Abstract:
:Products obtained from the action of arylamine synthetase on [G-14C]chorismic acid were fractionated by gel filtration and ion exchange column chromatography to yield a partially purified radioactive component with an arylamine function. From its ultraviolet absorption spectrum and thin-layer chromatographic behaviour the product was considered to be p-aminophenylalanine and the identification was confirmed by co-crystallization with an authentic specimen. Specific deamination of the product with L-amino-acid oxidase indicated that it was the L-epimer. These results strengthen previous evidence that arylamine synthetase is at a branch point in the shikimic acid pathway, specifically diverting intermediates to the synthesis of chloramphenicol.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Jones A,Vining LCdoi
10.1139/m76-032subject
Has Abstractpub_date
1976-02-01 00:00:00pages
237-44issue
2eissn
0008-4166issn
1480-3275journal_volume
22pub_type
杂志文章abstract::Metarhizium anisopliae isolates have a wide insect host range, but an impediment to their commercial use as a biocontrol agent of above-ground insects is the high susceptibility of spores to the near-UV present in solar irradiation. To understand stress responses in M. anisopliae, we initiated studies of enzymes that ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-097
更新日期:2004-01-01 00:00:00
abstract::Ribosomal preparations from Neisseria gonorrhoeae types 1 and 4 were examined for their in vitro stimulation of mouse splenocytes to determine the ribosomal moiety or contaminant responsible for the immunoproliferative activity. In immunodiffusion tests with homologous rabbit antiserum, crude 70S ribosomes formed four...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-094
更新日期:1978-05-01 00:00:00
abstract::In Salmonella typhimurium LT2 the F factor of Escherichia coli K-12 replicates normally but is repressed; Flac+ cells give no visible lysis on solid media with male-specific phages, low frequency transfer of Flac+ (0.001-0.007 per donor cell), few f2 infective centers (0.002-0.006 per cell), and they propagate male-sp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-184
更新日期:1983-09-01 00:00:00
abstract::The effect of the transition metal compound trans-[Rh(4-ethylpyridine)4Cl2]Cl x 2H2O on the syntheses of DNA, RNA, and protein has been investigated for an auxotrophic bacterial strain, Escherichia coli JS-1, incapable of thymidine, uridine, and histidine syntheses. At low concentration (7.4 x 10(-6) M), this rhodium ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-111
更新日期:1980-05-01 00:00:00
abstract::Chloroquine increases the inhibition of cultured lymphocytes by high concentrations of phytohemagglutinin (PHA) or concanavalin A (Con A). The inhibition is also increased by complement. Thus chloroquine and complement have similar effects. Time-course studies show that chloroquine increases the rate of onset of the c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-231
更新日期:1975-10-01 00:00:00
abstract::Nodulation of Pisum sativum cv. Afghanistan by Rhizobium leguminosarum strain Tom can be blocked by the nonnodulating R. leguminosarum strain PF2. This system was used to study the dynamics of rhizobial binding to seedling root surfaces. Appreciable numbers of 35S-labeled rhizobia bound to the root surface within 2h a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-099
更新日期:1980-04-01 00:00:00
abstract::The nucleotide sequence of the murE gene encoding the diaminopimelic acid adding enzyme of Escherichia coli is reported. The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs. The deduced primary structure of MurE comprised 471 amino acid re...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-175
更新日期:1989-11-01 00:00:00
abstract::Eight Enterococcus faecium strains isolated from ewe milk and artisanal cheese from northwest Argentina were screened for biotechnological properties relevant to flavour development. The API ZYM test showed absence of proteases, presence of high amounts of peptidases, and high esterase-lipase activities. Low extracell...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-136
更新日期:2006-03-01 00:00:00
abstract::Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-024
更新日期:1980-02-01 00:00:00
abstract::The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-120
更新日期:1979-07-01 00:00:00
abstract::Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-201
更新日期:1980-10-01 00:00:00
abstract::Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-083
更新日期:2005-11-01 00:00:00
abstract::Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-153
更新日期:1978-08-01 00:00:00
abstract::Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-083
更新日期:1979-05-01 00:00:00
abstract::A test is described for selective inhibition of Mycobacterium tuberculosis and M. bovis isolates in fluid medium. The method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (NAP) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other Mycobacteria. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-124
更新日期:1978-06-01 00:00:00
abstract::Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-239
更新日期:1975-10-01 00:00:00
abstract::Seven streptomycete strains were tested for biotransformation of salicylate. The products were identified by nuclear magnetic resonance spectroscopy and three types of conversion were found. Streptomyces cinnamonensis and Streptomyces spectabilis formed gentisate and salicylamide concurrently. Streptomyces rimosus tra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-111
更新日期:1996-08-01 00:00:00
abstract::The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-141
更新日期:1978-07-01 00:00:00
abstract::Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains. The alternation of efficienc...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-177
更新日期:1985-10-01 00:00:00
abstract::The attachment of Bradyrhizobium sp. (Lupinus) strain MSDJ718 to excised roots of white lupin was examined. Maximal attachment occurred at early to middle log phases of bacterial growth. This binding was pH dependent, with an optimal value reached at 6.6. Irrespective of the culture age, the attachment was strongly af...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-035
更新日期:1996-03-01 00:00:00
abstract::Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w98-225
更新日期:1999-02-01 00:00:00
abstract::A tryptophan auxotroph of aerobically grown Rodospirillum rubrum was isolated after mutagenesis and replica plating. The mutant grows on tryptophan or indole, accumulates indole glycerol phosphate, lacks the capacity to convert indole glycerol phosphate to indole glycerol phosphate to indole, and finally is defective ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-055
更新日期:1978-03-01 00:00:00
abstract::Using a gene fusion approach, hybrid proteins were created by linking alkaline phosphatase (PhoA) or a cellulase reporter (delta CenA) to four large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein (RsaA; 1026 amino acids). Three of the sites (amino acids 189, 220, 315) were selected on the ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-122
更新日期:1994-09-01 00:00:00
abstract::Colletotrichum gloeosporioides is the causal agent of rubber tree anthracnose and leads to serious losses of natural rubber production. The pathogenesis of C. gloeosporioides on rubber tree remains unknown. Cerato-platanin proteins are small, secreted cysteine-rich proteins that contribute to virulence and function in...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0087
更新日期:2018-11-01 00:00:00
abstract::Legionella pneumophila grows in amoebae and has achieved the ability to grow at various temperatures, although the mechanisms controlling this ability remain poorly understood. The Icm/Dot type IVB secretion system is composed of more than 25 proteins and is known to be essential for intracellular growth. The role of...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-011
更新日期:2012-04-01 00:00:00
abstract::Highly distinctive aspects of the exponentail-phase Rhizobium japonicum cell were disclosed by means of thin sections, freeze etching, fluorescent antibodies, and ruthenium red staining. Polarity was expressed in the form of reserve polymer distribution near one end of the cell and as cytoplasmic localization near the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-191
更新日期:1977-09-01 00:00:00
abstract::The deliberate application of the methodology of contemporary molecular genetics to problems in bacterial systematics has led to a broad new understanding of the evolutionary history of both prokaryotes and eukaryotes. In this review, I discuss some of the major conclusions of this endeavour and try to predict future ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m88-093
更新日期:1988-04-01 00:00:00
abstract::A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-155
更新日期:1977-08-01 00:00:00
abstract::Three types of bacteria are associated with leprosy: Mycobacterium leprae, leprosy-derived corynebacteria (LDC), and armadillo-derived mycobacteria (ADM). The immunological relationships between these three types of bacteria and Mycobacterium bovis BCG, used as a reference, were determined by cross-immunoelectrophores...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-083
更新日期:1994-06-01 00:00:00
abstract::Screening for mycotoxin production by Chaetomium spp. and related fungi on rice culture was conducted by a combination of cytotoxicity tests using HeLa cells and thin-layer chromatography. Producers of sterigmatocystin, O-methylsterigmatocystin, chaetochromin, chaetocin, chetomin, cochliodinols, and mollicellin G were...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-119
更新日期:1981-08-01 00:00:00