The response of Azotobacter chroococcum to oxygen: superoxide-mediated effects.

Abstract:

:Nitrogenase in Azotobacter chroococcum whole cells was inhibited by enzymically generated superoxide anion (O2-), hydrogen peroxide, and ethyl hydrogen peroxide. The degree of inhibition produced by O2- was related to the quantity of oxygen supplied to the organisms in continuous cultures. O2- also inhibited oxygen uptake by whole cells. These O2- mediated inhibitions were prevented by bovine superoxide dismutase. The quantities of superoxide dismutase (SOD), and catalase associated with cells grown under varying oxygen concentrations were determined. The role of hydrogen peroxide, and of the hydroxyl radical (.OH) in nitrogenase inhibition was examined. The response of Azotobacter chroococum to oxygen was evaluated with respect to the observed effects of O2- on the organism, and some explanation is given to account for nitrogenase sensitivity to oxygen.

journal_name

Can J Microbiol

authors

Buchanan AG

doi

10.1139/m77-228

subject

Has Abstract

pub_date

1977-11-01 00:00:00

pages

1548-53

issue

11

eissn

0008-4166

issn

1480-3275

journal_volume

23

pub_type

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