Characterization of intronic structures and alternative splicing in Phytophthora sojae by comparative analysis of expressed sequence tags and genomic sequences.

abstract：:Purification and chemical characterization of an immunosuppressive exopolysaccharide from Capnocytophaga ochracea strain 25 are described. This polysaccharide was extracted from spent culture medium by cold ethanol precipitation. Purification was accomplished by trichloroacetic acid and pronase treatments in combinati...

journal_title：Canadian journal of microbiology

authors： Dyer JK,Bolton RW

更新日期：1985-01-01 00:00:00

abstract：:Ten bacterial strains were isolated by enrichment culture, using as carbon sources either aliphatics or an aromatic-polar mixture. Oxygen uptake rate was used as a criterion to determine culture transfer timing at each enrichment stage. Biodegradation of aliphatics (10,000 mg L(-1)) and an aromatic-polar mixture (5000...

journal_title：Canadian journal of microbiology

authors： Díaz-Ramírez IJ,Ramírez-Saad H,Gutiérrez-Rojas M,Favela-Torres E

更新日期：2003-12-01 00:00:00

abstract：:The N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain Marburg has been purified with reasonable yield and much higher specific activity than previously reported. For the first time it has been shown that both N5,N10-methylenetetrahydromethanopterin dehydrogenase and...

journal_title：Canadian journal of microbiology

authors： Mukhopadhyay B,Daniels L

更新日期：1989-04-01 00:00:00

abstract：:The ruminal microbiota plays major roles in feed digestion. The composition and fermentation of the bacterial communities in 3 important ruminant species have been studied previously. Here, we extended this research to the effect of concentrate-to-forage ratios on ruminal bacterial communities in Tarim red deer (Cervu...

journal_title：Canadian journal of microbiology

authors： Qian W,Ao W,Hui X,Wu J

更新日期：2018-07-01 00:00:00

abstract：:In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...

journal_title：Canadian journal of microbiology

authors： Chang KP,Musgrave AJ

更新日期：1975-02-01 00:00:00

abstract：:The aim of this study was to unravel, by focusing on cell surface properties, the underlying virulence factors contributing to the difference in the pathogenicity observed in two Acinetobacter baumannii strains isolated from the same patient. The two strains were phenotypically different: (i) a mucoid strain (AB-M),...

journal_title：Canadian journal of microbiology

authors： Kempf M,Eveillard M,Deshayes C,Ghamrawi S,Lefrançois C,Georgeault S,Bastiat G,Seifert H,Joly-Guillou ML

更新日期：2012-03-01 00:00:00

abstract：:Two molecular forms of alpha-galactosidase (EC 3.2.1.22) synthesized constitutively by Bacillus stearothermophilus, strain AT-7, have been purified. alpha-Galactosidase I (with the substrate p-nitrophenyl alpha-D-galactopyranoside (PNPG)) has a pH optimum of 6 and half-life at 65 degrees C of > 2 h at low protein conc...

journal_title：Canadian journal of microbiology

authors： Pederson DM,Goodman RE

更新日期：1980-08-01 00:00:00

abstract：:Verticillium fungicola, isolated from Agaricus bisporus (commercial mushroom), produced significant extracellular hydrophobin when grown for 7 days in a static liquid culture of synthetic minimal medium. The hydrophobin was purified by precipitation with ammonium sulphate (80% saturation), Sephadex G-100 gel filtratio...

journal_title：Canadian journal of microbiology

authors： Calonje M,Bernardo D,Novaes-Ledieu M,García Mendoza C

更新日期：2002-11-01 00:00:00

abstract：:The induction of nitrogenase (C2H2) activity in asymbiotically cultured Rhizobium sp. 32H1 was found to be associated with morphological changes in the cells which were more pronounced than those seen in bacteroids. Polyphosphate granules were found in both bacteroids and cultured cells, but poly-beta-hydroxybutyrate ...

journal_title：Canadian journal of microbiology

authors： van Brussel AA,Costerton JW,Child JJ

更新日期：1979-03-01 00:00:00

abstract：:Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...

journal_title：Canadian journal of microbiology

authors： Castric PA

更新日期：1983-10-01 00:00:00

abstract：:A Tn551 insertional pleiotropic mutant defective in the production of several exoproteins was isolated from Staphylococcus aureus 196E and characterized. The pleiotropism of the mutant was due to a single insertion of the transposon as evidenced by Southern blot hybridization and by the transfer of its phenotype by tr...

journal_title：Canadian journal of microbiology

authors： Giraudo AT,Raspanti CG,Calzolari A,Nagel R

更新日期：1994-08-01 00:00:00

abstract：:Boron (B) is toxic to living cells at levels above a certain threshold. We isolated several B-tolerant bacterial strains from soil samples and studied them for possible mechanisms of B tolerance. 16S rRNA gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 gen...

journal_title：Canadian journal of microbiology

authors： Ahmed I,Fujiwara T

更新日期：2010-01-01 00:00:00

abstract：:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...

journal_title：Canadian journal of microbiology

authors： Pelchat M,Lacoste L,Yang F,Lapointe J

更新日期：1998-04-01 00:00:00

abstract：:The oligosaccharides of human lactoferrin were enzymatically removed with glycopeptidase F, resulting in a preparation containing partial and fully deglycosylated human lactoferrin. The derivatives were separated by Concanavalin A affinity chromatography and compared with native human lactoferrin with respect to their...

journal_title：Canadian journal of microbiology

authors： Alcantara J,Padda JS,Schryvers AB

更新日期：1992-11-01 00:00:00

abstract：:Aspergillus fumigatus conidia have been linked to severe aspergillosis in immunocompromised patients. Recently, the cytotoxic effect of secondary metabolites from A. fumigatus conidia was reported. In the present work, a methodology used to detect cell death markers in fungal hyphae was adapted to study conidia cell d...

journal_title：Canadian journal of microbiology

authors： Oliveira M,Pereira C,Bessa C,Araujo R,Saraiva L

更新日期：2016-02-01 00:00:00

abstract：:To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...

journal_title：Canadian journal of microbiology

authors： Dai J,Chen D,Gao G,Tang X,Wu S,Wu X,Zhou J

更新日期：2014-03-01 00:00:00

abstract：:Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...

journal_title：Canadian journal of microbiology

authors： Tremaine JH,Ronald WP,Kelly EM

更新日期：1980-12-01 00:00:00

abstract：:Methanospirillum hungatei strains GP1 and JF1 when exposed to the adenosinetriphosphatase inhibitor N,N'-dicyclohexylcarbodiimide experienced a marked decline in growth rates, methane synthesis activities, and intracellular ATP concentrations. Although growth was inhibited, the intracellular ATP concentrations of all ...

journal_title：Canadian journal of microbiology

authors： Sprott GD,Jarrell KF

更新日期：1982-08-01 00:00:00

abstract：:Scanning and transmission electron microscopy was applied for a morphological study of three strains of Bifidobacterium grown on solid or liquid media. The pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. A possible sequence of the morphological events during transformatio...

journal_title：Canadian journal of microbiology

authors： Bauer H,Sigarlakie E,Faure JC

更新日期：1975-09-01 00:00:00

abstract：:Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high p...

journal_title：Canadian journal of microbiology

authors： Hammad Y,Maréchal J,Cournoyer B,Normand P,Domenach AM

更新日期：2001-06-01 00:00:00

abstract：:Mutations in the DNA polymerase locus of phage, bacteria, and eukaryotic may change the mutation rates at other loci of the genome. We used resistance to phosphonoacetate to select mutants of herpes simplex virus with mutated DNA polymerase and then determined the reversion frequency of viral thymidine kinase mutation...

journal_title：Canadian journal of microbiology

authors： Campione-Piccardo J,Rawls WE

更新日期：1984-05-01 00:00:00

abstract：:Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...

journal_title：Canadian journal of microbiology

authors： Fons F,Amellal N,Leyval C,Saint-Martin N,Henry M

更新日期：2003-06-01 00:00:00

abstract：:We studied the persistence of naturally acquired cell-mediated immunity to rubella during early pregnancy. We compared lymphocyte transformation responses to phytohemagglutinin and rubella virus in 35 naturally immune women aged 17-37 years, in the first trimester of pregnancy, with 49 naturally immune age-matched non...

journal_title：Canadian journal of microbiology

authors： Rossier E,Phipps PH,Weber JM

更新日期：1983-12-01 00:00:00

abstract：:Phytopathogenic spiroplasmas can multiply in vascular plants and insects. A deeper understanding of this dual-host life could be furthered through the identification by random mutagenesis of spiroplasma genes required. The ability of the EZ::TN™ Tnp transposome™ system to create random insertional mutations i...

journal_title：Canadian journal of microbiology

authors： Mutaqin K,Comer JL,Wayadande AC,Melcher U,Fletcher J

更新日期：2011-06-01 00:00:00

abstract：:The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size di...

journal_title：Canadian journal of microbiology

authors： Ishiguro EE,Ramey WD

更新日期：1978-06-01 00:00:00

abstract：:Pleomorphic deterioration is a process where a fungal isolate loses the ability to produce conidia during repeated subculturing. We have previously isolated strains of the entomopathogenic fungus Metarhizium anisopliae that have irreversibly lost the ability to produce conidia and only produce mycelia when grown on ag...

journal_title：Canadian journal of microbiology

authors： Kamp AM,Bidochka MJ

更新日期：2002-09-01 00:00:00

abstract：:The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...

journal_title：Canadian journal of microbiology

authors： Mehta RJ,Nash CH

更新日期：1979-07-01 00:00:00

abstract：:Effects of Trichoderma harzianum and T. polysporum on chlamydospore production by two isolates of Phytophthora cinamomi were determined over a 21-day period in nonsterile, sterilized, and sterilized amended soil. Trichoderma was either coinoculated with P. cinnamomi or added to the cultures on day 3 of the incubation ...

journal_title：Canadian journal of microbiology

authors： Kelley WD

更新日期：1977-03-01 00:00:00

abstract：:This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...

journal_title：Canadian journal of microbiology

authors： Wicklow DT,Shotwell OL

更新日期：1983-01-01 00:00:00

abstract：:Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...

journal_title：Canadian journal of microbiology

authors： Samuelson DA,Bezerra J

更新日期：1977-10-01 00:00:00