Abstract:
:Nitrogenase in Azotobacter chroococcum whole cells was inhibited by enzymically generated superoxide anion (O2-), hydrogen peroxide, and ethyl hydrogen peroxide. The degree of inhibition produced by O2- was related to the quantity of oxygen supplied to the organisms in continuous cultures. O2- also inhibited oxygen uptake by whole cells. These O2- mediated inhibitions were prevented by bovine superoxide dismutase. The quantities of superoxide dismutase (SOD), and catalase associated with cells grown under varying oxygen concentrations were determined. The role of hydrogen peroxide, and of the hydroxyl radical (.OH) in nitrogenase inhibition was examined. The response of Azotobacter chroococum to oxygen was evaluated with respect to the observed effects of O2- on the organism, and some explanation is given to account for nitrogenase sensitivity to oxygen.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Buchanan AGdoi
10.1139/m77-228subject
Has Abstractpub_date
1977-11-01 00:00:00pages
1548-53issue
11eissn
0008-4166issn
1480-3275journal_volume
23pub_type
杂志文章abstract::Responses of Rhizoctonia solani to herbicides in soil cultures were assessed by measuring soil enzyme activity and other growth-related factors. Both beta-galactosidase (EC 3.2.1.23) and phosphatase (EC 3.1.3.1.3.1.3.2) activities were highly correlated with amounts of mycelium in soil. Both enzyme activities were red...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-089
更新日期:1977-05-01 00:00:00
abstract::The correlation between the occurrence of indicator organisms and salmonellae in the same sample homogenate of various classes of dried foods was studied. In the first phase of the work involving 5837 samples, 1943 contained fecal coliforms and 100 contained Salmonella. Fecal coliforms were not detected in 53 of the S...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-141
更新日期:1976-07-01 00:00:00
abstract::Trichloroethylene (TCE) is a prevalent contaminant of groundwater that can be cometabolically degraded by indigenous microbes. Groundwater contaminated with TCE from a US Department of Energy site in Ohio was used to characterize the site-specific impact of phenol on the indigenous bacterial community for use as a pos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-025
更新日期:2005-06-01 00:00:00
abstract::The effect of a chromated-copper-arsenate wood preservative on the degradation of pentachlorophenol by Flavobacterium sp. strain ATCC 53874 was examined in liquid culture. Both a commercially available and a laboratory-prepared formulation were tested. Each increased the lag time required for measurable pentachlorophe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-063
更新日期:1994-05-01 00:00:00
abstract::The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::Alkaline phosphatase (APase) was present in the bovine rumen in both cell-free and cell-associated states and levels of the enzyme varied with dietary regime. Reaction product deposition showed that the enzyme was associated with the mixed bacterial population. No enzyme was observed to be associated with protozoa. Tr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-111
更新日期:1976-05-01 00:00:00
abstract::The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly diffe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-081
更新日期:2009-10-01 00:00:00
abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0399
更新日期:2014-10-01 00:00:00
abstract::The aim of this study was to unravel, by focusing on cell surface properties, the underlying virulence factors contributing to the difference in the pathogenicity observed in two Acinetobacter baumannii strains isolated from the same patient. The two strains were phenotypically different: (i) a mucoid strain (AB-M),...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-131
更新日期:2012-03-01 00:00:00
abstract::We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N', N'-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-040
更新日期:2005-08-01 00:00:00
abstract::Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-062
更新日期:2006-10-01 00:00:00
abstract::Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-073
更新日期:1997-06-01 00:00:00
abstract::Five media and procedures were compared for their recovery of fecal streptococci on membrane filters. Growth and numbers of a variety of pure cultures were compared, and recoveries from raw sewage and sewage effluent were determined on the five media. A total of 512 isolates were identified and the specificity of each...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-219
更新日期:1980-11-01 00:00:00
abstract::Bacillus stearothermophilus NCA 2184 lost viability and subsequently released cytoplasmic components when suspended in 0.1 M tris(hydroxymethyl)aminomethane (Tris) buffer (pH 7.2) and incubated at 60 degrees C. Cell lysis was prevented by the addition of 10 mM CaCl2 to the Tris-buffer suspension. Cells which were incu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-073
更新日期:1976-04-01 00:00:00
abstract::Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-102
更新日期:1990-08-01 00:00:00
abstract::Group B Neisseria meningitidis (SD1C) was grown on defined medium supplemented with each of a variety of sulphur compounds as the sole source of sulphur. The organism grew on sulphate, sulphite, bisulphite, thiosulphate, dithionite, hydrosulphide, thiocyanate, L-cysteine, L-cystine, reduced glutathione, methionine, me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-232
更新日期:1984-12-01 00:00:00
abstract::Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-083
更新日期:1979-05-01 00:00:00
abstract::A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w99-055
更新日期:1999-08-01 00:00:00
abstract::Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0703
更新日期:2016-07-01 00:00:00
abstract::The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-090
更新日期:1990-07-01 00:00:00
abstract::Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
abstract::Natural populations of bacteria assoiciated with the slime on submerged surfaces in a mountain stream were examined by phase-contrast and electron microscopy. The slime contained large numbers of bacteria which were predominantly gram-negative as determined by their cell wall structure. Examination of the in situ dist...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-249
更新日期:1977-12-01 00:00:00
abstract::Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-063
更新日期:1982-04-01 00:00:00
abstract::To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg x L(-1) ciprofloxacin. After the cultures had been centrifuged and the sup...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-101
更新日期:2007-01-01 00:00:00
abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-227
更新日期:1976-10-01 00:00:00
abstract::The synergistic interactions between Pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (MCMV) were compared in vivo. Virulent MCMV challenge at a dose of 5 X 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 X 10(6) cfu/mouse of Pseudomonas aeruginosa after 48 h res...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-161
更新日期:1987-10-01 00:00:00
abstract::Passive protective activities of three different classes of monoclonal antibodies in mice against challenge with strain ATCC 31432 (capsular type I) of Staphylococcus epidermidis were examined. Monoclonal IgM antibody passively protected mice against challenge with the homologous strain, whereas monoclonal IgG1 and Ig...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-065
更新日期:1991-05-01 00:00:00
abstract::Using a gene fusion approach, hybrid proteins were created by linking alkaline phosphatase (PhoA) or a cellulase reporter (delta CenA) to four large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein (RsaA; 1026 amino acids). Three of the sites (amino acids 189, 220, 315) were selected on the ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-122
更新日期:1994-09-01 00:00:00
abstract::A study has been undertaken to account for the presence of Thiobacillus acidophilus in iron-grown cultures of Thiobacillus ferrooxidans. Attempts to adapt T. acidophilus to ferrous iron were not successful but the facultative autotroph grew to a limited extent in the spent medium of T. ferrooxidans and was able to gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-178
更新日期:1980-09-01 00:00:00
abstract::Bacteria are known to rapidly produce new phenotypes, but it is unclear how phenotype "plasticity" relates to studies on the population ecology of bacteria in complex environments. We characterised a collection of 14 spontaneous phenotype variants, derived from in vitro and in vivo cultures (wheat roots) of Pseudomona...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w99-006
更新日期:1999-04-01 00:00:00