Growth responses of Neisseria gonorrhoeae auxotypes to required amino acids and bases in liquid medium.

Abstract:

:Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other strains gave indeterminate responses on agar and the reasons were not obvious. Liquid growth studies for quantifying the usual responses showed that yields of appropriate N. gonorrhoeae auxotrophs were proportional to replacement concentrations of any one of these amino acids or bases, of methionine, or of cysteine plus cystine. This type of response, where log growth rates and lag times were unaffected, is proposed as the basis for defining (simple) auxotrophy in gonococci. The formula of the defined medium was improved by increasing proline, uracil, and hypoxanthine beyond limiting concentrations, and decreasing citrulline or ornithine, and cysteine plus cystine. Fatty acid--free bovine albumin was used to ensure homogeneous growth in liquid media. In agar, it was superior to starch for the nonnutritive protective effect required by many strains.

journal_name

Can J Microbiol

authors

Hendry AT

doi

10.1139/m83-204

subject

Has Abstract

pub_date

1983-10-01 00:00:00

pages

1309-13

issue

10

eissn

0008-4166

issn

1480-3275

journal_volume

29

pub_type

杂志文章
  • Molecular techniques and their potential application in monitoring the microbiological quality of indoor air.

    abstract::Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章,评审

    doi:10.1139/m95-091

    authors: MacNeil L,Kauri T,Robertson W

    更新日期:1995-08-01 00:00:00

  • Soil microbial populations, community composition, and activity as affected by repeated applications of hog and cattle manure in eastern Saskatchewan.

    abstract::A field site near Humboldt, Saskatchewan, was annually treated with hog or cattle manure and cropped to canola, spring wheat, barley, and canola from 1997 to 2000. During each growing season, soil was analyzed for microbial populations in terms of activity and community structure, and crops were assessed for root rot ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-069

    authors: De Freitas JR,Schoenau JJ,Boyetchko SM,Cyrenne SA

    更新日期:2003-09-01 00:00:00

  • Chromosomal bacterial type II toxin-antitoxin systems.

    abstract::Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章,评审

    doi:10.1139/w2012-025

    authors: Syed MA,Lévesque CM

    更新日期:2012-05-01 00:00:00

  • Cytosolic and membrane-bound chitinases of two mucoraceous fungi: a comparative study.

    abstract::Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-056

    authors: Balasubramanian R,Manocha MS

    更新日期:1992-04-01 00:00:00

  • Protection of mammalian cells from diphtheria toxin by exogenous nucleotides.

    abstract::Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-046

    authors: Middlebrook JL,Dorland RB

    更新日期:1979-03-01 00:00:00

  • A single-bottle blood culture system: evaluation and comparison with two other systems.

    abstract::A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m77-155

    authors: Qadri SM,Wende RD

    更新日期:1977-08-01 00:00:00

  • Effect of some environmental parameters on fermentative hydrogen production by Enterobacter cloacae DM11.

    abstract::Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.3...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w06-005

    authors: Nath K,Kumar A,Das D

    更新日期:2006-06-01 00:00:00

  • Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution.

    abstract::Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and ot...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-44-11-1066

    authors: Bahirathan M,Puente L,Seyfried P

    更新日期:1998-11-01 00:00:00

  • Effect of calcium and anaerobiosis on the thermostability of Bacillus stearothermophilus.

    abstract::Bacillus stearothermophilus NCA 2184 lost viability and subsequently released cytoplasmic components when suspended in 0.1 M tris(hydroxymethyl)aminomethane (Tris) buffer (pH 7.2) and incubated at 60 degrees C. Cell lysis was prevented by the addition of 10 mM CaCl2 to the Tris-buffer suspension. Cells which were incu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-073

    authors: Mosley GA,Card GL,Koostra WL

    更新日期:1976-04-01 00:00:00

  • Improving the culturability of freshwater bacteria using FW70, a low-nutrient solid medium amended with sodium pyruvate.

    abstract::Bacterial culture based on the use of plate media is an effective method for investigating bacterial populations in the environment. To improve the culturability of bacteria from freshwater lakes, we developed a new medium, FW70, which contains sodium pyruvate, casamino acids, and lake water and is solidified using ge...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-019

    authors: Imazaki I,Kobori Y

    更新日期:2010-04-01 00:00:00

  • Evidence-based validation of quorum quenching from Lysinibacillus sphaericus and Geobacillus sp. in bioremediation of oil sludge.

    abstract::Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consorti...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2016-0323

    authors: Gómez-Garzón C,Dussán J

    更新日期:2017-01-01 00:00:00

  • The presence of Listeria spp. in raw milk in Ontario.

    abstract::Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-020

    authors: Farber JM,Sanders GW,Malcolm SA

    更新日期:1988-02-01 00:00:00

  • Copper stress and filamentous fungus Humicola lutea 103 - ultrastructural changes and activities of key metabolic enzymes.

    abstract::Humicola lutea 103 is a copper-tolerant fungal strain able to grow in the presence of 300 μg·mL(-1) Cu(2+) under submerged cultivation. To prevent the consequences of copper overload, microorganisms have evolved molecular mechanisms that regulate its uptake, intracellular traffic, storage, and efflux. In spite of this...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-112

    authors: Krumova ETs,Stoitsova SR,Paunova-Krasteva TS,Pashova SB,Angelova MB

    更新日期:2012-12-01 00:00:00

  • Extracellular nuclease produced by a marine bacterium. I. Extracellular deoxyribonuclease formation by a marine Vibrio sp.

    abstract::Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these ogranisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. This organism requires salts of seawater for both growth and extracell...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-213

    authors: Maeda M,Taga N

    更新日期:1976-10-01 00:00:00

  • The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuary.

    abstract::The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cyto...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2012-0606

    authors: Pereira Md,Byrne BA,Nguyen TB,Lewis DJ,Atwill ER

    更新日期:2013-06-01 00:00:00

  • Chemical and serological properties of a cyanogen bromide peptide of southern bean mosaic virus protein.

    abstract::Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-241

    authors: Tremaine JH,Ronald WP,Kelly EM

    更新日期:1980-12-01 00:00:00

  • Spatial distribution of bacterial communities in sediment of a eutrophic lake revealed by denaturing gradient gel electrophoresis and multivariate analysis.

    abstract::Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W08-098

    authors: Zeng J,Yang LY,Liang Y,Li JY,Xiao L,Jiang LJ,Zhao DY

    更新日期:2008-12-01 00:00:00

  • Yeast-like growth of Mucor alternans (van Tieghem) in tissue-culture medium 199.

    abstract::Mucor alternans(van Tieghem) was found to be a diphasic species in that it grew exclusively in the yeast-like budding phase under anaerobic conditions in the complex medium yeast extract-peptone-glucose broth and in tissue-culture medium 199. In the latter medium this growth form occurred also at 37C, at an initial pH...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-010

    authors: Leyritz M,Kapica L

    更新日期:1975-01-01 00:00:00

  • Urease activity related to the growth and differentiation of swarmer cells of Proteus mirabilis.

    abstract::Urease activity was measured using whole cells of both long (swarming) and short (nonswarming) populations of Proteus mirabilis from casein hydrolysate agar (CHA) and broth (CHB) cultures, and from brain heart infusion broth (BHIB) cultures. Urease is a constitutive enzyme for both long and short cells, but its activi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m87-051

    authors: Jin T,Murray RG

    更新日期:1987-04-01 00:00:00

  • Incidence of Norwalk virus infections during a prospective epidemiological study of drinking water-related gastrointestinal illness.

    abstract::To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...

    journal_title:Canadian journal of microbiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1139/m94-128

    authors: Payment P,Franco E,Fout GS

    更新日期:1994-10-01 00:00:00

  • Modulation of carbohydrate-binding capacities and attachment ability of Bradyrhizobium sp. (lupinus) to white lupin roots.

    abstract::The attachment of Bradyrhizobium sp. (Lupinus) strain MSDJ718 to excised roots of white lupin was examined. Maximal attachment occurred at early to middle log phases of bacterial growth. This binding was pH dependent, with an optimal value reached at 6.6. Irrespective of the culture age, the attachment was strongly af...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-035

    authors: Wisniewski JP,Delmotte FM

    更新日期:1996-03-01 00:00:00

  • Bactericidal activity of magainin 2: use of lipopolysaccharide mutants.

    abstract::Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m90-102

    authors: Macias EA,Rana F,Blazyk J,Modrzakowski MC

    更新日期:1990-08-01 00:00:00

  • Amino acid uptake systems in Bacteroides ruminicola.

    abstract::Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-180

    authors: Stevenson RM

    更新日期:1979-10-01 00:00:00

  • An efficient system for markerless gene replacement applicable in a wide variety of enterobacterial species.

    abstract::We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w06-102

    authors: White AP,Allen-Vercoe E,Jones BW,DeVinney R,Kay WW,Surette MG

    更新日期:2007-01-01 00:00:00

  • The effect of temperature and other growth conditions on the fatty acid composition of Escherichia coli.

    abstract::During exponential growth, strain AW405 of Escherichia coli K-12 did not regulate the fatty acid composition of its lipids in response either to temperature or to the addition of NaCl, KCl, or MgCl2 to the medium. Growth was severely restricted at temperatures below 21 degrees C. Differential scanning calorimetry (DSC...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-128

    authors: McGarrity JT,Armstrong JB

    更新日期:1981-08-01 00:00:00

  • Physical, chemical, and microbiological characteristics of microbial mats (kopara) in the South Pacific atolls of French Polynesia.

    abstract::Microbial mats that develop in shallow brackish and hyposaline ponds in the rims of two French polynesian atolls (Rangiroa and Tetiaroa) were intensively investigated during the past three years. Comparative assessment of these mats (called kopara in polynesian language) showed remarkable similarities in their composi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Che LM,Andréfouët S,Bothorel V,Guezennec M,Rougeaux H,Guezennec J,Deslandes E,Trichet J,Matheron R,Le Campion T,Payri C,Caumette P

    更新日期:2001-11-01 00:00:00

  • Nucleotide sequence of the murE gene of Escherichia coli.

    abstract::The nucleotide sequence of the murE gene encoding the diaminopimelic acid adding enzyme of Escherichia coli is reported. The coding region consisted of 1413 base pairs and was separated from the ftsI (penicillin-binding protein 3) gene by 61 base pairs. The deduced primary structure of MurE comprised 471 amino acid re...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-175

    authors: Tao JS,Ishiguro EE

    更新日期:1989-11-01 00:00:00

  • Escherichia coli inactivation mechanism by pressurized CO2.

    abstract::The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w06-078

    authors: Oulé MK,Tano K,Bernier AM,Arul J

    更新日期:2006-12-01 00:00:00

  • Overproduction of the Bacillus subtilis glutamyl-tRNA synthetase in its host and its toxicity to Escherichia coli.

    abstract::The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Pelchat M,Lacoste L,Yang F,Lapointe J

    更新日期:1998-04-01 00:00:00

  • Chemicals which promote survival of ultraviolet-irradiated lon and ruv mutants of Escherichia coli K12.

    abstract::The effects of postirradiation incubation with various chemicals structurally related to pantolactone on the survival of ultraviolet-irradiated Escherichia coli lon and ruv mutants were examined. Certain cyclic compounds with a butyrolactone, tetrahydrofuran, furan, tetrahydropyran, or 2-pyron ring showed substantial ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-254

    authors: Kato Y,Shiraki S,Hamada Y,Iyehara-Ogawa H,Nakayama H

    更新日期:1980-12-01 00:00:00