Abstract：
:Pili are essential adhesive determinants for many bacterial pathogens. A suppressor mutation screen that takes advantage of a pilus-mediated self-aggregative "hockey-puck" colony phenotype was designed to identify novel regulators of type I pili in Serratia marcescens. Mutations that decreased pilus biosynthesis mapped to the fimABCD operon; to the genes alaT, fkpA, and oxyR; upstream of the flagellar master regulator operon flhDC; and to an uncharacterized gene encoding a predicted DUF1401 domain. Biofilm formation and pilus-dependent agglutination assays were used to characterize the relative importance of the identified genes in pilus biosynthesis. Additional mutagenic or complementation analysis was used to verify the role of candidate genes in pilus biosynthesis. Presented data support a model that CRP negatively regulates pilus biosynthesis through increased expression of flhDC and decreased expression of oxyR. Further studies are warranted to determine the mechanism by which these genes mediate pilus biosynthesis or function.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Shanks RM,Stella NA,Brothers KM,Polaski DMdoi
10.1139/cjm-2015-0566subject
Has Abstractpub_date
2016-01-01 00:00:00pages
83-93issue
1eissn
0008-4166issn
1480-3275journal_volume
62pub_type
杂志文章abstract：:Caulobacters are biofilm-forming members of the natural flora of soil and aquatic environments, which exhibit several characteristics that make them attractive for development of high surface area microbial bioreactors or biosensors. Although caulobacters are well characterized genetically, little is known about their...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:Two molecular forms of alpha-galactosidase (EC 3.2.1.22) synthesized constitutively by Bacillus stearothermophilus, strain AT-7, have been purified. alpha-Galactosidase I (with the substrate p-nitrophenyl alpha-D-galactopyranoside (PNPG)) has a pH optimum of 6 and half-life at 65 degrees C of > 2 h at low protein conc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-166
更新日期：1980-08-01 00:00:00
abstract：:The attachment of Bradyrhizobium sp. (Lupinus) strain MSDJ718 to excised roots of white lupin was examined. Maximal attachment occurred at early to middle log phases of bacterial growth. This binding was pH dependent, with an optimal value reached at 6.6. Irrespective of the culture age, the attachment was strongly af...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-035
更新日期：1996-03-01 00:00:00
abstract：:An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-042
更新日期：1976-02-01 00:00:00
abstract：:Cultures of Streptomyces peucetius often were observed to divert spontaneously from the production of anthracycline antibiotics to the excretion of acidic metabolites, whether grown in a defined medium or a nutrient-rich medium. The predominant excreted acidic metabolites were determined by high-performance liquid chr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-198
更新日期：1987-12-01 00:00:00
abstract：:Examination of immune complexes between cells of Streptococcus mutans and homologous antiserum by the techniques of thin-sectioning and freeze-etching revealed that the cells were embedded within and extensive matrix 80-90 nm thick with defined boundaries. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-129
更新日期：1976-06-01 00:00:00
abstract：:The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-155
更新日期：1996-12-01 00:00:00
abstract：:Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-002
更新日期：2007-04-01 00:00:00
abstract：:Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-024
更新日期：1980-02-01 00:00:00
abstract：:Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-083
更新日期：2012-09-01 00:00:00
abstract：:A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-162
更新日期：1997-12-01 00:00:00
abstract：:The study of the relationship between MVL 51 virus, of the Gourlay group L 1, and the Mycoplasma cells of A. laidlawii during the first 35 min of infection leads to the conclusion that a "carrier state" has been established, free virus as well as infected and noninfected cells being present at the same time in the cul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:The peptidoglycan of Escherichia coli K-12 strain AB264 was isolated by treating whole cells with sodium dodecyl sulfate and was purified by deoxyribonuclease, ribonuclease, and trypsin treatment. Like the peptidoglycan of Bacillus subtilis, this peptidoglycan proved able to bind substantial amounts of metallic ions f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-031
更新日期：1984-02-01 00:00:00
abstract：:To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...
journal_title：Canadian journal of microbiology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1139/m94-128
更新日期：1994-10-01 00:00:00
abstract：:Numbers of viable bacteria as indicated by colony-forming units (CFU), microbial adenosine triphosphate levels, dissolved organic carbon (DOC), particulate organic carbon and nitrogen (POC and PON), glucose concentration, heterotrophic potential (Vmax), and in situ heterotrophic activity (Vn) of near-surface microlaye...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-251
更新日期：1976-12-01 00:00:00
abstract：:Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
abstract：:Wild-type glucose uptake and glucose chemotaxis activities were restored in glucose transport defective Pseudomonas aeruginosa strains PFB360 and PFB362 after introduction of plasmid pPZ129, containing a 1.1-kilobase DNA fragment that is essential for the expression of the P. aeruginosa periplasmic glucose binding pro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-163
更新日期：1993-11-01 00:00:00
abstract：:Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0254
更新日期：2020-12-10 00:00:00
abstract：:The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-105
更新日期：2007-12-01 00:00:00
abstract：:Spiroplasmas were cultured from nonsurface-sterilized flowers of magnolia tree (Magnolia grandiflora L.) and tulip tree (Liriodendron tulipfera L.) in Alameda, Solano, and Yolo counties in California. Spiroplasmas were also isolated from honey bees (Apis mellifera L.) collected in the vicinity of Davis and Berkeley, C...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-038
更新日期：1981-02-01 00:00:00
abstract：:A strain of Pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. A CoA-mediated beta-oxidation pathway was induced for the metabolism of the compound. The organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-198
更新日期：1982-12-01 00:00:00
abstract：:Methanospirillum hungatei strains GP1 and JF1 when exposed to the adenosinetriphosphatase inhibitor N,N'-dicyclohexylcarbodiimide experienced a marked decline in growth rates, methane synthesis activities, and intracellular ATP concentrations. Although growth was inhibited, the intracellular ATP concentrations of all ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-146
更新日期：1982-08-01 00:00:00
abstract：:Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-062
更新日期：2006-10-01 00:00:00
abstract：:Seven streptomycete strains were tested for biotransformation of salicylate. The products were identified by nuclear magnetic resonance spectroscopy and three types of conversion were found. Streptomyces cinnamonensis and Streptomyces spectabilis formed gentisate and salicylamide concurrently. Streptomyces rimosus tra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-111
更新日期：1996-08-01 00:00:00
abstract：:The phototrophic purple sulfur bacterium Thiopedia rosea forms multicellular, gas-vacuolate, regular, flat aggregates (platelets, sheets) held together by slime. Platelets found in eutrophic water consisted of slime (85% of the total wet volume) and 16 cells, while the gas-filled vacuole occupied 44% of the volume of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-113
更新日期：1986-07-01 00:00:00
abstract：:A proline dehydrogenase which catalyzes the nicotinamide adenine dinucleotide (NAD) dependent oxidation of proline and the NADH-dependent reduction of delta 1-pyrroline 5-carboxylic acid (PCA) was purified from extracts of Clostridium sporogenes. Following purification, only one protein band was found on analytical po...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-147
更新日期：1981-09-01 00:00:00
abstract：:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-056
更新日期：1992-04-01 00:00:00
abstract：:Roots of field-grown tropical maize, Panicum maximum Jacq. and Digitaria decumbens Stent., and of sorghum and wheat grown in monoxenic culture with the diazotroph Spirillum lipoferum (syn. Azospirillum spp.) were examined for tetrazolium-reducing bacteria following incubation of roots in a malate-phosphate buffer-2,3,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-122
更新日期：1978-06-01 00:00:00
abstract：:Microsclerotia of three melanin-deficient mutants of Verticillium dahliae formed malanin from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. The melanins formed from (+)-scytalone or 1,8-dihydroxynaphthalene resembled wild-type melanin chemically and ultrastructurally, whereas the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-049
更新日期：1978-03-01 00:00:00
