An efficient system for markerless gene replacement applicable in a wide variety of enterobacterial species.

abstract：:A quantitative and qualitative survey of the indigenous Endogone population in wheat field soil and vesicular-arbuscular mycorrhiza in wheat roots as influenced by season and by the stage of development of the wheat plant was made. The number of Endogone spores in wheat field soil remained relatively unchanged in win...

journal_title：Canadian journal of microbiology

authors： Saif SR,Khan AG

更新日期：1975-07-01 00:00:00

abstract：:Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...

journal_title：Canadian journal of microbiology

authors： Fons F,Amellal N,Leyval C,Saint-Martin N,Henry M

更新日期：2003-06-01 00:00:00

abstract：:The presence and taxonomy of endophytic bacteria of the entire aerial parts of crocus (Crocus albiflorus), a wildflower native in the Alps, were investigated. A combination of plating of plant macerates, isolation and sequence identification of isolates, and direct 16S rDNA PCR amplification followed by whole-communit...

journal_title：Canadian journal of microbiology

authors： Reiter B,Sessitsch A

更新日期：2006-02-01 00:00:00

abstract：:This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...

journal_title：Canadian journal of microbiology

authors： D'Elia TV,Cooper CR,Johnston CG

更新日期：2007-10-01 00:00:00

abstract：:The aim of this study was to determine the effect of the rhizosphere of maize on the diversity of denitrifying bacteria. Community structure comparison was performed by constructing a collection of isolates recovered from bulk and maize planted soil. A total of 3240 nitrate-reducing isolates were obtained and 188 of t...

journal_title：Canadian journal of microbiology

authors： Chèneby D,Perrez S,Devroe C,Hallet S,Couton Y,Bizouard F,Iuretig G,Germon JC,Philippot L

更新日期：2004-07-01 00:00:00

abstract：:Microbial mats that develop in shallow brackish and hyposaline ponds in the rims of two French polynesian atolls (Rangiroa and Tetiaroa) were intensively investigated during the past three years. Comparative assessment of these mats (called kopara in polynesian language) showed remarkable similarities in their composi...

journal_title：Canadian journal of microbiology

authors： Che LM,Andréfouët S,Bothorel V,Guezennec M,Rougeaux H,Guezennec J,Deslandes E,Trichet J,Matheron R,Le Campion T,Payri C,Caumette P

更新日期：2001-11-01 00:00:00

abstract：:The peptidoglycan of Escherichia coli K-12 strain AB264 was isolated by treating whole cells with sodium dodecyl sulfate and was purified by deoxyribonuclease, ribonuclease, and trypsin treatment. Like the peptidoglycan of Bacillus subtilis, this peptidoglycan proved able to bind substantial amounts of metallic ions f...

journal_title：Canadian journal of microbiology

authors： Hoyle BD,Beveridge TJ

更新日期：1984-02-01 00:00:00

abstract：:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...

journal_title：Canadian journal of microbiology

authors： Figueira MM,Ciminelli VS,de Andrade MC,Linardi VR

更新日期：1996-05-01 00:00:00

abstract：:Three types of bacteria are associated with leprosy: Mycobacterium leprae, leprosy-derived corynebacteria (LDC), and armadillo-derived mycobacteria (ADM). The immunological relationships between these three types of bacteria and Mycobacterium bovis BCG, used as a reference, were determined by cross-immunoelectrophores...

journal_title：Canadian journal of microbiology

authors： Baelden MC,Bouckaert AE,Grégoire D,van de Poele M,Coene M

更新日期：1994-06-01 00:00:00

abstract：:Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...

journal_title：Canadian journal of microbiology

authors： Buchrieser C,Brosch R,Catimel B,Rocourt J

更新日期：1993-04-01 00:00:00

abstract：:The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...

journal_title：Canadian journal of microbiology

authors： Mehta RJ,Nash CH

更新日期：1979-07-01 00:00:00

abstract：:The immune benefits associated with the optimal intake of dietary fatty acids are widely known. The objective of the present investigation was to elucidate the role of omega-3 polyunsaturated fatty acids (n-3 PUFA) food source on acute pneumonia induced by Klebsiella pneumoniae. Three different n-3 PUFA preparations (...

journal_title：Canadian journal of microbiology

authors： Sharma S,Chhibber S,Mohan H,Sharma S

更新日期：2013-07-01 00:00:00

abstract：:The antibacterial mechanism of a Cinnamomum cassia essential oil from Vietnam and of its main component (trans-cinnamaldehyde, 90% (m/m) of C. cassia essential oil) against a Listeria innocua strain was investigated to estimate their potential for food preservation. In the presence of C. cassia essential oil or trans-...

journal_title：Canadian journal of microbiology

authors： Trinh NT,Dumas E,Thanh ML,Degraeve P,Ben Amara C,Gharsallaoui A,Oulahal N

更新日期：2015-04-01 00:00:00

abstract：:The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investig...

journal_title：Canadian journal of microbiology

authors： Shen D,Ye W,Dong S,Wang Y,Dou D

更新日期：2011-02-01 00:00:00

abstract：:Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...

journal_title：Canadian journal of microbiology

authors： Syed MA,Lévesque CM

更新日期：2012-05-01 00:00:00

abstract：:Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...

journal_title：Canadian journal of microbiology

authors： Richardson AE,Hadobas PA

更新日期：1997-06-01 00:00:00

abstract：:The diversity and variation of total and active ammonia-oxidizing bacteria in a full-scale aerated submerged biofilm reactor for drinking water pretreatment were characterized by clone libraries and denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA and its gene during a whole year. Sequences obtained...

journal_title：Canadian journal of microbiology

authors： Zhang XW,Qin YY,Ren HQ,Li DT,Yang H

更新日期：2008-05-01 00:00:00

abstract：:Through gas chromatography - mass spectrometry, the presence of oxylipins, mainly 3-hydroxy 9:1 and 3-hydroxy 10:1, was detected in Saccharomycopsis fermentans, Saccharomycopsis javanensis, and Saccharomycopsis vini. The distribution of these compounds was mapped using immunofluorescence microscopy, and they were foun...

journal_title：Canadian journal of microbiology

authors： Sebolai OM,Kock JL,Pohl CH,Botes PJ,Strauss CJ,Van Wyk PW,Nigam S

更新日期：2005-07-01 00:00:00

abstract：:Urinary tract infections are common. Few published studies have demonstrated the change in Escherichia coli urinary isolate antimicrobial susceptibility over time within a given area and (or) population. The purpose of this study was to evaluate the change in susceptibility of E. coli clinical isolates obtained from u...

journal_title：Canadian journal of microbiology

authors： Walkty A,Lagacé-Wiens PR,Karlowsky JA,Hoban DJ,Manickam K,Adam H,Pieroni P,Alfa M

更新日期：2012-03-01 00:00:00

abstract：:We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...

journal_title：Canadian journal of microbiology

authors： Bohnen JM,Matlow AG,Mustard RA,Christie NA,Kavouris B

更新日期：1988-03-01 00:00:00

abstract：:The use of microorganisms for bioremediation of contaminated soils may be enhanced with an understanding of the pathways involved in their degradation of hazardous compounds. Ralstonia sp. strain RJGII.123 was isolated from soil located at a former coal gasification plant, based on its ability to mineralize carbazole,...

journal_title：Canadian journal of microbiology

authors： Schneider J,Grosser RJ,Jayasimhulu K,Xue W,Kinkle B,Warshawsky D

更新日期：2000-03-01 00:00:00

abstract：:Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-deriv...

journal_title：Canadian journal of microbiology

authors： Daniels JB,Scoffield J,Woolnough JL,Silo-Suh L

更新日期：2014-12-01 00:00:00

abstract：:Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...

journal_title：Canadian journal of microbiology

authors： Johnson KG,McDonald IJ

更新日期：1976-03-01 00:00:00

abstract：:Phytopathogenic spiroplasmas can multiply in vascular plants and insects. A deeper understanding of this dual-host life could be furthered through the identification by random mutagenesis of spiroplasma genes required. The ability of the EZ::TN™ Tnp transposome™ system to create random insertional mutations i...

journal_title：Canadian journal of microbiology

authors： Mutaqin K,Comer JL,Wayadande AC,Melcher U,Fletcher J

更新日期：2011-06-01 00:00:00

abstract：:During shadowing, a "cap" of metal develops on small particles. This cap increases apparent particle with (measured normal to the shadowing direction) by an extent which cannot be predetermined. The extent of this increase in particle size (here defined as the "cap," X) is estimated in the present method by using oppo...

journal_title：Canadian journal of microbiology

authors： Willison JH

更新日期：1985-07-01 00:00:00

abstract：:An obligate methyltroph Methylomonas methylovora oxidized methylamine, formaldehyde, and formate. Enzymes oxidizing these substrates were detected in a cell-free system. Phenazine methosulfate-linked methylamine dehydrogenase was purified 21-fold. The enzyme had optimum activity at pH 7.5 and was stable at 60 degrees ...

journal_title：Canadian journal of microbiology

authors： Mehta RJ

更新日期：1977-04-01 00:00:00

abstract：:Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. ...

journal_title：Canadian journal of microbiology

authors： Konowalchuk J,Spiers JI

更新日期：1977-09-01 00:00:00

abstract：:To determine the subcellular location of the Streptococcus mutans P1 protein C-terminal anchor, cell envelope fractionation experiments were conducted in combination with Western immunoblotting, using monoclonal antibody MAb 6-8C specific for an epitope that maps near the C terminus of P1 protein and also a polyclonal...

journal_title：Canadian journal of microbiology

authors： Homonylo-McGavin MK,Lee SF,Bowden GH

更新日期：1999-06-01 00:00:00

abstract：:Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...

journal_title：Canadian journal of microbiology

authors： Payment P,Ajdukovic D,Pavilanis V

更新日期：1975-05-01 00:00:00

abstract：:Formate dehydrogenase activity (EC 1.2.1.2) has been demonstrated in cell-free preparations of Mycobacterium phlei by following the reduction of 2,6 dichlorophenolindophenol. thiazolyl blue tetrazolium, or equine cytochrome c. The reduction of equine cytochrome c was inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide. N...

journal_title：Canadian journal of microbiology

authors： Deyhle RR,Barton LL

更新日期：1977-02-01 00:00:00