Abstract:
:Five media and procedures were compared for their recovery of fecal streptococci on membrane filters. Growth and numbers of a variety of pure cultures were compared, and recoveries from raw sewage and sewage effluent were determined on the five media. A total of 512 isolates were identified and the specificity of each medium was determined from these results. While KE agar recovered significantly higher numbers of organisms, only 81% of the positive colonies were fecal streptococci. Slanetz and Bartley (SB) medium showed similarly low specificity and the mE procedure gave low recoveries. Best overall results were obtained on mEnterococcus (mEnt) agar with high (91%) specificity for enterococci, and very good recovery efficiency with low background counts. This medium was also the only one demonstrating any appreciable recovery of other fecal streptococci with 93% of the background colonies being confirmed as fecal streptococci which most were Streptococcus bovis, S. equinus, or S. salivarius. Pfizer selective enterococcus (PSE) agar was second in overall performance with a high (94%) rate of specificity but this medium had lower recovery efficiency and high background counts. Performance of PSE agar could be considerably improved, without impairing specificity, by using a total count disregarding esculin hydrolysis.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Pagel JE,Hardy GMdoi
10.1139/m80-219subject
Has Abstractpub_date
1980-11-01 00:00:00pages
1320-7issue
11eissn
0008-4166issn
1480-3275journal_volume
26pub_type
杂志文章abstract::We report a cinematographic analysis of culmination in fruiting bodies of Polysphondylium pallidum. During the final stages of aggregation, sorogen elongation is slow. An increase in the rate of sorogen elongation occurs when the growing stalk contacts the substratum, lifting the cell mass into the air. The elongation...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-163
更新日期:1980-08-01 00:00:00
abstract::Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris et P. morganii tryptophanases (TPases) were studied for the spectral forms of the enzyme. The pH effect on the absorption spectrum and on the enzyme specific activity revealed that the coli group TPases are identical with but differ from P...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
abstract::An obligate methyltroph Methylomonas methylovora oxidized methylamine, formaldehyde, and formate. Enzymes oxidizing these substrates were detected in a cell-free system. Phenazine methosulfate-linked methylamine dehydrogenase was purified 21-fold. The enzyme had optimum activity at pH 7.5 and was stable at 60 degrees ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-059
更新日期:1977-04-01 00:00:00
abstract::To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0755
更新日期:2014-03-01 00:00:00
abstract::The monitoring of antimicrobial susceptibilities in Neisseria gonorrhoeae isolates and characterization of N. gonorrhoeae multiantigen sequence types (NG-MAST, ST) provide important surveillance data as resistance rates continue to rise. A total of 2970 N. gonorrhoeae isolates were collected by Canadian provincial pub...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0357
更新日期:2013-10-01 00:00:00
abstract::Streptomyces echinatus A8331 cultured on a maltose minimal salts medium normally produces a single antibiotic, echinomycin (quinomycin A), containing two quinoxaline-2-carbonyl chromophores. Echinomycin is powerfully active against experimental tumours and can be assayed by its activity against Gram-positive bacteria....
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-065
更新日期:1984-04-01 00:00:00
abstract::Microsclerotia of three melanin-deficient mutants of Verticillium dahliae formed malanin from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. The melanins formed from (+)-scytalone or 1,8-dihydroxynaphthalene resembled wild-type melanin chemically and ultrastructurally, whereas the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-049
更新日期:1978-03-01 00:00:00
abstract::In an effort to reduce human exposure to adenoviruses through drinking water, we determined the effectiveness of sequential disinfection with an alternative ultraviolet (UV) technology (medium-pressure (MP) UV) and monochloramine. The results of this study showed that MP UV was much more effective than traditional UV ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-047
更新日期:2010-07-01 00:00:00
abstract::Giardia duodenalis is a common intestinal parasite in most parts of the world. In Canada it is associated with both endemic and epidemic infections that are often transmitted by the waterborne route. Although G. duodenalis strains have been isolated from several animals, the role of other mammals in human infection is...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-171
更新日期:1986-12-01 00:00:00
abstract::Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these ogranisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. This organism requires salts of seawater for both growth and extracell...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-213
更新日期:1976-10-01 00:00:00
abstract::The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-239
更新日期:1976-11-01 00:00:00
abstract::In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-186
更新日期:1988-09-01 00:00:00
abstract::Highly distinctive aspects of the exponentail-phase Rhizobium japonicum cell were disclosed by means of thin sections, freeze etching, fluorescent antibodies, and ruthenium red staining. Polarity was expressed in the form of reserve polymer distribution near one end of the cell and as cytoplasmic localization near the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-191
更新日期:1977-09-01 00:00:00
abstract::A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-162
更新日期:1997-12-01 00:00:00
abstract::The acetylene-reduction assay was used for in situ and laboratory assessment of biological nitrogen fixation in the acidic, waterlogged, muskeg ecosystem of the southern James Bay area, in the region of Moosonee, Ontario. In situ assays and subsequent laboratory experimentation revealed that nitrogenase activity was p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-130
更新日期:1976-07-01 00:00:00
abstract::The effects of selected culture conditions on production of Shiga-like toxin-II variant by an edema disease strain of Escherichia coli (412) and E. coli TB1 (pCG6) containing the cloned genes for Shiga-like toxin-II variant were examined. Incubation time, culture media, incubation temperature, starting pH of the cultu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-100
更新日期:1989-06-01 00:00:00
abstract::Citrobacter freundii from an environmental source displayed resistance to a wide variety of antibacterials. Some of these resistances could be transferred by conjugation with frequencies as high as 10(-2). The complexity of plasmid involvement in the antibacterial resistance of this species was demonstrated by isolati...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-052
更新日期:1981-03-01 00:00:00
abstract::Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-deriv...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0485
更新日期:2014-12-01 00:00:00
abstract::The search for new antimicrobial compounds involves finding novel sources of chemotherapeutic compounds or manipulating and combining structures from existing molecules. Small antimicrobial peptides (AMPs) are components of innate immune defenses characterized in greatest detail in insect-derived AMPs. We have generat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-001
更新日期:2009-05-01 00:00:00
abstract::Methanospirillum hungatei strains GP1 and JF1 when exposed to the adenosinetriphosphatase inhibitor N,N'-dicyclohexylcarbodiimide experienced a marked decline in growth rates, methane synthesis activities, and intracellular ATP concentrations. Although growth was inhibited, the intracellular ATP concentrations of all ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-146
更新日期:1982-08-01 00:00:00
abstract::The effect of atmospheric composition and storage temperature on growth and survival of uninjured and sublethally heat-injured Escherichia coli O157:H7, inoculated onto brain heart infusion agar containing 0.3% beef extract (BEM), was determined. BEM plates were packaged in barrier bags in air, 100% CO2, 100% N2, 20% ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w98-224
更新日期:1999-03-01 00:00:00
abstract::A large pool of antibiotic resistant and auxotrophic mutants was isolated from the Staphylococcus aureus phage group 2 strains UT0002-19 and UT0017 by (1) antibiotic gradient plates, (2) trimethoprim selection, and (3) nitrosoguanidine mutagenesis, which sometimes was coupled by enrichment with either penicillin or me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-161
更新日期:1980-08-01 00:00:00
abstract::Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-099
更新日期:1991-08-01 00:00:00
abstract::Campylobacter spp. are a substantial cause of gastroenteritis worldwide. Human infection can result from ingestion of contaminated food or water from a variety of sources, including the consumption of fresh produce that is contaminated with the pathogen via the use of contaminated irrigation water. Using molecular met...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0710
更新日期:2016-05-01 00:00:00
abstract::Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a mon...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0155
更新日期:2019-10-01 00:00:00
abstract::Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-079
更新日期:1978-04-01 00:00:00
abstract::Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-164
更新日期:1984-08-01 00:00:00
abstract::The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to q...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::An investigation of brucellosis caused by Brucella suis, type 4, in reindeer, Rangifer tarandus L., and other ungulates inoculated experimentally with virulent isolates was undertaken to observe the course of infection, follow titres of serum agglutins, and determine the extent to which intraspecific and interspecific...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-024
更新日期:1978-02-01 00:00:00
abstract::Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-065
更新日期:1976-03-01 00:00:00