Abstract：
:We examined factors related to the potent antagonistic effect of Escherichia coli and Bacteroides ovatus on Staphylococcus aureus in anaerobic continuous flow cultures. In the presence of sugars fermentable by E. coli alone or both E. coli and S. aureus, motile E. coli strains exerted a potent antagonistic effect and S. aureus was expelled from the culture vessel within a few days. Conversely, in the presence of a sugar fermentable by S. aureus alone, the antagonistic effect of E. coli was diminished and S. aureus persisted at ca. 5 x 10(5) cfu/mL. B. ovatus alone exerted only a weak antagonistic effect on S. aureus in any culture conditions; however, when B. ovatus was cocultivated with E. coli and S. aureus, even in the presence of a sugar fermentable by S. aureus but not by E. coli, the potent antagonistic effect was restored. Escherichia coli showed the same level of antagonistic effect either in the presence of acetic acid (ca. 32 mM), propionic acid (4 mM), butyric acid (17 mM) and hydrogen sulfide (5 x 10(-1) mM) or when these metabolic products, except for a small amount of acetic acid (1.2 mM) were not present. In these culture conditions, S. aureus populations were lost at rates much higher than theoretical wash out rates of resting cells. These results indicate the presence of some bactericidal factors other than the volatile fatty acids and hydrogen sulfide. The bactericidal factors were not found in cultures of E. coli heated in boiling water for 10 min and in cell-free culture filtrates. Thus, the bactericidal factors seem to be associated with live E. coli cells. The nature of the bactericidal factors is not clear at present.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ushijima T,Ozaki Ydoi
10.1139/m88-106subject
Has Abstractpub_date
1988-05-01 00:00:00pages
645-50issue
5eissn
0008-4166issn
1480-3275journal_volume
34pub_type
杂志文章abstract：:A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophag...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-030
更新日期：1997-03-01 00:00:00
abstract：:Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris et P. morganii tryptophanases (TPases) were studied for the spectral forms of the enzyme. The pH effect on the absorption spectrum and on the enzyme specific activity revealed that the coli group TPases are identical with but differ from P...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
abstract：:In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied. Under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-023
更新日期：2002-04-01 00:00:00
abstract：:To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0755
更新日期：2014-03-01 00:00:00
abstract：:The purpose of this study was to evaluate experimentally the immunogenicity in rabbits of rubella subunits adsorbed to the adjuvant Quil A. The adsorbed viral proteins form structurally defined ImmunoStimulating COMplexes (ISCOMs). Rubella ISCOMs were tested for their capacity to induce neutralizing and hemagglutinati...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-238
更新日期：1988-12-01 00:00:00
abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-018
更新日期：2009-06-01 00:00:00
abstract：:Highly distinctive aspects of the exponentail-phase Rhizobium japonicum cell were disclosed by means of thin sections, freeze etching, fluorescent antibodies, and ruthenium red staining. Polarity was expressed in the form of reserve polymer distribution near one end of the cell and as cytoplasmic localization near the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-191
更新日期：1977-09-01 00:00:00
abstract：:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-151
更新日期：1979-09-01 00:00:00
abstract：:New antibiotics produced by Streptomyces echinatus A8331 cultured in the presence of heterocyclic aromatic acids can be separated and purified by high-performance liquid chromatography using reversed phase columns. Natural quinoxaline antibiotics and certain quinoline derivatives can also be efficiently separated in n...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-112
更新日期：1984-06-01 00:00:00
abstract：:The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-119
更新日期：1990-10-01 00:00:00
abstract：:A previously unmapped pan gene was localized in an Escherichia coli K-12 strain using Hfr and F' matings and by transduction studies. This Pan- mutation was shown by supplementation studies to impart a block in pantoate biosynthesis. The pan gene cotransduced with rha, metB, and argE placing it at 87 min. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-190
更新日期：1981-11-01 00:00:00
abstract：:The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m88-093
更新日期：1988-04-01 00:00:00
abstract：:Two rod-shaped viruses of similar length occurring in hops were isolated from a mixed infection. One (hop mosaic virus) infected Nicotiana clevelandii, produced mosaic symptoms in mosaic-sensitive hop cultivars, and did not infect Chenopodium murale. The other (hop latent virus) infected C. murale but not N. cleveland...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-168
更新日期：1976-08-01 00:00:00
abstract：:Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-041
更新日期：1978-03-01 00:00:00
abstract：:A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-106
更新日期：1983-06-01 00:00:00
abstract：:Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W10-087
更新日期：2010-12-01 00:00:00
abstract：:Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-119
更新日期：2005-02-01 00:00:00
abstract：:From the four known isoforms of the staphylococcal exfoliative toxins (ETs), only ETA and ETB are the major causative agents. General knowledge is that the gene for ETA is located on the chromosome, whereas that for ETB is located on a large plasmid. Yoshizawa and co-workers (2000, Microbiol. Immunol. 44(3): 189-191) ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0721
更新日期：2013-04-01 00:00:00
abstract：:During shadowing, a "cap" of metal develops on small particles. This cap increases apparent particle with (measured normal to the shadowing direction) by an extent which cannot be predetermined. The extent of this increase in particle size (here defined as the "cap," X) is estimated in the present method by using oppo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-114
更新日期：1985-07-01 00:00:00
abstract：:Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-209
更新日期：1983-10-01 00:00:00
abstract：:Effects of Trichoderma harzianum and T. polysporum on chlamydospore production by two isolates of Phytophthora cinamomi were determined over a 21-day period in nonsterile, sterilized, and sterilized amended soil. Trichoderma was either coinoculated with P. cinnamomi or added to the cultures on day 3 of the incubation ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-042
更新日期：1977-03-01 00:00:00
abstract：:Bacteria are known to rapidly produce new phenotypes, but it is unclear how phenotype "plasticity" relates to studies on the population ecology of bacteria in complex environments. We characterised a collection of 14 spontaneous phenotype variants, derived from in vitro and in vivo cultures (wheat roots) of Pseudomona...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-006
更新日期：1999-04-01 00:00:00
abstract：:A moderately halophilic Acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. Maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2M NaCl or 1M KCl. Two kinds of amylase, amylases I and II were purified from the culture fil...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-169
更新日期：1978-09-01 00:00:00
abstract：:Total community DNA from 29 noncontaminated soils and soils impacted by petroleum hydrocarbons and chloro-organics from Antarctica and Brazil were screened for the presence of nine catabolic genes, encoding alkane monooxygenase or aromatic dioxygenases, from known bacterial biodegradation pathways. Specific primers an...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-008
更新日期：2004-05-01 00:00:00
abstract：:In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Products obtained from the action of arylamine synthetase on [G-14C]chorismic acid were fractionated by gel filtration and ion exchange column chromatography to yield a partially purified radioactive component with an arylamine function. From its ultraviolet absorption spectrum and thin-layer chromatographic behaviour...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-032
更新日期：1976-02-01 00:00:00
abstract：:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-095
更新日期：1986-06-01 00:00:00
abstract：:Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/w2012-025
更新日期：2012-05-01 00:00:00
abstract：:Mutants of Trichoderma harzianum with altered antibiotic production were isolated using ultraviolet light mutagenesis. These included strains whose activity in a Fusarium oxysporum spore germination assay was greater than twice that of the parental strain and one that had no detectable antifungal activity. Characteris...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-112
更新日期：1991-09-01 00:00:00
abstract：:An endo-beta-1,4-xylanase gene was cloned from Neocallimastix patriciarum 27 in the bacteriophage vector lambda gtWES lambda B and was subcloned into the plasmid vectors pUC18 and pUC19 in which xylanase activity was expressed in both orientations. The xylanase was located in the periplasmic space of the host, Escheri...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-020
更新日期：1993-01-01 00:00:00
