A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil.

abstract：:The peptidoglycan of Escherichia coli K-12 strain AB264 was isolated by treating whole cells with sodium dodecyl sulfate and was purified by deoxyribonuclease, ribonuclease, and trypsin treatment. Like the peptidoglycan of Bacillus subtilis, this peptidoglycan proved able to bind substantial amounts of metallic ions f...

journal_title：Canadian journal of microbiology

authors： Hoyle BD,Beveridge TJ

更新日期：1984-02-01 00:00:00

abstract：:The expression of the toxA and regA genes in Pseudomonas aeruginosa is negatively regulated by iron at the transcriptional level. We have previously described ptxR, an exotoxin A regulatory gene which appears to enhance toxA expression through regA. In this study, we have tried to determine if ptxR expression correlat...

journal_title：Canadian journal of microbiology

authors： Colmer JA,Hamood AN

更新日期：1999-12-01 00:00:00

abstract：:Isolated toluene-degrading Pseudomonas stutzeri ST-9 bacteria were grown in a minimal medium containing toluene (100 mg·L(-1)) (MMT) or glucose (MMG) as the sole carbon source, with specific growth rates of 0.019 h(-1) and 0.042 h(-1), respectively. Scanning (SEM) as well as transmission (TEM) electron microscope anal...

journal_title：Canadian journal of microbiology

authors： Michael E,Nitzan Y,Langzam Y,Luboshits G,Cahan R

更新日期：2016-08-01 00:00:00

abstract：:A dairy product processing plant was studied for 2.5 years to examine contamination with Staphylococcus aureus and try to correlate the source of contamination. Cultures were submitted to an antibiotic susceptibility test (AST) and characterised by Pulsed-field Gel Electrophoresis (PFGE) analysis. Results showed that ...

journal_title：Canadian journal of microbiology

authors： Tondo EC,Guimarães MC,Henriques JA,Ayub MA

更新日期：2000-12-01 00:00:00

abstract：:The susceptibility of congenitally immunodeficient mice to a nonencapsulated strain of Cryptococcus neoformans (strain M7) was evaluated. Gnotobiotic mice with defined congenital defects in innate immunity (beige) or cell-mediated immunity (athymic) or with combined defects in innate and cellular immunity (beige athym...

journal_title：Canadian journal of microbiology

authors： Salkowski CA,Balish E

更新日期：1991-11-01 00:00:00

abstract：:Several strains of Rhizobium japonicum have been reported to consist of mixtures of stable derivativess having distinct colony morphologies and physiological characteristics. We isolated derivatives from strains of R. japonicum and systematically compared them with previously isolated derivatives with respect to the u...

journal_title：Canadian journal of microbiology

authors： Meyer MC,Pueppke SG

更新日期：1980-05-01 00:00:00

abstract：:Examination of immune complexes between cells of Streptococcus mutans and homologous antiserum by the techniques of thin-sectioning and freeze-etching revealed that the cells were embedded within and extensive matrix 80-90 nm thick with defined boundaries. ...

journal_title：Canadian journal of microbiology

authors： Emyanitoff RG,Rucinsky TE,Birdsell DC

更新日期：1976-06-01 00:00:00

abstract：:The aim of the present study was to examine soil samples from various vegetation zones in terms of physicochemical properties, microbial communities, and isolation and identification (by polymerase chain reaction and transmission electron microscopy) of bacteria producing poly-β-hydroxybutyrates (PHBs). Soil samples w...

journal_title：Canadian journal of microbiology

authors： Carpa R,Butiuc-Keul A,Lupan I,Barbu-Tudoran L,Muntean V,Dobrotă C

更新日期：2012-05-01 00:00:00

abstract：:Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...

journal_title：Canadian journal of microbiology

authors： Payment P,Ajdukovic D,Pavilanis V

更新日期：1975-05-01 00:00:00

abstract：:The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the st...

journal_title：Canadian journal of microbiology

authors： Laguerre M,Turcotte R

更新日期：1975-12-01 00:00:00

abstract：:We modified a technique, cDNA-AFLP, for identifying differentially expressed genes in plants to work in the filamentous fungus Leptosphaeria maculans (Desmaz.) Ces. & De Not. The cDNA fragments generated by our method ranged in size from approximately 100 to 400 bps. On average, twice as many cDNA fragments were ampli...

journal_title：Canadian journal of microbiology

authors： Gellatly KS,Ash GJ,Taylor JL

更新日期：2001-10-01 00:00:00

abstract：:Two potassium (K)-bearing minerals, Nanjing feldspar and Suzhou illite, were used to investigate K mobilization by the wild-type strain NBT of Bacillus edaphicus, also labeled MPs+, selected for high activity in mobilizing potassium from minerals, and by four of its UV + LiCl mutants, MPs++, MPs+1, MPs+2, and MPs-. In...

journal_title：Canadian journal of microbiology

authors： Sheng XF,He LY

更新日期：2006-01-01 00:00:00

abstract：:To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...

journal_title：Canadian journal of microbiology

authors： Dai J,Chen D,Gao G,Tang X,Wu S,Wu X,Zhou J

更新日期：2014-03-01 00:00:00

abstract：:For the recently described serotype 15 of biotype I and serotypes 13 and 14 of biotype II of Actinobacillus pleuropneumoniae, fhuA and hgbA were detected by polymerase chain reaction and DNA sequencing. To determine the substrate specificity of the iron receptors FhuA and HgbA and to study their role in the virulence ...

journal_title：Canadian journal of microbiology

authors： Shakarji L,Mikael LG,Srikumar R,Kobisch M,Coulton JW,Jacques M

更新日期：2006-04-01 00:00:00

abstract：:Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...

journal_title：Canadian journal of microbiology

authors： Koch Y,Rademacher KH

更新日期：1980-08-01 00:00:00

abstract：:The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...

journal_title：Canadian journal of microbiology

authors： Blakley ER

更新日期：1978-07-01 00:00:00

abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...

journal_title：Canadian journal of microbiology

authors： Pereira EM,Silva-Queiroz SR,Cabrera Gomez JG,Silva LF

更新日期：2009-06-01 00:00:00

abstract：:Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...

journal_title：Canadian journal of microbiology

authors： Gupta T,Fine-Coulson K,Karls R,Gauthier D,Quinn F

更新日期：2013-08-01 00:00:00

abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...

journal_title：Canadian journal of microbiology

authors： White AP,Allen-Vercoe E,Jones BW,DeVinney R,Kay WW,Surette MG

更新日期：2007-01-01 00:00:00

abstract：:Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...

journal_title：Canadian journal of microbiology

authors： Nyhlén L,Unestam T

更新日期：1978-11-01 00:00:00

abstract：:The effects of seven thiophosphorus pesticides and their degradation products on estuarine ammonium oxidizers were examined. Using an axenic, closed-culture, high cell density assay, the pesticides caused little inhibition of ammonium oxidation. However, the degradation product of methyl parathion, p-aminophenol, sign...

journal_title：Canadian journal of microbiology

authors： Jones RD,Hood MA

更新日期：1980-11-01 00:00:00

abstract：:Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...

journal_title：Canadian journal of microbiology

authors： Tejesvi MV,Kini KR,Prakash HS,Subbiah V,Shetty HS

更新日期：2008-09-01 00:00:00

abstract：:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...

journal_title：Canadian journal of microbiology

authors： Ogg JE,Lee SY,Ogg BJ

更新日期：1979-09-01 00:00:00

abstract：:Seven tk- mutants of herpes simplex virus, type 2 (HSV-2), and three tk- mutants of herpes simplex virus, type 1 (HSV-1), were isolated which did not produce the thymidine kinase (TK) polypeptides but formed smaller polypeptides not seen in wild-type infected cells. Positive TK mRNA selection by hybridization to the c...

journal_title：Canadian journal of microbiology

authors： Block TM,Kuhn NJ,Kustas KA,Held WA,Gross K,Hughes RG Jr

更新日期：1983-04-01 00:00:00

abstract：:Bacterial culture based on the use of plate media is an effective method for investigating bacterial populations in the environment. To improve the culturability of bacteria from freshwater lakes, we developed a new medium, FW70, which contains sodium pyruvate, casamino acids, and lake water and is solidified using ge...

journal_title：Canadian journal of microbiology

authors： Imazaki I,Kobori Y

更新日期：2010-04-01 00:00:00

abstract：:Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...

journal_title：Canadian journal of microbiology

authors： Fonty G,Gouet P,Nebout JM

更新日期：1989-03-01 00:00:00

abstract：:Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...

journal_title：Canadian journal of microbiology

authors： Shinagawa K,Omoe K,Matsusaka N,Sugii S

更新日期：1991-08-01 00:00:00

abstract：:In our study of opportunistic pathogens, we have some indication that Staphylococcus aureus can increase resistance in mice against Pseudomonas aeruginosa. Intraperitoneal injections of sublethal doses of S. aureus had a protective effect in mice against lethal doses of P. aeruginosa, more so if living and coagulase-p...

journal_title：Canadian journal of microbiology

authors： deRepentigny J,Mathieu LG,Coallier-Ascah J

更新日期：1976-07-01 00:00:00

abstract：:A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...

journal_title：Canadian journal of microbiology

authors： Blaghen M,Vidon DJ,el Kebbaj MS

更新日期：1993-02-01 00:00:00

abstract：:The presence and taxonomy of endophytic bacteria of the entire aerial parts of crocus (Crocus albiflorus), a wildflower native in the Alps, were investigated. A combination of plating of plant macerates, isolation and sequence identification of isolates, and direct 16S rDNA PCR amplification followed by whole-communit...

journal_title：Canadian journal of microbiology

authors： Reiter B,Sessitsch A

更新日期：2006-02-01 00:00:00