Abstract：
:Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high percentage of similarity with known stress proteins. One protein match was the Fe superoxide dismutase (Fe-SOD), another was a tellurite resistance protein (Ter), the third was a bacterioferritin comigratory protein (Bcp); and two matches, differing only by their isoelectric point, were the same small heat shock protein (Hsp), a major immune reactive protein found in mycobacteria. This suggests that the symbiotic microorganism Frankia, first responds with a normal stress response to toxic root products of its symbiotic host plant. To confirm its identity, the gene corresponding to the Fe-SOD protein, sodF was isolated from a genomic library by a PCR-approach and sequenced. It is the first stress response gene characterized in Frankia.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Hammad Y,Maréchal J,Cournoyer B,Normand P,Domenach AMdoi
10.1139/w01-046subject
Has Abstractpub_date
2001-06-01 00:00:00pages
541-7issue
6eissn
0008-4166issn
1480-3275journal_volume
47pub_type
杂志文章abstract：:Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-153
更新日期：1978-08-01 00:00:00
abstract：:Escherichia coli is capable of producing several virulence factors involved in the pathophysiology of bovine mastitis. Cell surface structures such as flagella, pili, and other outer membrane adhesins are considered essential for biofilm formation on abiotic surfaces. The aim of this study was characterize the molecul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0053
更新日期：2013-05-01 00:00:00
abstract：:Listeria grayi is a nonpathogenic Gram-positive bacterium that demonstrates considerable similarities to other members in the genus Listeria, including the foodborne human pathogen Listeria monocytogenes and the animal pathogen Listeria ivanovii. A rapid diagnostic test to identify and diagnose listeriosis would be va...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-108
更新日期：2005-01-01 00:00:00
abstract：:Nitrogenase in Azotobacter chroococcum whole cells was inhibited by enzymically generated superoxide anion (O2-), hydrogen peroxide, and ethyl hydrogen peroxide. The degree of inhibition produced by O2- was related to the quantity of oxygen supplied to the organisms in continuous cultures. O2- also inhibited oxygen up...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-228
更新日期：1977-11-01 00:00:00
abstract：:Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-013
更新日期：2004-04-01 00:00:00
abstract：:The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to q...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:A total of 230 strains of metal-tolerant bacteria from water and sediment samples collected in Chesapeake Bay were isolated on a medium containing cobalt, lead, mercury, or molybdenum. In addition, a set of 71 cultures were simultaneously isolated on glucose tryptone yeast extract agar medium without metals. Twenty-th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-212
更新日期：1977-10-01 00:00:00
abstract：:Neisseria gonorrhoeae exist in transparent (Tr) and opaque (Op) colony forms. Op forms are recovered from patients early in the menstrual cycle; Tr colonies predominate late in the cycle. The mechanism for this colonial variation was examined by determining the influence of gonodal hormones on growth inhibition of Op ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-044
更新日期：1982-03-01 00:00:00
abstract：:A field site near Humboldt, Saskatchewan, was annually treated with hog or cattle manure and cropped to canola, spring wheat, barley, and canola from 1997 to 2000. During each growing season, soil was analyzed for microbial populations in terms of activity and community structure, and crops were assessed for root rot ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-069
更新日期：2003-09-01 00:00:00
abstract：:Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-083
更新日期：2012-09-01 00:00:00
abstract：:HPr is a protein of the phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In gram-positive bacteria, HPr can be phosphorylated on Ser-46 by the kinase/phosphorylase HprK/P and on His-15 by phospho-enzyme I (EI~P) of the PTS. In vitro studies with purified HPrs from Bacillus subtilis, Enterococcus fa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-085
更新日期：2008-11-01 00:00:00
abstract：:The monitoring of antimicrobial susceptibilities in Neisseria gonorrhoeae isolates and characterization of N. gonorrhoeae multiantigen sequence types (NG-MAST, ST) provide important surveillance data as resistance rates continue to rise. A total of 2970 N. gonorrhoeae isolates were collected by Canadian provincial pub...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0357
更新日期：2013-10-01 00:00:00
abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-129
更新日期：2002-01-01 00:00:00
abstract：:In Neurospora crassa, heat shock results in the induction of 9 to 11 heat shock proteins (HSP), of which HSP80 is the most abundant and the first to be synthesized. The induction of HSP80 was investigated during normal growth (2% sucrose) and under sucrose starvation. Transfer of mycelium to a medium supplemented with...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-031
更新日期：1988-02-01 00:00:00
abstract：:One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-029
更新日期：2001-05-01 00:00:00
abstract：:The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-033
更新日期：1992-03-01 00:00:00
abstract：:The antibacterial mechanism of a Cinnamomum cassia essential oil from Vietnam and of its main component (trans-cinnamaldehyde, 90% (m/m) of C. cassia essential oil) against a Listeria innocua strain was investigated to estimate their potential for food preservation. In the presence of C. cassia essential oil or trans-...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0481
更新日期：2015-04-01 00:00:00
abstract：:Staphylococcus aureus was grown in a rich peptone medium which became alkaline with continued incubation. Cells were grown at 37 degrees C and in the same medium containing 1 M NaCl at 46 degrees C, a temperature at which this organism can grow only when protected by NaCl. Cells of these cultures are hereafter called ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-049
更新日期：1983-03-01 00:00:00
abstract：:Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-079
更新日期：1978-04-01 00:00:00
abstract：:To facilitate the recognition of plant viruses with similar characteristics a dendrogram of characterized viruses was constructed. The sequence of criteria included: type of nucleic acid; single or double stranded; presence or absence of lipid envelope; helical or nonhelical symmetry; and divided or single genome. Non...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-197
更新日期：1977-09-01 00:00:00
abstract：:Viruses were isolated from every sample of raw (100 L) and treated (1000 L) water collected at a water treatment plant drawing sewage-contaminated river water. Few plaque-forming isolates were formed but cytopathogenic viruses were isolated as frequently in drinking water as in raw water. In drinking water some sample...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-063
更新日期：1981-04-01 00:00:00
abstract：:Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-099
更新日期：1991-08-01 00:00:00
abstract：:Aspergillus caespitosus NRRL 5769 growing in broth containing small amounts of sitosterol produced substance(s) with greater inhibitory activity against Candida albicans ATCC 10231 than in broth without sitosterol. Subsequent isolation, purification, and structural elucidation yielded 5,6-dihydro-5(S)-acetoxy-6(S)-1,2...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-259
更新日期：1975-11-01 00:00:00
abstract：:Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-039
更新日期：1987-03-01 00:00:00
abstract：:A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-027
更新日期：1993-02-01 00:00:00
abstract：:Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w07-115
更新日期：2008-01-01 00:00:00
abstract：:Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-148
更新日期：1976-07-01 00:00:00
abstract：:Samples of seawater, sediment, and the following invertebrates were collected from the Mediterranean Sea in the neighborhood of Alexandria, Egypt: sea urchins (Echinus spp.), clams (Tapes spp.), and wedge shells (Donax trunculus). A total of 165 samples were collected from November 1979 to July 1980. Average counts (p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-187
更新日期：1982-11-01 00:00:00
abstract：:Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-007
更新日期：2012-04-01 00:00:00
