Use of lactose to induce expression of soluble NifA protein domains of Herbaspirillum seropedicae in Escherichia coli.

abstract：:Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...

journal_title：Canadian journal of microbiology

authors： Platt MW,Correa N Jr,Mold C

更新日期：1994-02-01 00:00:00

abstract：:The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the st...

journal_title：Canadian journal of microbiology

authors： Laguerre M,Turcotte R

更新日期：1975-12-01 00:00:00

abstract：:Various soil fungi were tested for their capacity to degrade the insecticide [14C]photodieldrin. Of nine species investigated, Trichoderma viride was the only one which degraded the insecticide to an appreciable extent into water-soluble, non-insecticidal compounds within 4-5 weeks. These products amounted to 32-41% o...

journal_title：Canadian journal of microbiology

authors： Tabet JC,Lichtenstein EP

更新日期：1976-09-01 00:00:00

abstract：:Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by q...

journal_title：Canadian journal of microbiology

authors： Burt WR,Smith RA

更新日期：1983-01-01 00:00:00

abstract：:The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...

journal_title：Canadian journal of microbiology

authors： Gilchrist A,Fisher JA,Smit J

更新日期：1992-03-01 00:00:00

abstract：:Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.3...

journal_title：Canadian journal of microbiology

authors： Nath K,Kumar A,Das D

更新日期：2006-06-01 00:00:00

abstract：:The environmental distribution of Giardia spp. and Cryptosporidium spp. is dependent upon human, agricultural, and wildlife sources. The significance of each source with regard to the presence of parasites in the environment is unknown. This 2-year study examined parasite prevalence in human sewage influent, wildlife,...

journal_title：Canadian journal of microbiology

authors： Heitman TL,Frederick LM,Viste JR,Guselle NJ,Morgan UM,Thompson RC,Olson ME

更新日期：2002-06-01 00:00:00

abstract：:Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...

journal_title：Canadian journal of microbiology

authors： Gil-Agudelo DL,Fonseca DP,Weil E,Garzón-Ferreira J,Smith GW

更新日期：2007-04-01 00:00:00

abstract：:The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...

journal_title：Canadian journal of microbiology

authors： Mercier J,Jiménez JI

更新日期：2007-03-01 00:00:00

abstract：:A transformation protocol mediated by Agrobacterium tumefaciens is described for the sapstaining fungus Ophiostoma piceae. We compared transformants obtained from Agrobacterium with those obtained from yeast-like cells made into spheroplasts and treated with CaCl2. For all putative transformants analyzed, Southern hyb...

journal_title：Canadian journal of microbiology

authors： Tanguay P,Breuil C

更新日期：2003-04-01 00:00:00

abstract：:Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...

journal_title：Canadian journal of microbiology

authors： Hendry AT

更新日期：1983-10-01 00:00:00

abstract：:Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive...

journal_title：Canadian journal of microbiology

authors： Bruver RM,Ball JS,Tustanoff ER

更新日期：1975-06-01 00:00:00

abstract：:Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these ogranisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. This organism requires salts of seawater for both growth and extracell...

journal_title：Canadian journal of microbiology

authors： Maeda M,Taga N

更新日期：1976-10-01 00:00:00

abstract：:The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown...

journal_title：Canadian journal of microbiology

authors： Abelovska L,Bujdos M,Kubova J,Petrezselyova S,Nosek J,Tomaska L

更新日期：2007-04-01 00:00:00

abstract：:Growth rates and cell yields of Mycobacterium convolutum strain R22 grown on a wide range of both odd- and even-numbered carbon solid n-alkanes decreased as the substrate carbon number increased. Cellular lipid was 2.5 times higher following growth on the hydrocarbon substrates. The amount of polar lipid was found to ...

journal_title：Canadian journal of microbiology

authors： Hallas LE,Vestal JR

更新日期：1978-10-01 00:00:00

abstract：:Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...

journal_title：Canadian journal of microbiology

authors： Fons F,Amellal N,Leyval C,Saint-Martin N,Henry M

更新日期：2003-06-01 00:00:00

abstract：:In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of ...

journal_title：Canadian journal of microbiology

authors： Slížová M,Nemcová R,Mad'ar M,Hadryová J,Gancarčíková S,Popper M,Pistl J

更新日期：2015-06-01 00:00:00

abstract：:Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...

journal_title：Canadian journal of microbiology

authors： Larouche É,Généreux M,Tremblay MÈ,Rhouma M,Gasser MO,Quessy S,Côté C

更新日期：2020-10-01 00:00:00

abstract：:The nucleotide sequence of the murE gene encoding the diaminopimelic acid adding enzyme of Escherichia coli is reported. The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs. The deduced primary structure of MurE comprised 471 amino acid re...

journal_title：Canadian journal of microbiology

authors： Tao JS,Ishiguro EE

更新日期：1989-11-01 00:00:00

abstract：:Rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. Knowledge about the indigenous population is necessary for the selection and application of inoculant strains. In this study, we have assessed the genetic diversity of Bradyrhizobium strains isolated...

journal_title：Canadian journal of microbiology

authors： Saleena LM,Loganathan P,Rangarajan S,Nair S

更新日期：2001-02-01 00:00:00

abstract：:Two bacterial species, Gram-negative Escherichia coli and Gram-positive Bacillus subtilis, were exposed to different auxins to examine possible effects of these substances on bacterial stress tolerance. Bacterial resistance to UV irradiation, heat shock, and streptomycin was assessed with and without previous exposure...

journal_title：Canadian journal of microbiology

authors： Repar J,Šućurović S,Zahradka K,Zahradka D,Ćurković-Perica M

更新日期：2013-11-01 00:00:00

abstract：:Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...

journal_title：Canadian journal of microbiology

authors： Prado SM,Cerdán ME,González Siso MI

更新日期：2004-08-01 00:00:00

abstract：:The results of sucrose-density-gradient centrifugation of a cell-free particulate enzyme system from Acetobacter xylinum which was incubated with uridine diphosphoglucose indicate that there is a polymeric intermediate in the biosynthesis of cellulose. This intermediate has the properties of an oligomer of glucose, is...

journal_title：Canadian journal of microbiology

authors： Kjosbakken J,Colvin JR

更新日期：1975-02-01 00:00:00

abstract：:The physiochemical characteristics of the intracellular proteolytic enzymes of Oidiodendron kalari, a neuropathogenic fungus, were studied. The organism in the yeast phase was grown in a semisynthetic medium containing 1% tryptone, at 37 degrees C for 48 hr, on a gyrotory shaker. The crude extract was prepared by brea...

journal_title：Canadian journal of microbiology

authors： Cino PM,Tewari RP

更新日期：1975-09-01 00:00:00

abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...

journal_title：Canadian journal of microbiology

authors： White AP,Allen-Vercoe E,Jones BW,DeVinney R,Kay WW,Surette MG

更新日期：2007-01-01 00:00:00

abstract：:When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...

journal_title：Canadian journal of microbiology

authors： Ncango DM,Swart CW,Goldblatt ME,Pohl CH,Van Wyk PW,Botes PJ,Kock JL

更新日期：2008-06-01 00:00:00

abstract：:Isoquinoline is a nitrogen heterocyclic compound that is associated with coal- and oil-derived wastes. Four strains of bacteria able to degrade isoquinoline in pure culture were isolated from sites known to be contaminated with oil. Isoquinoline was used as the sole source of carbon and nitrogen by these isolates. Iso...

journal_title：Canadian journal of microbiology

authors： Aislabie J,Richards NK,Lyttle TC

更新日期：1994-07-01 00:00:00

abstract：:HI plasmids are distinguished by their thermosensitive mode of conjugation (transfer efficiency is optimal at 22-30 degrees C) and their capacity to encode multiple antibiotic resistance. These traits have implicated HI plasmids as potential vectors in the dissemination of antibiotic resistance among pathogenic and in...

journal_title：Canadian journal of microbiology

authors： Maher D,Taylor DE

更新日期：1993-06-01 00:00:00

abstract：:Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...

journal_title：Canadian journal of microbiology

authors： Tershak DR

更新日期：1985-12-01 00:00:00

abstract：:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...

journal_title：Canadian journal of microbiology

authors： Ogg JE,Lee SY,Ogg BJ

更新日期：1979-09-01 00:00:00