Amino acid uptake systems in Bacteroides ruminicola.

Abstract:

:Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline were taken up and incorporated into cold trichloroacetic acid precipitable material. Different patterns of incorporation and different responses to 2,4-dinitrophenol and potassium ferricyanide indicated multiple uptake systems were involved. Kinetic inhibition patterns suggested six distinct systems were present for amino acid uptake, with specificities related to the chemical structures of the amino acids. Thus, the failure of free amino acids to act as sole nitrogen sources for growth of B. ruminicola is not due to the absence of transport systems for these compounds.

journal_name

Can J Microbiol

authors

Stevenson RM

doi

10.1139/m79-180

subject

Has Abstract

pub_date

1979-10-01 00:00:00

pages

1161-8

issue

10

eissn

0008-4166

issn

1480-3275

journal_volume

25

pub_type

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