Characterization of intronic structures and alternative splicing in Phytophthora sojae by comparative analysis of expressed sequence tags and genomic sequences.


:The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investigated the introns of Phytophthora sojae, a pathogen that causes soybean root and stem rot, by a comparative analysis of genomic sequences and expressed sequence tags. A total of 4013 introns were identified, of which 96.6% contained canonical splice sites. The P. sojae genome possessed features distinct from other organisms at 5' splice sites, polypyrimidine tracts, branch sites, and 3' splice sites. Diverse repeating sequences, ranging from 2 to 10 nucleotides in length, were found at more than half of the intron-exon boundaries. Furthermore, 122 genes underwent alternative splicing. These data indicate that P. sojae has unique splicing mechanisms, and recognition of those mechanisms may lead to more accurate predictions of the location of introns in P. sojae and even other oomycete species.


Can J Microbiol


Shen D,Ye W,Dong S,Wang Y,Dou D




Has Abstract


2011-02-01 00:00:00














  • Ultrastructural effects of lysozymes on the cell wall of Caryophanon latum.

    abstract::When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the s...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Trentini WC,Murray RG

    更新日期:1975-02-01 00:00:00

  • Scanning and transmission electron microscopy of three strains of Bifidobacterium.

    abstract::Scanning and transmission electron microscopy was applied for a morphological study of three strains of Bifidobacterium grown on solid or liquid media. The pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. A possible sequence of the morphological events during transformatio...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Bauer H,Sigarlakie E,Faure JC

    更新日期:1975-09-01 00:00:00

  • Microbial communities in the larval midgut of laboratory and field populations of cotton bollworm (Helicoverpa armigera).

    abstract::We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Xiang H,Wei GF,Jia S,Huang J,Miao XX,Zhou Z,Zhao LP,Huang YP

    更新日期:2006-11-01 00:00:00

  • Regulation and properties of an invertase from Clostridium pasteurianum.

    abstract::An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Laishley EJ

    更新日期:1975-11-01 00:00:00

  • The effect of farnesol on amino acid incorporation by a wild-type and cell-wall variant strain of Candida albicans.

    abstract::Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 microg/mL in yeast nitrogen bro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Braun PC

    更新日期:2005-08-01 00:00:00

  • Leakage of periplasmic enzymes from lipopolysaccharide-defective mutants of Salmonella typhimurium.

    abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC, cyclic phosphodiesterase, ribonuclease I (EC, and phosphoglucose isomerase (EC (PGI is at least par...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Chatterjee AK,Ross H,Sanderson KE

    更新日期:1976-10-01 00:00:00

  • Effect of toluene on Pseudomonas stutzeri ST-9 morphology - plasmolysis, cell size, and formation of outer membrane vesicles.

    abstract::Isolated toluene-degrading Pseudomonas stutzeri ST-9 bacteria were grown in a minimal medium containing toluene (100 mg·L(-1)) (MMT) or glucose (MMG) as the sole carbon source, with specific growth rates of 0.019 h(-1) and 0.042 h(-1), respectively. Scanning (SEM) as well as transmission (TEM) electron microscope anal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Michael E,Nitzan Y,Langzam Y,Luboshits G,Cahan R

    更新日期:2016-08-01 00:00:00

  • The fine structure of conidial development in the genus Torula. II. T. caligans (Batista and Upadhyay) M. B. Ellis and T. terrestris Mistra.

    abstract::Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Ellis DH,Griffiths DA

    更新日期:1975-12-01 00:00:00

  • Determination of peptidyl dipeptidase activity in 24 bacterial species.

    abstract::Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity th...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Stevens J,Fanburg BL,Lanzillo JJ

    更新日期:1990-01-01 00:00:00

  • Cloning of a xylanase gene from the ruminal fungus Neocallimastix patriciarum 27 and its expression in Escherichia coli.

    abstract::An endo-beta-1,4-xylanase gene was cloned from Neocallimastix patriciarum 27 in the bacteriophage vector lambda gtWES lambda B and was subcloned into the plasmid vectors pUC18 and pUC19 in which xylanase activity was expressed in both orientations. The xylanase was located in the periplasmic space of the host, Escheri...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Lee JM,Hu Y,Zhu H,Cheng KJ,Krell PJ,Forsberg CW

    更新日期:1993-01-01 00:00:00

  • The microbial degradation of cyclohexanecarboxylic acid by a beta-oxidation pathway with simultaneous induction to the utilization of benzoate.

    abstract::The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Blakley ER

    更新日期:1978-07-01 00:00:00

  • Factors affecting rate of methane formation from acetic acid by enriched methanogenic cultures.

    abstract::A stable enrichment culture converting acetic acid to methane was successfully obtained from a pear waste digester, using a synthetic substrate solution with acetic acid as the main carbon source. This enrichment culture converted up to 10 mmol of acetic acid per litre per day at 35 degrees C and did not use hydrogen ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: van den Berg L,Patel GB,Clark DS,Lentz CP

    更新日期:1976-09-01 00:00:00

  • Isolation of a spermatozoal immobilization factor from Staphylococcus aureus filtrates.

    abstract::Staphylococcus aureus isolated from the cervix of an infertile woman was found to cause complete immobilization of human spermatozoa in vitro. Only the cell culture and cell-free supernatant showed immobilization activity, indicating that the sperm immobilization factor might be released extracellularly by the organis...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Prabha V,Gupta T,Kaur S,Kaur N,Kala S,Singh A

    更新日期:2009-07-01 00:00:00

  • Description of bacteria able to degrade isoquinoline in pure culture.

    abstract::Isoquinoline is a nitrogen heterocyclic compound that is associated with coal- and oil-derived wastes. Four strains of bacteria able to degrade isoquinoline in pure culture were isolated from sites known to be contaminated with oil. Isoquinoline was used as the sole source of carbon and nitrogen by these isolates. Iso...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Aislabie J,Richards NK,Lyttle TC

    更新日期:1994-07-01 00:00:00

  • Biosynthesis of epsilon-rhodomycinone from glucose by Streptomyces C5 and comparison with intermediary metabolism of other polyketide-producing streptomycetes.

    abstract::The catabolism of glucose by Streptomyces C5, a producer of anthracycline antibiotics, was investigated to determine the pathways that supply precursors for anthracycline biosynthesis. Carbons for the biosynthesis of epsilon-rhodomycinone, an anthracycline aglycone, from radiolabelled glucose were derived primarily fr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Dekleva ML,Strohl WR

    更新日期:1988-11-01 00:00:00

  • Polygalacturonase isolated from the culture of the psychrophilic fungus Sclerotinia borealis.

    abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Takasawa T,Sagisaka K,Yagi K,Uchiyama K,Aoki A,Takaoka K,Yamamato K

    更新日期:1997-05-01 00:00:00

  • Transcriptional regulation of histidine biosynthesis genes in Corynebacterium glutamicum.

    abstract::Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. The latter his operon starts the transcription at the C residue localized...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Jung S,Chun JY,Yim SH,Lee SS,Cheon CI,Song E,Lee MS

    更新日期:2010-02-01 00:00:00

  • Direct lymphocytotoxicity against herpes simplex virus infected cells.

    abstract::This study was undertaken to determine if direct cytotoxicity (DC) against herpes simplex virus infected cells, perhaps mediated by T cells, could be demonstrated in individuals subject to recurrent herpes labialis. The mononuclear cells from 7 out of 17 individuals with recurrent herpes expressed DC whereas no DC was...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Fujimiya Y,Babiuk LA,Rouse BT

    更新日期:1978-09-01 00:00:00

  • Hydrogen cyanide production by Pseudomonas aeruginosa at reduced oxygen levels.

    abstract::Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Castric PA

    更新日期:1983-10-01 00:00:00

  • An electroporation-based system for high-efficiency transformation of germinated conidia of filamentous fungi.

    abstract::A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Chakraborty BN,Patterson NA,Kapoor M

    更新日期:1991-11-01 00:00:00

  • Increased ultraviolet radiation sensitivity of Escherichia coli grown at low temperature.

    abstract::The repair of DNA damage caused by ultraviolet radiation (UVR) is well understood in both lower and higher organisms. Genetic studies carried out at optimum temperature for growth, 37 °C in Escherichia coli, have revealed the major pathways of DNA repair. We show that E. coli cells grown at 20 °C are more sensitive to...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Mangoli S,Rath D,Goswami M,Jawali N

    更新日期:2014-05-01 00:00:00

  • Regulation of peptidoglycan biosynthesis in relA+ and relA- strains of Escherichia coli during diauxic growth on glucose and lactose.

    abstract::In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Ishiguro EE

    更新日期:1979-10-01 00:00:00

  • Changes in glutathione metabolic enzymes during yeast-to-mycelium conversion of Candida albicans.

    abstract::Candida albicans is a dimorphic yeast capable of producing alternate morphological forms (yeast or mycelium) in response to environmental changes. The intracellular level of glutathione, which helps to maintain the redox potential of the cell, is decreased significantly during the thermal induction of yeast-to-myceliu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Manavathu M,Gunasekaran S,Porte Q,Manavathu E,Gunasekaran M

    更新日期:1996-01-01 00:00:00

  • Genomic comparison of facultatively anaerobic and obligatory aerobic Caldibacillus debilis strains GB1 and Tf helps explain physiological differences.

    abstract::Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Wushke S,Froese A,Fristensky B,Zhang XL,Spicer V,Krokhin OV,Levin DB,Sparling R

    更新日期:2019-06-01 00:00:00

  • Cytochrome aa3 in facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense.

    abstract::We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N', N'-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c o...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Nunoura T,Sako Y,Wakagi T,Uchida A

    更新日期:2005-08-01 00:00:00

  • Neutralizing response of rabbits to an experimental rubella subunit vaccine made from immunostimulating complexes.

    abstract::The purpose of this study was to evaluate experimentally the immunogenicity in rabbits of rubella subunits adsorbed to the adjuvant Quil A. The adsorbed viral proteins form structurally defined ImmunoStimulating COMplexes (ISCOMs). Rubella ISCOMs were tested for their capacity to induce neutralizing and hemagglutinati...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Trudel M,Nadon F,Séguin C,Payment P

    更新日期:1988-12-01 00:00:00

  • A potential role of Escherichia coli pathobionts in the pathogenesis of pediatric inflammatory bowel disease.

    abstract::Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Schippa S,Iebba V,Totino V,Santangelo F,Lepanto M,Alessandri C,Nuti F,Viola F,Di Nardo G,Cucchiara S,Longhi C,Conte MP

    更新日期:2012-04-01 00:00:00

  • BaeR protein acts as an activator of nuclear factor-kappa B and Janus kinase 2 to induce inflammation in murine cell lines.

    abstract::BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to ind...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Lee SJ,Birhanu BT,Awji EG,Kim MH,Park JY,Suh JW,Park SC

    更新日期:2016-09-01 00:00:00

  • Environmental scanning electron microscopic observation of the hyphal sheath and mycofibrils in Postia placenta.

    abstract::Environmental scanning electron microscopic observations of Postia placenta grown on a defined medium and on red spruce wood allowed for the examination of the hydrated sheath of P. placenta. In the wood environment, mature hyphae that were not adhering to the substrate were observed to have a mycofibrillar morphology...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Connolly JH,Chen Y,Jellison J

    更新日期:1995-04-01 00:00:00

  • Uridylylation of the PII protein from Herbaspirillum seropedicae.

    abstract::The PII protein is apparently involved in the control of NifA activity in Herbaspirillum seropedicae. To evaluate the probable role of PII in signal transduction, uridylylation assays were conducted with purified H. seropedicae PII and Escherichia coli GlnD, or a cell-free extract of H. seropedicae as sources of uridy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Benelli EM,Buck M,de Souza EM,Yates MG,Pedrosa FO

    更新日期:2001-04-01 00:00:00