Abstract：
:Escherichia coli is capable of producing several virulence factors involved in the pathophysiology of bovine mastitis. Cell surface structures such as flagella, pili, and other outer membrane adhesins are considered essential for biofilm formation on abiotic surfaces. The aim of this study was characterize the molecular profile of E. coli isolates obtained from mastitic milk by using the technique of pulsed-field gel electrophoresis (PFGE) and evaluating results within the pulsetypes formed with the presence of virulence genes involved in biofilm production. Twenty-seven E. coli biofilm-producing isolates were obtained from mastitic milk samples belonging to Brazilian Agricultural Research Corporation; these samples were analyzed by PFGE. We identified 9 (I to IX) different phylogenetic groups with a similarity of 90% and observed the presence of fimA (large subunit of the fimbriae type 1) and csgA gene (large subunit of curli fimbriae) in all isolates. Sixteen isolates had the gene fliC (flagellin). Evaluation of the genetic profile carried out through the PFGE showed that the bacteria do not share the same origin. It was not possible to associate the biofilm production capacity with the presence or absence of related genes. Therefore, strains of environmental mastitis-causing E. coli present different pathotypes.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Silva VO,Espeschit IF,Moreira MAdoi
10.1139/cjm-2013-0053subject
Has Abstractpub_date
2013-05-01 00:00:00pages
291-3issue
5eissn
0008-4166issn
1480-3275journal_volume
59pub_type
杂志文章abstract：:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...
journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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doi：
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更新日期：2012-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1139/m75-103
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pub_type： 杂志文章
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pub_type： 杂志文章
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