Abstract:
:Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The virus increased more than 100-fold in the experimentally infected mosquitoes. The increasing viral titres were noticed after 7 days and after 15 days. Peak titres averaged 105.0 (mean suckling mouse intracerebral lethal dose) SMICLD50/0.02 mL. The infected mosquitoes had peak titres until at least 35 days after the mosquitoes ingested blood from infected suckling mice. A single trasmission of virus by bite occurred 30 days after the viraemic blood meal. Transovarial transmission was demonstrated. In two experiments, 3.3 and 2.9% of infected mosquitoes transovarially transmitted Cache Valley virus to both male and female progeny. The minimum infection rate for the progeny was 2.05/1000 mosquitoes. This is the first reported experimental demonstration of transovarian transmission in a species of mosquito which overwinters as an adult. The role of transovarian transmission in the natural maintenance of Cache Valley virus remains undetermined.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Hayles LB,Lversen JOsubject
Has Abstractpub_date
1980-03-01 00:00:00pages
287-90issue
3eissn
0008-4166issn
1480-3275journal_volume
26pub_type
杂志文章abstract::We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-059
更新日期:1988-03-01 00:00:00
abstract::This study, the first of arbovirus activity in Prince Edward Island, has shown antibodies to the snowshoe hare strain of the California encephalitis group virus to be present in sera of wild and domestic animals; 15.35% of snowshoe hare, 20% of equine. and 2.5% of bovine sera were positive for hemagglutination inhibit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-188
更新日期:1981-11-01 00:00:00
abstract::Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-041
更新日期:1978-03-01 00:00:00
abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0399
更新日期:2014-10-01 00:00:00
abstract::The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concent...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-021
更新日期:2007-05-01 00:00:00
abstract::Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-105
更新日期:2007-12-01 00:00:00
abstract::Dithiothreitol reacted, at pH 9.0, with the isolated cell walls of Methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). Untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. Sugars were identified as rhamnose,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-017
更新日期:1980-02-01 00:00:00
abstract::To assess the effect of fire and salvage logging on the diversity of mycorrhizal-bacterial communities, bacteria associated with Cenococcum, Thelephora, Tomentella, Russulaceae, and E-strain ectomycorrhizae (ECM) of Abies lasiocarpa seedlings were characterized using two approaches. First, bacteria were isolated and c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-056
更新日期:2002-07-01 00:00:00
abstract::Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-245
更新日期:1976-11-01 00:00:00
abstract::A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacteria...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-018
更新日期:2003-02-01 00:00:00
abstract::Candida tropicalis synthesizes a hydroxylase (3 to 5 nmol of product formed per minute per milligram of protein) and a cytochrome P-450 (0.10 to 0.13 nmol per milligram of protein) during growth on n-tetradecane. A three- to four-fold increase in the level of NADPH cytochrome c reductase is also observed in those cell...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-031
更新日期:1979-02-01 00:00:00
abstract::Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0343
更新日期:2020-10-01 00:00:00
abstract::Streptomyces echinatus A8331 cultured on a maltose minimal salts medium normally produces a single antibiotic, echinomycin (quinomycin A), containing two quinoxaline-2-carbonyl chromophores. Echinomycin is powerfully active against experimental tumours and can be assayed by its activity against Gram-positive bacteria....
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-065
更新日期:1984-04-01 00:00:00
abstract::Pili are essential adhesive determinants for many bacterial pathogens. A suppressor mutation screen that takes advantage of a pilus-mediated self-aggregative "hockey-puck" colony phenotype was designed to identify novel regulators of type I pili in Serratia marcescens. Mutations that decreased pilus biosynthesis mappe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0566
更新日期:2016-01-01 00:00:00
abstract::Three composts (Ball, dairy, and greenhouse) were tested for the ability to suppress the development of Fusarium root and stem rot (caused by Fusarium oxysporum f. sp. radicis-cucumerinum) on greenhouse cucumber. Dairy and greenhouse composts significantly reduced disease severity (P = 0.05), while Ball compost had no...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-076
更新日期:2010-11-01 00:00:00
abstract::The effect of phenolic compounds, ethyl alcohol, and sodium metabisulphite on the lytic activity of virulent bacteriophage PL-1 on a Lactobacillus casei S strain isolated from a lactic acid beverage fermentation was investigated. Catechin, caffeic, and gallic acids, commercially produced red, white, and champagne tann...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-162
更新日期:1985-09-01 00:00:00
abstract::Urinary tract infections are common. Few published studies have demonstrated the change in Escherichia coli urinary isolate antimicrobial susceptibility over time within a given area and (or) population. The purpose of this study was to evaluate the change in susceptibility of E. coli clinical isolates obtained from u...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-001
更新日期:2012-03-01 00:00:00
abstract::Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-136
更新日期:1990-11-01 00:00:00
abstract::Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-102
更新日期:1990-08-01 00:00:00
abstract::We modified a technique, cDNA-AFLP, for identifying differentially expressed genes in plants to work in the filamentous fungus Leptosphaeria maculans (Desmaz.) Ces. & De Not. The cDNA fragments generated by our method ranged in size from approximately 100 to 400 bps. On average, twice as many cDNA fragments were ampli...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-100
更新日期:2001-10-01 00:00:00
abstract::The types of bacteria and fungi present in the air of a municipal solid waste recovery system have been characterized and the population densities estimated. Conventional methods were successful in enumerating coagulase-positive staphylococci, Klebsiella spp., gram-negative bacteria, fungi, and common indicator organi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-038
更新日期:1985-03-01 00:00:00
abstract::Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-067
更新日期:2005-10-01 00:00:00
abstract::Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-093
更新日期:2009-11-01 00:00:00
abstract::During shadowing, a "cap" of metal develops on small particles. This cap increases apparent particle with (measured normal to the shadowing direction) by an extent which cannot be predetermined. The extent of this increase in particle size (here defined as the "cap," X) is estimated in the present method by using oppo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-114
更新日期:1985-07-01 00:00:00
abstract::Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-062
更新日期:2006-10-01 00:00:00
abstract::The physiochemical characteristics of the intracellular proteolytic enzymes of Oidiodendron kalari, a neuropathogenic fungus, were studied. The organism in the yeast phase was grown in a semisynthetic medium containing 1% tryptone, at 37 degrees C for 48 hr, on a gyrotory shaker. The crude extract was prepared by brea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-204
更新日期:1975-09-01 00:00:00
abstract::We examined factors related to the potent antagonistic effect of Escherichia coli and Bacteroides ovatus on Staphylococcus aureus in anaerobic continuous flow cultures. In the presence of sugars fermentable by E. coli alone or both E. coli and S. aureus, motile E. coli strains exerted a potent antagonistic effect and ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-106
更新日期:1988-05-01 00:00:00
abstract::Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-004
更新日期:2003-01-01 00:00:00
abstract::Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-015
更新日期:1989-01-01 00:00:00