Abstract：
:Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as a single band of M(r) 66 kDa. The cytosolic chitinase from the mycelial extracts of these fungi was separated by heat treatment, ammonium sulphate precipitation, and by affinity chromatography with regenerated chitin. SDS-PAGE showed two bands for each fungus with M(r) of 69.5 and 55 kDa in C. cucurbitarum and M(r) 69.5 and 53 kDa in Ph. articulosus. Chitinases, membrane bound or cytosolic, hydrolyzed regenerated chitin, colloidal chitin, glycol chitin, N,N'-diacetylchitobiose, and N,N',N"-triacetylchitotriose. Heavy metals, inhibitors, and N-acetylglucosamine inhibited chitinase activity, whereas trypsin and an acid protease enhanced its activity. Chitinase preparations showed lysozyme activity that was inhibited by histamine but not by N-acetylglucosamine. There was no N-acetylglucosamanidase activity, but beta-1,3 glucanase activity was found in cytosolic preparations only. Despite slight differences in their molecular mass, both the membrane-bound and cytosolic chitinases showed similarities in substrate utilization, response to inhibitors, and activation by trypsin and acid protease; pH and temperature optima also were similar.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Balasubramanian R,Manocha MSdoi
10.1139/m92-056subject
Has Abstractpub_date
1992-04-01 00:00:00pages
331-8issue
4eissn
0008-4166issn
1480-3275journal_volume
38pub_type
杂志文章abstract：:To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...
journal_title：Canadian journal of microbiology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1139/m94-128
更新日期：1994-10-01 00:00:00
abstract：:An investigation of brucellosis caused by Brucella suis, type 4, in reindeer, Rangifer tarandus L., and other ungulates inoculated experimentally with virulent isolates was undertaken to observe the course of infection, follow titres of serum agglutins, and determine the extent to which intraspecific and interspecific...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-024
更新日期：1978-02-01 00:00:00
abstract：:Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-020
更新日期：1988-02-01 00:00:00
abstract：:Samples of seawater, sediment, and the following invertebrates were collected from the Mediterranean Sea in the neighborhood of Alexandria, Egypt: sea urchins (Echinus spp.), clams (Tapes spp.), and wedge shells (Donax trunculus). A total of 165 samples were collected from November 1979 to July 1980. Average counts (p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-187
更新日期：1982-11-01 00:00:00
abstract：:Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-236
更新日期：1978-12-01 00:00:00
abstract：:Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-038
更新日期：2004-08-01 00:00:00
abstract：:The effect of hydrogen peroxide on Treponema pallidum was investigated. The in vitro loss of virulence (as measured by rabbit inoculation) of T. pallidum was accelerated by as low as 100 microM hydrogen peroxide in the complex maintenance medium used. Higher doses led to rapidly accelerated death with 500 microM hydro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-234
更新日期：1984-12-01 00:00:00
abstract：:Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-094
更新日期：2000-11-01 00:00:00
abstract：:To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0755
更新日期：2014-03-01 00:00:00
abstract：:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:The aim of this study was to determine the effect of the rhizosphere of maize on the diversity of denitrifying bacteria. Community structure comparison was performed by constructing a collection of isolates recovered from bulk and maize planted soil. A total of 3240 nitrate-reducing isolates were obtained and 188 of t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-037
更新日期：2004-07-01 00:00:00
abstract：:There are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. Fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. Through surveys of four bathing beaches on Lake Ontario, it w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-159
更新日期：1979-09-01 00:00:00
abstract：:Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-070
更新日期：2008-09-01 00:00:00
abstract：:The origin of the eukaryotic nucleus is difficult to reconstruct. While eukaryotic organelles (chloroplast, mitochondrion) are eubacterial endosymbionts, the source of nuclear genes has been obscured by multiple nucleotide substitutions. Using evolutionary parsimony, a newly developed rate-invariant treeing algorithm,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-017
更新日期：1989-01-01 00:00:00
abstract：:Proteins were solubilized from cell wall fractions of Candida albicans and separated by polyacrylamide gel electrophoresis. Cell walls were isolated from 25 and 37 degrees C growing and stationary phase yeast cultures and from germ tubes. The 42 protein bands detected by dye binding were observed in all wall extracts,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-220
更新日期：1983-10-01 00:00:00
abstract：:Phialocephala fortinii is a dark septate fungal endophyte that colonizes roots of many host species. Its effect on plant growth varies from being pathogenic to beneficial. The basic biology of this species has received little research, and thus the main objectives of this study were to determine cytological features o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-018
更新日期：2006-07-01 00:00:00
abstract：:The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-090
更新日期：1990-07-01 00:00:00
abstract：:HPr is a protein of the phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In gram-positive bacteria, HPr can be phosphorylated on Ser-46 by the kinase/phosphorylase HprK/P and on His-15 by phospho-enzyme I (EI~P) of the PTS. In vitro studies with purified HPrs from Bacillus subtilis, Enterococcus fa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-085
更新日期：2008-11-01 00:00:00
abstract：:In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-188
更新日期：1979-10-01 00:00:00
abstract：:Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-218
更新日期：1977-10-01 00:00:00
abstract：:We modified a technique, cDNA-AFLP, for identifying differentially expressed genes in plants to work in the filamentous fungus Leptosphaeria maculans (Desmaz.) Ces. & De Not. The cDNA fragments generated by our method ranged in size from approximately 100 to 400 bps. On average, twice as many cDNA fragments were ampli...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-100
更新日期：2001-10-01 00:00:00
abstract：:In the divE mutant, which has a temperature-sensitive mutation in the tRNA1(Ser) gene, the synthesis of beta-galactosidase is dramatically decreased at the non-permissive temperature. In Escherichia coli, the UCA codon is only recognized by tRNA1(Ser). Several genes containing UCA codons are normally expressed at 42 d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-deriv...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0485
更新日期：2014-12-01 00:00:00
abstract：:Morphological differentiation has commanded attention for its putative impact on the pathogenesis of invasive fungal infections. We evaluated in vitro and in vivo the dimorphism from mycelial to yeast-phase of Sporothrix schenckii, Blastomyces dermatitidis and Paracoccidioides brasiliensis isolates, two strains for ea...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-026
更新日期：2004-06-01 00:00:00
abstract：:The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-077
更新日期：2007-09-01 00:00:00
abstract：:We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-059
更新日期：1988-03-01 00:00:00
abstract：:Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-051
更新日期：1982-03-01 00:00:00
abstract：:Heat activation (70 degrees C for 20 min) resulted in alteration in structural proteins and enzymes found in Bacillus cereus spore coats. The three notable changes were increased glycosylation of coat proteins, alteration in polypeptide pattern on sodium dodecyl sulfate - polyacrylamide gels, and an increase in free S...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-062
更新日期：1981-04-01 00:00:00
abstract：:The susceptibility of congenitally immunodeficient mice to a nonencapsulated strain of Cryptococcus neoformans (strain M7) was evaluated. Gnotobiotic mice with defined congenital defects in innate immunity (beige) or cell-mediated immunity (athymic) or with combined defects in innate and cellular immunity (beige athym...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-144
更新日期：1991-11-01 00:00:00
