078
更新日期：1981-05-01 00:00:00
abstract：:Histological examination of oesophagus and stomach specimens from 28 guinea pigs (Cavia porcellus) revealed microbes associated with the epithelial surface of these organs. Layers of Gram-positive cocci were associated with oesophageal tissue in all the animals. Yeasts (Torulop-sid pintolopesii) were associated with s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-182
更新日期：1978-09-01 00:00:00
abstract：:A bacterium C1010, isolated from the rhizospheres of cucumbers in fields in Korea, degraded the microbial quorum-sensing molecules, hexanoyl homoserine lactone (HHSL), and octadecanoyl homoserine lactone (OHSL). Morphological characteristics and 16S rRNA sequence analysis identified C1010 as Acinetobacter sp. strain C...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-083
更新日期：2004-11-01 00:00:00
abstract：:An anaerobic consortium that carboxylated and dehydroxylated phenol to benzoate, and 2-cresol to 3-methylbenzoic acid, under methanogenic conditions was studied. Phenol induced this transformation activity. Addition of 4-hydroxypyridine or an increase in the concentration of proteose peptone to 0.5% (w/v) delayed the ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-093
更新日期：1993-07-01 00:00:00
abstract：:The production of cold shock proteins (csps) and cold acclimation proteins (caps) was characterized in the psychrotrophic bacterium Pseudomonas putida Q5 and its transconjugant P. putida Q5T which contains the toluene-degradative TOL (pWWO) plasmid, using two-dimensional gel electrophoresis and computing scanning lase...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-100
更新日期：1996-08-01 00:00:00
abstract：:The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2017-0585
更新日期：2018-03-01 00:00:00
abstract：:Nitrogenase in Azotobacter chroococcum whole cells was inhibited by enzymically generated superoxide anion (O2-), hydrogen peroxide, and ethyl hydrogen peroxide. The degree of inhibition produced by O2- was related to the quantity of oxygen supplied to the organisms in continuous cultures. O2- also inhibited oxygen up...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-228
更新日期：1977-11-01 00:00:00
abstract：:A series of synthetic promoters, based upon the Escherichia coli sigma 70 consensus promoter sequence, was constructed upstream of the lacZ reporter gene in the modified broad-host-range vector pQF52. The role of the intervening spacer region in gene expression in Pseudomonas aeruginosa and E. coli was studied by inse...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-141
更新日期：1997-10-01 00:00:00
abstract：:The presence of killer and proteolytic yeasts was studied among 944 isolates representing 105 species from tropical yeast communities. We found 13 killer toxin producing species, with Pichia kluyveri being the most frequent. Other killer yeast isolates were Candida apis, Candida bombicola, Candida fructus, Candida kru...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-046
更新日期：1997-04-01 00:00:00
abstract：:Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-004
更新日期：2003-01-01 00:00:00
abstract：:Eight Enterococcus faecium strains isolated from ewe milk and artisanal cheese from northwest Argentina were screened for biotechnological properties relevant to flavour development. The API ZYM test showed absence of proteases, presence of high amounts of peptidases, and high esterase-lipase activities. Low extracell...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-136
更新日期：2006-03-01 00:00:00
abstract：:Fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of Clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. Enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. With the development of the spore, enterotoxin accumula...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-134
更新日期：1977-07-01 00:00:00
abstract：:Frankia are gram-positive, filamentous bacteria capable of fixing atmospheric dinitrogen in symbiosis with a wide variety of woody plants and shrubs. Some isolates of Frankia harbor plasmids of 8.5 (pFQ11) and 22.4 kb (pFQ12) that have no known function but are transmitted through many generations in culture. We have ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-050
更新日期：2001-07-01 00:00:00
abstract：:This study examined the phylogenetic structure of serotype a Haemophilus influenzae (Hia) isolates recovered from patients in Canada. Hia isolates from 490 separate patients and an American Type Culture Collection (ATCC) strain were analyzed by multilocus sequence typing (MLST), with 18 different sequence types (STs) ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0406
更新日期：2020-02-01 00:00:00
abstract：:Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-227
更新日期：1976-10-01 00:00:00
abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-129
更新日期：2002-01-01 00:00:00
abstract：:Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-100
更新日期：1989-06-01 00:00:00
abstract：:The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-022
更新日期：1978-02-01 00:00:00
