Biosynthesis of chloramphenicol in Streptomyces sp. 3022a. Identification of p-amino-L-phenylalanine as a product from the action of arylamine synthetase on chorismic acid.

Abstract:

:Products obtained from the action of arylamine synthetase on [G-14C]chorismic acid were fractionated by gel filtration and ion exchange column chromatography to yield a partially purified radioactive component with an arylamine function. From its ultraviolet absorption spectrum and thin-layer chromatographic behaviour the product was considered to be p-aminophenylalanine and the identification was confirmed by co-crystallization with an authentic specimen. Specific deamination of the product with L-amino-acid oxidase indicated that it was the L-epimer. These results strengthen previous evidence that arylamine synthetase is at a branch point in the shikimic acid pathway, specifically diverting intermediates to the synthesis of chloramphenicol.

journal_name

Can J Microbiol

authors

Jones A,Vining LC

doi

10.1139/m76-032

subject

Has Abstract

pub_date

1976-02-01 00:00:00

pages

237-44

issue

2

eissn

0008-4166

issn

1480-3275

journal_volume

22

pub_type

杂志文章
  • Mechanism of boron tolerance in soil bacteria.

    abstract::Boron (B) is toxic to living cells at levels above a certain threshold. We isolated several B-tolerant bacterial strains from soil samples and studied them for possible mechanisms of B tolerance. 16S rRNA gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 gen...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-106

    authors: Ahmed I,Fujiwara T

    更新日期:2010-01-01 00:00:00

  • Effect of Bacoside A on growth and biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa.

    abstract::The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A w...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2016-0365

    authors: Parai D,Islam E,Mitra J,Mukherjee SK

    更新日期:2017-02-01 00:00:00

  • Binding of the Bacillus sphaericus mosquito larvicidal toxin to cultured insect cells.

    abstract::Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m87-173

    authors: Davidson EW,Shellabarger C,Meyer M,Bieber AL

    更新日期:1987-11-01 00:00:00

  • Bactericidal activity of magainin 2: use of lipopolysaccharide mutants.

    abstract::Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m90-102

    authors: Macias EA,Rana F,Blazyk J,Modrzakowski MC

    更新日期:1990-08-01 00:00:00

  • Aerobic adaptation in yeast. I. Changes in metabolic intermediates during a step-down anaerobic-aerobic transfer.

    abstract::Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-126

    authors: Bruver RM,Ball JS,Tustanoff ER

    更新日期:1975-06-01 00:00:00

  • Bacteriophage lambda repressor allelic modulation of the Rex exclusion phenotype.

    abstract::The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-re...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-021

    authors: Slavcev RA,Bull HJ,Hayes S

    更新日期:2003-03-01 00:00:00

  • Cytokinin production by plant growth promoting rhizobacteria and selected mutants.

    abstract::One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w01-029

    authors: García de Salamone IE,Hynes RK,Nelson LM

    更新日期:2001-05-01 00:00:00

  • Ultrastructural effects of lysozymes on the cell wall of Caryophanon latum.

    abstract::When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the s...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-024

    authors: Trentini WC,Murray RG

    更新日期:1975-02-01 00:00:00

  • Oxygen toxicity in Treponema pallidum: deoxyribonucleic acid single-stranded breakage induced by low doses of hydrogen peroxide.

    abstract::The effect of hydrogen peroxide on Treponema pallidum was investigated. The in vitro loss of virulence (as measured by rabbit inoculation) of T. pallidum was accelerated by as low as 100 microM hydrogen peroxide in the complex maintenance medium used. Higher doses led to rapidly accelerated death with 500 microM hydro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-234

    authors: Steiner BM,Wong GH,Sutrave P,Graves S

    更新日期:1984-12-01 00:00:00

  • Swarming motility by Photorhabdus temperata is influenced by environmental conditions and uses the same flagella as that used in swimming motility.

    abstract::Photorhabdus temperata, an insect pathogen and nematode symbiont, is motile in liquid medium by swimming. We found that P. temperata was capable of surface movement, termed swarming behavior. Several lines of evidence indicate that P. temperata use the same flagella for both swimming and swarming motility. Both motili...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W10-119

    authors: Michaels B,Tisa LS

    更新日期:2011-03-01 00:00:00

  • The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuary.

    abstract::The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cyto...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2012-0606

    authors: Pereira Md,Byrne BA,Nguyen TB,Lewis DJ,Atwill ER

    更新日期:2013-06-01 00:00:00

  • Aminoglycoside resistance mechanisms in Pseudomonas aeruginosa isolates from non-cystic fibrosis patients in Thailand.

    abstract::This study aimed to examine aminoglycosides (AMGs) resistance mechanisms, including the AMG-modifying enzyme genes, mexXY, rplY, nuoG, and galU, in the Pseudomonas aeruginosa non-cystic fibrosis (CF) isolates in Thailand. One hundred P. aeruginosa isolates from non-CF patients were examined for susceptibility to AMGs ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2012-0465

    authors: Poonsuk K,Tribuddharat C,Chuanchuen R

    更新日期:2013-01-01 00:00:00

  • Improving the culturability of freshwater bacteria using FW70, a low-nutrient solid medium amended with sodium pyruvate.

    abstract::Bacterial culture based on the use of plate media is an effective method for investigating bacterial populations in the environment. To improve the culturability of bacteria from freshwater lakes, we developed a new medium, FW70, which contains sodium pyruvate, casamino acids, and lake water and is solidified using ge...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-019

    authors: Imazaki I,Kobori Y

    更新日期:2010-04-01 00:00:00

  • Genotyping of Yersinia enterocolitica biotype 1A strains from clinical and nonclinical origins by pulsed-field gel electrophoresis.

    abstract::Yersinia enterocolitica biotype 1A (B1A) strains are considered mainly nonpathogenic. However, some studies considered strains of this biotype to be the causal agents of infections in humans and animals. In South America, there are no studies that have compared clinical and nonclinical strains of B1A typed by pulsed-f...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0211

    authors: Campioni F,Falcão JP

    更新日期:2014-06-01 00:00:00

  • Accumulation of radiocaesium in fungi.

    abstract::The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m90-119

    authors: Bakken LR,Olsen RA

    更新日期:1990-10-01 00:00:00

  • Studies on experimental murine histoplasmosis: host protection and cellular immunity.

    abstract::Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by q...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-016

    authors: Burt WR,Smith RA

    更新日期:1983-01-01 00:00:00

  • Antigenic analysis of Pasteurella multocida (serotype 1) by crossed immunoelectrophoresis: characterization of cytoplasmic and cell envelope associated antigens.

    abstract::The application of crossed immunoelectrophoresis to the analysis of a reference cytoplasm and cell envelope preparation from Pasteurella multocida serotype 1 revealed antigenic complexity not previously found. At least 55 cytoplasmic and 19 cell envelope antigens were clearly distinguished. Variation of anticytoplasm ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-118

    authors: Bhasin JL,Lapointe-Shaw L

    更新日期:1980-06-01 00:00:00

  • Inhibition of growth, iron, and sulfur oxidation in Thiobacillus ferrooxidans by simple organic compounds.

    abstract::Iron and sulfur oxidation by Thiobacillus ferrooxidans as well as growth on ferrous iron were inhibited by a variety of low molecular weight organic compounds. The influences of chemical structure of the organic inhibitors, pH, temperature, physical treatment of cells, and added inhibitory or stimulatory inorganic ion...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-105

    authors: Tuttle JH,Dugan PR

    更新日期:1976-05-01 00:00:00

  • Thymineless death in Escherichia coli mutants deficient in the RecF recombination pathway.

    abstract::Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-157

    authors: Nakayama K,Shiota S,Nakayama H

    更新日期:1988-07-01 00:00:00

  • Ecology of cultivable yeasts in pristine forests in northern Patagonia (Argentina) influenced by different environmental factors.

    abstract::Environmental factors influencing the occurrence and community structure of soil yeasts in forests are not well studied. There are few studies dedicated to Southern Hemisphere soil yeasts populations and even fewer focused on temperate forests influenced by volcanic activity. The present work aimed to study the ecolog...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0897

    authors: Mestre MC,Fontenla S,Rosa CA

    更新日期:2014-06-01 00:00:00

  • Sensitivity of methanogenic bacteria to dicyclohexylcarbodiimide.

    abstract::Methanospirillum hungatei strains GP1 and JF1 when exposed to the adenosinetriphosphatase inhibitor N,N'-dicyclohexylcarbodiimide experienced a marked decline in growth rates, methane synthesis activities, and intracellular ATP concentrations. Although growth was inhibited, the intracellular ATP concentrations of all ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-146

    authors: Sprott GD,Jarrell KF

    更新日期:1982-08-01 00:00:00

  • Analysis of biofilm formation by intestinal lactobacilli.

    abstract::In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0007

    authors: Slížová M,Nemcová R,Mad'ar M,Hadryová J,Gancarčíková S,Popper M,Pistl J

    更新日期:2015-06-01 00:00:00

  • Lower dietary concentrate level increases bacterial diversity in the rumen of Cervus elaphus yarkandensis.

    abstract::The ruminal microbiota plays major roles in feed digestion. The composition and fermentation of the bacterial communities in 3 important ruminant species have been studied previously. Here, we extended this research to the effect of concentrate-to-forage ratios on ruminal bacterial communities in Tarim red deer (Cervu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0046

    authors: Qian W,Ao W,Hui X,Wu J

    更新日期:2018-07-01 00:00:00

  • Discriminant analysis of microcalorimetric data of bacterial growth.

    abstract::In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-186

    authors: Bermúdez J,López D,Sanahuja A,Viñas M,Lorén JG

    更新日期:1988-09-01 00:00:00

  • Attempts to inhibit ruminal methanogenesis by blocking pyruvate oxidative decarboxylation.

    abstract::The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-079

    authors: Ungerfeld EM,Rust SR,Burnett R

    更新日期:2003-10-01 00:00:00

  • The nucleotide sequence of herpes simplex virus type 2 (333) glycoprotein gB2 and analysis of predicted antigenic sites.

    abstract::The gene coding for glycoprotein B2 (gB2) of herpes simplex virus type 2 (HSV-2) strain 333 was mapped and its nucleotide sequence determined. Open reading frame analysis deduced a polypeptide consisting of 902 amino acids and having close homology to gB1 of HSV type 1. Several predicted features of gB2 are consistent...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m87-154

    authors: Zwaagstra JC,Leung WC

    更新日期:1987-10-01 00:00:00

  • [Increase of resistance to Pseudomonas aeruginosa infection in mice caused by Staphylococcus aureus].

    abstract::In our study of opportunistic pathogens, we have some indication that Staphylococcus aureus can increase resistance in mice against Pseudomonas aeruginosa. Intraperitoneal injections of sublethal doses of S. aureus had a protective effect in mice against lethal doses of P. aeruginosa, more so if living and coagulase-p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: deRepentigny J,Mathieu LG,Coallier-Ascah J

    更新日期:1976-07-01 00:00:00

  • Characterization of diacetin B, a bacteriocin from Lactococcus lactis subsp. lactis bv. diacetylactis UL720.

    abstract::Fourteen Lactococcus lactis strains showing inhibitory activity against Listeria innocua SICC 4202 were isolated from different French raw milks and raw milk cheeses and screened for bacteriocin production by the triple layer method under conditions that eliminate the effects of lactic acid and hydrogen peroxide. Thre...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m95-114

    authors: Ali D,Lacroix C,Thuault D,Bourgeois CM,Simard RE

    更新日期:1995-09-01 00:00:00

  • Soil microbial communities of three grassland ecosystems in the Bayinbuluke, China.

    abstract::The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2017-0585

    authors: Shao K,Gao G

    更新日期:2018-03-01 00:00:00

  • Microbial community structure and trichloroethylene degradation in groundwater.

    abstract::Trichloroethylene (TCE) is a prevalent contaminant of groundwater that can be cometabolically degraded by indigenous microbes. Groundwater contaminated with TCE from a US Department of Energy site in Ohio was used to characterize the site-specific impact of phenol on the indigenous bacterial community for use as a pos...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-025

    authors: Humphries JA,Ashe AM,Smiley JA,Johnston CG

    更新日期:2005-06-01 00:00:00