Abstract:
:Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modification of the other fractions was observed after infection.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Payment P,Ajdukovic D,Pavilanis Vdoi
10.1139/m75-103subject
Has Abstractpub_date
1975-05-01 00:00:00pages
718-22issue
5eissn
0008-4166issn
1480-3275journal_volume
21pub_type
杂志文章abstract::A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-059
更新日期:1997-05-01 00:00:00
abstract::A proline dehydrogenase which catalyzes the nicotinamide adenine dinucleotide (NAD) dependent oxidation of proline and the NADH-dependent reduction of delta 1-pyrroline 5-carboxylic acid (PCA) was purified from extracts of Clostridium sporogenes. Following purification, only one protein band was found on analytical po...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-147
更新日期:1981-09-01 00:00:00
abstract::Four phages isolated from cattle and poultry feces were analyzed for their ability to lyse Salmonella serotypes and Escherichia coli O157:H7. The phage one-step growth curves, morphology, and genetic characteristics were determined. All phages showed a lytic effect on various Salmonella serotypes and E. coli O157:H7, ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-099
更新日期:2011-12-01 00:00:00
abstract::Citrate synthase (citrate-oxaloacetate lyase (CoA acetylating), EC 4.1.3.7) has been purified to electrophoretic homogeneity from a marine Pseudomonas. The enzyme was made up of identical subunits, with a molecular wieght of about 53 000, as determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis. T...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-039
更新日期:1978-03-01 00:00:00
abstract::beta-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-146
更新日期:2009-04-01 00:00:00
abstract::Plant endophytes are a group of microorganisms that reside asymptomatically within the healthy living tissue. The diversity and molecular and biochemical characterization of industrial hemp-associated endophytes have not been previously studied. This study explored the abundance and diversity of culturable endophytes ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0108
更新日期:2018-10-01 00:00:00
abstract::The morphology of Clostridium pasteurianum cells grown on 10(-2) M SO32- showed significant alteration in cell shape and the absence of the electron translucent reserve polysaccharide (amylopectin) when compared to sulfate-grown cells. At the lower sulfite concentrations (10(-3) and 10(-4)M SO32-) the cells showed the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-036
更新日期:1976-02-01 00:00:00
abstract::The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-057
更新日期:1994-05-01 00:00:00
abstract::Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequenc...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-059
更新日期:1993-04-01 00:00:00
abstract::During exponential growth, strain AW405 of Escherichia coli K-12 did not regulate the fatty acid composition of its lipids in response either to temperature or to the addition of NaCl, KCl, or MgCl2 to the medium. Growth was severely restricted at temperatures below 21 degrees C. Differential scanning calorimetry (DSC...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-128
更新日期:1981-08-01 00:00:00
abstract::A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virol...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-186
更新日期:1975-08-01 00:00:00
abstract::The gene for a type IIo IgG-binding protein has previously been cloned and sequenced. The approximately 60,000 M(r) recombinant gene product binds all four human IgG subclasses and fibrinogen. Treatment of this recombinant protein with CNBr results in generation of a series of fragments. One fragment, an approximately...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-149
更新日期:1996-11-01 00:00:00
abstract::This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-001
更新日期:1983-01-01 00:00:00
abstract::Unicellular green alga represents not only a convenient model for its biochemical and physiological studies but also a sensitive system to test the effects of various environmental factors. Algae cells of two strains, SA-3 strain (exsymbiotic from Paramecium bursaria) and Chlorella vulgaris c-27, were asynchronously c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-143
更新日期:2002-02-01 00:00:00
abstract::A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-222
更新日期:1979-12-01 00:00:00
abstract::Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2020-0254
更新日期:2020-12-10 00:00:00
abstract::Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-099
更新日期:2004-12-01 00:00:00
abstract::Pili are essential adhesive determinants for many bacterial pathogens. A suppressor mutation screen that takes advantage of a pilus-mediated self-aggregative "hockey-puck" colony phenotype was designed to identify novel regulators of type I pili in Serratia marcescens. Mutations that decreased pilus biosynthesis mappe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0566
更新日期:2016-01-01 00:00:00
abstract::The effects of water activity (aw, 0.994-0.90 identical to 0.4-14.0 (-)MPa water potential), temperature (4-45 degrees C), and pH (3.6, 5.5, 7.0), and their interactions on growth of isolates of Fusarium moniliforme and Fusarium proliferatum were determined in vitro on a maize extract agar medium. Growth of two isolat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-149
更新日期:1995-12-01 00:00:00
abstract::BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to ind...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2016-0057
更新日期:2016-09-01 00:00:00
abstract::The rapid onset of resistance reduces the efficacy of most conventional antimicrobial drugs and is a general cause of concern for human well-being. Thus, there is great demand for a continuous supply of novel antibiotics to combat this problem. Bacteria-derived antimicrobial peptides (AMPs) have long been used as food...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/cjm-2014-0613
更新日期:2015-02-01 00:00:00
abstract::In an effort to reduce human exposure to adenoviruses through drinking water, we determined the effectiveness of sequential disinfection with an alternative ultraviolet (UV) technology (medium-pressure (MP) UV) and monochloramine. The results of this study showed that MP UV was much more effective than traditional UV ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-047
更新日期:2010-07-01 00:00:00
abstract::We examined factors related to the potent antagonistic effect of Escherichia coli and Bacteroides ovatus on Staphylococcus aureus in anaerobic continuous flow cultures. In the presence of sugars fermentable by E. coli alone or both E. coli and S. aureus, motile E. coli strains exerted a potent antagonistic effect and ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-106
更新日期:1988-05-01 00:00:00
abstract::In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Iron and sulfur oxidation by Thiobacillus ferrooxidans as well as growth on ferrous iron were inhibited by a variety of low molecular weight organic compounds. The influences of chemical structure of the organic inhibitors, pH, temperature, physical treatment of cells, and added inhibitory or stimulatory inorganic ion...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-105
更新日期:1976-05-01 00:00:00
abstract::The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-183
更新日期:1995-01-01 00:00:00
abstract::We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-064
更新日期:2006-11-01 00:00:00
abstract::Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-016
更新日期:1993-01-01 00:00:00
abstract::Klebsiella pneumoniae was found to effect the reductive dechlorination of methoxychlor (2,2-bis(p-methoxyphenyl)-1, 1, 1-trichloroethane) and its dihydroxy derivative (2, 2-bis (p-hydroxyphenyl)-1, 1, 1-trichloroethane) to dichloro derivatives 2, 2-bis (p-methoxyphenyl)-1, 1-dichloroethane and 2, 2-bis (p-hydroxypheny...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-023
更新日期:1982-02-01 00:00:00
abstract::Scanning and transmission electron microscopy was applied for a morphological study of three strains of Bifidobacterium grown on solid or liquid media. The pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. A possible sequence of the morphological events during transformatio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-197
更新日期:1975-09-01 00:00:00