Characterization of intronic structures and alternative splicing in Phytophthora sojae by comparative analysis of expressed sequence tags and genomic sequences.


:The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investigated the introns of Phytophthora sojae, a pathogen that causes soybean root and stem rot, by a comparative analysis of genomic sequences and expressed sequence tags. A total of 4013 introns were identified, of which 96.6% contained canonical splice sites. The P. sojae genome possessed features distinct from other organisms at 5' splice sites, polypyrimidine tracts, branch sites, and 3' splice sites. Diverse repeating sequences, ranging from 2 to 10 nucleotides in length, were found at more than half of the intron-exon boundaries. Furthermore, 122 genes underwent alternative splicing. These data indicate that P. sojae has unique splicing mechanisms, and recognition of those mechanisms may lead to more accurate predictions of the location of introns in P. sojae and even other oomycete species.


Can J Microbiol


Shen D,Ye W,Dong S,Wang Y,Dou D




Has Abstract


2011-02-01 00:00:00














  • Use of mutants to establish (+)-scytalone as an intermediate in melanin biosynthesis by Verticillium dahliae.

    abstract::Melanin biosynthesis in Verticillium dahliae Kleb, was studied with mutants deficient for normal black melanin or for production of microsclerotia. Seven genetically different mutants had apparent blocks in melanin biosynthesis. Four mutants (brm-1 to -4) produced brown microsclerotia and extruded pigments into media;...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Bell AA,Puhalla JE,Tolmsoff WJ,Stipanovic RD

    更新日期:1976-06-01 00:00:00

  • Persistence of naturally acquired cell-mediated immunity to rubella virus during the first trimester of pregnancy.

    abstract::We studied the persistence of naturally acquired cell-mediated immunity to rubella during early pregnancy. We compared lymphocyte transformation responses to phytohemagglutinin and rubella virus in 35 naturally immune women aged 17-37 years, in the first trimester of pregnancy, with 49 naturally immune age-matched non...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Rossier E,Phipps PH,Weber JM

    更新日期:1983-12-01 00:00:00

  • The interaction of cholera toxin subunit A with cultured adrenal cells.

    abstract::Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Knoop FC

    更新日期:1978-08-01 00:00:00

  • A modified tube method for the cultivation and enumeration of anaerobic bacteria.

    abstract::A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Ogg JE,Lee SY,Ogg BJ

    更新日期:1979-09-01 00:00:00

  • Selection and characterization of Spiroplasma citri mutants by random transposome mutagenesis.

    abstract::Phytopathogenic spiroplasmas can multiply in vascular plants and insects. A deeper understanding of this dual-host life could be furthered through the identification by random mutagenesis of spiroplasma genes required. The ability of the EZ::TN™ Tnp transposome™ system to create random insertional mutations i...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Mutaqin K,Comer JL,Wayadande AC,Melcher U,Fletcher J

    更新日期:2011-06-01 00:00:00

  • Soil microbial communities of three grassland ecosystems in the Bayinbuluke, China.

    abstract::The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Shao K,Gao G

    更新日期:2018-03-01 00:00:00

  • Ethanol and carbon-source starvation enhance the accumulation of HSP80 in Neurospora crassa.

    abstract::In Neurospora crassa, heat shock results in the induction of 9 to 11 heat shock proteins (HSP), of which HSP80 is the most abundant and the first to be synthesized. The induction of HSP80 was investigated during normal growth (2% sucrose) and under sucrose starvation. Transfer of mycelium to a medium supplemented with...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Roychowdhury HS,Kapoor M

    更新日期:1988-02-01 00:00:00

  • Effect of some environmental parameters on fermentative hydrogen production by Enterobacter cloacae DM11.

    abstract::Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.3...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Nath K,Kumar A,Das D

    更新日期:2006-06-01 00:00:00

  • Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor.

    abstract::Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Al-Aidroos K,Bussey H

    更新日期:1978-03-01 00:00:00

  • Microbial communities in the larval midgut of laboratory and field populations of cotton bollworm (Helicoverpa armigera).

    abstract::We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Xiang H,Wei GF,Jia S,Huang J,Miao XX,Zhou Z,Zhao LP,Huang YP

    更新日期:2006-11-01 00:00:00

  • Trichosporon guehoae sp.nov., an anamorphic basidiomycetous yeast.

    abstract::A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Middelhoven WJ,Scorzetti G,Fell JW

    更新日期:1999-08-01 00:00:00

  • Modulation of carbohydrate-binding capacities and attachment ability of Bradyrhizobium sp. (lupinus) to white lupin roots.

    abstract::The attachment of Bradyrhizobium sp. (Lupinus) strain MSDJ718 to excised roots of white lupin was examined. Maximal attachment occurred at early to middle log phases of bacterial growth. This binding was pH dependent, with an optimal value reached at 6.6. Irrespective of the culture age, the attachment was strongly af...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Wisniewski JP,Delmotte FM

    更新日期:1996-03-01 00:00:00

  • Characterization of a clinical Vibrio cholerae O139 isolate from Mexico.

    abstract::Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Parveen S,Farrah SR,Gonzalez-Bonilla C,Zamudio AV,Tamplin ML

    更新日期:2003-01-01 00:00:00

  • Exposure of Agrobacterium tumefaciens to agroinfiltration medium demonstrates cellular remodelling and may promote enhanced adaptability for molecular pharming.

    abstract::Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Prudhomme N,Pastora R,Muselius B,McLean MD,Cossar D,Geddes-McAlister J

    更新日期:2021-01-01 00:00:00

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    abstract::The diversity and variation of total and active ammonia-oxidizing bacteria in a full-scale aerated submerged biofilm reactor for drinking water pretreatment were characterized by clone libraries and denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA and its gene during a whole year. Sequences obtained...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Zhang XW,Qin YY,Ren HQ,Li DT,Yang H

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  • Study of characteristics on metabolism of Penicillium chrysogenum F1 during bioleaching of heavy metals from contaminated soil.

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    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Deng X,Yang Z,Chen R

    更新日期:2019-09-01 00:00:00

  • Comparison of element levels in minimal and complex yeast media.

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    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Abelovska L,Bujdos M,Kubova J,Petrezselyova S,Nosek J,Tomaska L

    更新日期:2007-04-01 00:00:00

  • Interaction of lectins with Neisseria gonorrhoeae.

    abstract::Fifty-four strains of Neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. Wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. Dolichos biflorus, Sophora japonica, Maclura pomifera, Ulex Europaeus, Lens culinaris, Canavalia ensifo...

    journal_title:Canadian journal of microbiology

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    authors: Allen PZ,Connelly MC,Apicella MA

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  • Immune responses of mice to inactivated rabies vaccine administered orally: potentiation by Quillaja saponin.

    abstract::Administered orally, Quillaja saponin markedly potentiated the humoral immune responses of mice fed inactivated rabies vaccine, and significantly increased their resistance to subsequent intracerebral challenge with live rabies virus. Although mean serum neutralizing antibody titres were generally 8- to 16-fold higher...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Maharaj I,Froh KJ,Campbell JB

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  • The origin of fatty acids in the hydrocarbon-utilizing microorganism Mycobacterium vaccae.

    abstract::The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: King DH,Perry JJ

    更新日期:1975-01-01 00:00:00

  • Use of protein microarray to identify gene expression changes of Yersinia pestis at different temperatures.

    abstract::Yersinia pestis is a bacterium that is transmitted between fleas, which have a body temperature of 26 °C, and mammalian hosts, which have a body temperature of 37 °C. To adapt to the temperature shift, phenotype variations, including virulence, occur. In this study, an antigen microarray including 218 proteins of Y. p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Li B,Tan Y,Guo J,Cui B,Wang Z,Wang H,Zhou L,Guo Z,Zhu Z,Du Z,Yang R

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  • Test for differentiation of M. tuberculosis and M. bovis from other mycobacteria.

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    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Laszlo A,Eidus L

    更新日期:1978-06-01 00:00:00

  • Overproduction of the Bacillus subtilis glutamyl-tRNA synthetase in its host and its toxicity to Escherichia coli.

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    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Pelchat M,Lacoste L,Yang F,Lapointe J

    更新日期:1998-04-01 00:00:00

  • The effect of decomplementation on the infectious course of Sendai virus in mice.

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    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Finnie JC,Aston WP,O'Shaughnessy MV,Stewart RB

    更新日期:1982-05-01 00:00:00

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    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Abranches J,Morais PB,Rosa CA,Mendonça-Hagler LC,Hagler AN

    更新日期:1997-04-01 00:00:00

  • Chemical and serological properties of a cyanogen bromide peptide of southern bean mosaic virus protein.

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    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Tremaine JH,Ronald WP,Kelly EM

    更新日期:1980-12-01 00:00:00

  • Hydrogen cyanide production by Pseudomonas aeruginosa at reduced oxygen levels.

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    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Castric PA

    更新日期:1983-10-01 00:00:00

  • Relationship between clinical site of isolation and ability to form biofilms in vitro in nontypeable Haemophilus influenzae.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Obaid NA,Jacobson GA,Tristram S

    更新日期:2015-03-01 00:00:00

  • Nitrogen fixation in the muskeg ecosystem of the James Bay Lowlands, Northern Ontario.

    abstract::The acetylene-reduction assay was used for in situ and laboratory assessment of biological nitrogen fixation in the acidic, waterlogged, muskeg ecosystem of the southern James Bay area, in the region of Moosonee, Ontario. In situ assays and subsequent laboratory experimentation revealed that nitrogenase activity was p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Blasco JA,Jordan DC

    更新日期:1976-07-01 00:00:00

  • Genotyping of Yersinia enterocolitica biotype 1A strains from clinical and nonclinical origins by pulsed-field gel electrophoresis.

    abstract::Yersinia enterocolitica biotype 1A (B1A) strains are considered mainly nonpathogenic. However, some studies considered strains of this biotype to be the causal agents of infections in humans and animals. In South America, there are no studies that have compared clinical and nonclinical strains of B1A typed by pulsed-f...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Campioni F,Falcão JP

    更新日期:2014-06-01 00:00:00