Abstract:
:Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline were taken up and incorporated into cold trichloroacetic acid precipitable material. Different patterns of incorporation and different responses to 2,4-dinitrophenol and potassium ferricyanide indicated multiple uptake systems were involved. Kinetic inhibition patterns suggested six distinct systems were present for amino acid uptake, with specificities related to the chemical structures of the amino acids. Thus, the failure of free amino acids to act as sole nitrogen sources for growth of B. ruminicola is not due to the absence of transport systems for these compounds.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Stevenson RMdoi
10.1139/m79-180subject
Has Abstractpub_date
1979-10-01 00:00:00pages
1161-8issue
10eissn
0008-4166issn
1480-3275journal_volume
25pub_type
杂志文章abstract::A moderately halophilic Acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. Maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2M NaCl or 1M KCl. Two kinds of amylase, amylases I and II were purified from the culture fil...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-169
更新日期:1978-09-01 00:00:00
abstract::The antimicrobial activity of Ag, Cu, and layered Ag-Cu surface films, sputter-coated onto several types of catheter material, against clinical isolates of Staphylococcus epidermidis and Staphylococcus aureus was evaluated. When 20 microL of a suspension of S. epidermidis or S. aureus (2.68 x 10(6) colony-forming unit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-134
更新日期:1993-09-01 00:00:00
abstract::The aim of this study was to assess the influence of sodium content on the microbiota on the surface of ready-to-eat (RTE) meat products purchased from the retail market in Canada. Products, including sliced and sausage-type deli meats, were analysed with culture-dependent and culture-independent methods. Bacteria wer...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0630
更新日期:2015-02-01 00:00:00
abstract::Scanning and transmission electron microscopy was applied for a morphological study of three strains of Bifidobacterium grown on solid or liquid media. The pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. A possible sequence of the morphological events during transformatio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-197
更新日期:1975-09-01 00:00:00
abstract::The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-119
更新日期:1990-10-01 00:00:00
abstract::Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0612
更新日期:2013-06-01 00:00:00
abstract::Vero cells were more suitable then BHK-21 cells for the detection of rubella-specific IgG antibodies because the nonspecific fluorescence was minimal. However, BHK-21 cells were found more sensitive than Vero cells for the detection of rubella-specific IgM antibodies. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-041
更新日期:1975-03-01 00:00:00
abstract::Five strains of Haemophilus pleuropneumoniae, out of eight strains tested, produced extracellular haemolysin(s) when grown in liquid culture in the presence, but not in the absence, of RNA. The haemolysin produced by the neotype strain was unstable, heat labile, and sensitive to degradation by pronase, trypsin, and ch...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-085
更新日期:1985-05-01 00:00:00
abstract::Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-239
更新日期:1975-10-01 00:00:00
abstract::Bacterial communities that can degrade surfactants have been selected from coastal seawaters contaminated by urban sewages. Only the linear fraction of commercial anionic surfactants was quickly degraded, and a residual fraction representing 10% of the initial concentration always remained. The highest concentrations ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::Pyrenocine A, a phytotoxin produced by Pyrenochaeta terrestris (Hansen) Gorenz, Walker and Larson, possesses general antibiotic activity against plants, fungi, and bacteria. Effective doses for 50% inhibition (ED50s) are 4 micrograms/mL for onion seedling elongation; 14, 20, 20, and 25 micrograms/mL for the germinatio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-055
更新日期:1987-04-01 00:00:00
abstract::Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-119
更新日期:2005-02-01 00:00:00
abstract::Strict anaerobic cultures of Mucor hiemalis lead to major modifications of the quantitative and qualitative composition of sterols. Sterols of which the lateral chain contains an ethyl or ethylidene group in C24 are for the first time brought to evidence in a Mucorales. Isolation in the same conditions of E-ethylidene...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-095
更新日期:1983-05-01 00:00:00
abstract::Leptospira interrogans serotype pomona was isolated from the kidneys of a normal striped skunk (Mephitis mephitis hudsonicus) collected near Kindersley, Saskatchewan. Although there were no gross lesions, small foci of chronic interstitial nephritis were observed microscopically. This is the first reported isolation o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-237
更新日期:1977-12-01 00:00:00
abstract::The types of bacteria and fungi present in the air of a municipal solid waste recovery system have been characterized and the population densities estimated. Conventional methods were successful in enumerating coagulase-positive staphylococci, Klebsiella spp., gram-negative bacteria, fungi, and common indicator organi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-038
更新日期:1985-03-01 00:00:00
abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0399
更新日期:2014-10-01 00:00:00
abstract::Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-099
更新日期:1991-08-01 00:00:00
abstract::Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to rec...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-056
更新日期:2008-08-01 00:00:00
abstract::The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-032
更新日期:1983-02-01 00:00:00
abstract::A total of 230 strains of metal-tolerant bacteria from water and sediment samples collected in Chesapeake Bay were isolated on a medium containing cobalt, lead, mercury, or molybdenum. In addition, a set of 71 cultures were simultaneously isolated on glucose tryptone yeast extract agar medium without metals. Twenty-th...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-212
更新日期:1977-10-01 00:00:00
abstract::A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-027
更新日期:1993-02-01 00:00:00
abstract::Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-173
更新日期:1987-11-01 00:00:00
abstract::Campylobacter spp. are a substantial cause of gastroenteritis worldwide. Human infection can result from ingestion of contaminated food or water from a variety of sources, including the consumption of fresh produce that is contaminated with the pathogen via the use of contaminated irrigation water. Using molecular met...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0710
更新日期:2016-05-01 00:00:00
abstract::Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have d...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-132
更新日期:1995-10-01 00:00:00
abstract::Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-068
更新日期:2007-08-01 00:00:00
abstract::Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-016
更新日期:1993-01-01 00:00:00
abstract::The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-112
更新日期:2004-02-01 00:00:00
abstract::A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-222
更新日期:1979-12-01 00:00:00
abstract::Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m95-091
更新日期:1995-08-01 00:00:00