Chemical and serological properties of a cyanogen bromide peptide of southern bean mosaic virus protein.

Abstract:

:Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to that of peptides released from EDTA-swollen virus particles by limited tryptic digestion. The composition and N-termini of the tryptic peptides indicated CB-1 was from the N-terminus of SBMV protein and contained 48 amino acid residues. The CB-1 peptide moved rapidly to the cathode in polyacrylamide gel electrophoresis at pH 3.9 and contained nine arginine residues, three lysine residues, and no acidic amino acid residues. It was shown to interact with purified viral RNA, sodium dextran sulfate, and calf thymus DNA. Antiserum to sodium dodecyl sulfate (SDS)-dissociated virus gave a reaction of partial identity between the CB-1 peptide and the SDS-dissociated virus in SDS gel diffusion tests. The CB-1 peptide did not react with antiserum to SDS-dissociated, trypsin-treated virus. Gel diffusion tests conducted in saline agar gels between trypsin-treated virus and SBMV, with SBMV antiserum, did not show differences in their serological properties. Antiserum to the CB-1 peptide conjugated to tomato bushy shunt virus reacted with SBMV but SBMV antiserum did not react with CB-1 or the CB-1-tomato bushy shunt virus conjugate.

journal_name

Can J Microbiol

authors

Tremaine JH,Ronald WP,Kelly EM

doi

10.1139/m80-241

subject

Has Abstract

pub_date

1980-12-01 00:00:00

pages

1450-9

issue

12

eissn

0008-4166

issn

1480-3275

journal_volume

26

pub_type

杂志文章
  • Generation and characterization of the antibacterial activity of a novel hybrid antimicrobial peptide comprising functional domains from different insect cecropins.

    abstract::The search for new antimicrobial compounds involves finding novel sources of chemotherapeutic compounds or manipulating and combining structures from existing molecules. Small antimicrobial peptides (AMPs) are components of innate immune defenses characterized in greatest detail in insect-derived AMPs. We have generat...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-001

    authors: Plunkett RM,Murray SI,Lowenberger CA

    更新日期:2009-05-01 00:00:00

  • Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution.

    abstract::Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and ot...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-44-11-1066

    authors: Bahirathan M,Puente L,Seyfried P

    更新日期:1998-11-01 00:00:00

  • Brucellosis in reindeer, Rangifer tarandus L., inoculated experimentally with Brucella suis, type 4.

    abstract::An investigation of brucellosis caused by Brucella suis, type 4, in reindeer, Rangifer tarandus L., and other ungulates inoculated experimentally with virulent isolates was undertaken to observe the course of infection, follow titres of serum agglutins, and determine the extent to which intraspecific and interspecific...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-024

    authors: Rausch RL,Huntley BE

    更新日期:1978-02-01 00:00:00

  • Biodegradation of carbazole by Ralstonia sp. RJGII.123 isolated from a hydrocarbon contaminated soil.

    abstract::The use of microorganisms for bioremediation of contaminated soils may be enhanced with an understanding of the pathways involved in their degradation of hazardous compounds. Ralstonia sp. strain RJGII.123 was isolated from soil located at a former coal gasification plant, based on its ability to mineralize carbazole,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w99-131

    authors: Schneider J,Grosser RJ,Jayasimhulu K,Xue W,Kinkle B,Warshawsky D

    更新日期:2000-03-01 00:00:00

  • A N2-fixing endophytic Burkholderia sp. associated with maize plants cultivated in Mexico.

    abstract::In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied. Under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-023

    authors: Estrada P,Mavingui P,Cournoyer B,Fontaine F,Balandreau J,Caballero-Mellado J

    更新日期:2002-04-01 00:00:00

  • Detection and characterization of bacteria from the potato rhizosphere degrading N-acyl-homoserine lactone.

    abstract::Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w06-062

    authors: Jafra S,Przysowa J,Czajkowski R,Michta A,Garbeva P,van der Wolf JM

    更新日期:2006-10-01 00:00:00

  • The biodegradation of methoxychlor by Klebsiella pneumoniae.

    abstract::Klebsiella pneumoniae was found to effect the reductive dechlorination of methoxychlor (2,2-bis(p-methoxyphenyl)-1, 1, 1-trichloroethane) and its dihydroxy derivative (2, 2-bis (p-hydroxyphenyl)-1, 1, 1-trichloroethane) to dichloro derivatives 2, 2-bis (p-methoxyphenyl)-1, 1-dichloroethane and 2, 2-bis (p-hydroxypheny...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-023

    authors: Baarschers WH,Bharath AI,Elvish J,Davies M

    更新日期:1982-02-01 00:00:00

  • Immunosuppression, nonspecific B-cell activation, and mitogenic activity associated with a high molecular weight component from Listeria monocytogenes.

    abstract::A high molecular component of a saline extract derived from Listeria monocytogenes contained amino acids, carbohydrates, and phosphorus. The same fraction was capable of promoting both the in vitro mitogenic and adjuvant activities and the in vivo immunosuppressive activity displayed by the crude extract. The material...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-204

    authors: Otokunefor TV,Galsworthy SB

    更新日期:1982-12-01 00:00:00

  • Relationship between clinical site of isolation and ability to form biofilms in vitro in nontypeable Haemophilus influenzae.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0763

    authors: Obaid NA,Jacobson GA,Tristram S

    更新日期:2015-03-01 00:00:00

  • Amino acid uptake systems in Bacteroides ruminicola.

    abstract::Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-180

    authors: Stevenson RM

    更新日期:1979-10-01 00:00:00

  • Antimicrobial susceptibilities and distribution of sequence types of Neisseria gonorrhoeae isolates in Canada: 2010.

    abstract::The monitoring of antimicrobial susceptibilities in Neisseria gonorrhoeae isolates and characterization of N. gonorrhoeae multiantigen sequence types (NG-MAST, ST) provide important surveillance data as resistance rates continue to rise. A total of 2970 N. gonorrhoeae isolates were collected by Canadian provincial pub...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0357

    authors: Martin I,Sawatzky P,Liu G,Allen V,Lefebvre B,Hoang L,Lovgren M,Haldane D,Caeseele PV,Horsman G,Garceau R,Ratnam S,Wong T,Gilmour M

    更新日期:2013-10-01 00:00:00

  • Sequencing of porA from clinical isolates of Neisseria meningitidis defines a subtyping scheme and its genetic regulation.

    abstract::Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Arhin FF,Moreau F,Coulton JW,Mills EL

    更新日期:1998-01-01 00:00:00

  • Disruption of the 2-methylcitric acid cycle and evaluation of poly-3-hydroxybutyrate-co-3-hydroxyvalerate biosynthesis suggest alternate catabolic pathways of propionate in Burkholderia sacchari.

    abstract::The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w09-018

    authors: Pereira EM,Silva-Queiroz SR,Cabrera Gomez JG,Silva LF

    更新日期:2009-06-01 00:00:00

  • Binding of mutagenic pyrolyzates to fractions of intestinal bacterial cells.

    abstract::The binding of mutagenic pyrolyzates to cell fractions from some gram-negative intestinal bacteria and to thermally treated bacterial cells was investigated. 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) were effectively bound by several of the bacterial ce...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-101

    authors: Zhang XB,Ohta Y

    更新日期:1992-07-01 00:00:00

  • Restriction analysis of actinomycetes chromosomal DNA.

    abstract::Actinomycetes DNAs were digested with restriction enzymes to study the presence of methylated bases. Analysis showed that the enterobacterial Dam and Dcm systems are absent. Methylation at the internal cytosine in CCGG sequences, typical of eukaryotes, was also absent. We also tested 18 restriction endonucleases recog...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-031

    authors: Novella IS,Marín I,Sánchez J

    更新日期:1996-02-01 00:00:00

  • Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants.

    abstract::Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-015

    authors: Pfeifer F,Blaseio U,Horne M

    更新日期:1989-01-01 00:00:00

  • An efficient system for markerless gene replacement applicable in a wide variety of enterobacterial species.

    abstract::We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w06-102

    authors: White AP,Allen-Vercoe E,Jones BW,DeVinney R,Kay WW,Surette MG

    更新日期:2007-01-01 00:00:00

  • Cell surface properties of two differently virulent strains of Acinetobacter baumannii isolated from a patient.

    abstract::The aim of this study was to unravel, by focusing on cell surface properties, the underlying virulence factors contributing to the difference in the pathogenicity observed in two Acinetobacter baumannii strains isolated from the same patient. The two strains were phenotypically different: (i) a mucoid strain (AB-M),...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-131

    authors: Kempf M,Eveillard M,Deshayes C,Ghamrawi S,Lefrançois C,Georgeault S,Bastiat G,Seifert H,Joly-Guillou ML

    更新日期:2012-03-01 00:00:00

  • Comparative studies of the mosquito-larval toxin of Bacillus sphaericus SSII-1 and 1593.

    abstract::Two strains of Bacillus sphaericus. SSII-1 and 1593, were bioassayed for toxic activity against second-instar larvae of the mosquito Culex pipiens quinquefasciatus. It was found that strain 1593 developed a level of toxicity 3000 times that of strain SSII-1. Although the toxic activity of B. sphaericus SSII-1 was rela...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-193

    authors: Myers P,Yousten AA,Davidson EW

    更新日期:1979-11-01 00:00:00

  • The nonrandom binding distribution of Streptococcus pneumoniae to type II pneumocytes in culture is dependent on the relative distribution of cells among the phases of the cell cycle.

    abstract::The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Bérubé LR,Jouishomme H,Jarrell HC

    更新日期:1998-05-01 00:00:00

  • Mapping of genes encoding glycoside hydrolases on the chromosome of Cellulomonas fimi.

    abstract::Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Stoll D

    更新日期:2001-12-01 00:00:00

  • Immunovirological studies on human respiratory syncytial virus structural proteins.

    abstract::Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-004

    authors: Trudel M,Nadon F,Séguin C,Ghoubril S,Payment P,Trépanier P

    更新日期:1986-01-01 00:00:00

  • Genomic comparison of facultatively anaerobic and obligatory aerobic Caldibacillus debilis strains GB1 and Tf helps explain physiological differences.

    abstract::Caldibacillus debilis strains GB1 and Tf display distinct phenotypes. Caldibacillus debilis GB1 is capable of anaerobic growth and can synthesize ethanol while C. debilis Tf cannot. Comparison of the GB1 and Tf genome sequences revealed that the genomes were highly similar in gene content and showed a high level of sy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0464

    authors: Wushke S,Froese A,Fristensky B,Zhang XL,Spicer V,Krokhin OV,Levin DB,Sparling R

    更新日期:2019-06-01 00:00:00

  • Rapid comparison of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris using polymerase chain reaction amplification.

    abstract::Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequenc...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m93-059

    authors: Kwoh DY,Vedvick TS,McCue AF,Gevertz D

    更新日期:1993-04-01 00:00:00

  • Presence of a new cytochrome b - like pigment with a peak at 567 nm in various aerobic bacteria.

    abstract::Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-177

    authors: Jacobs NJ,O'Hara J,Gray CT

    更新日期:1983-09-01 00:00:00

  • Prevalence of Giardia and Cryptosporidium and characterization of Cryptosporidium spp. isolated from wildlife, human, and agricultural sources in the North Saskatchewan River Basin in Alberta, Canada.

    abstract::The environmental distribution of Giardia spp. and Cryptosporidium spp. is dependent upon human, agricultural, and wildlife sources. The significance of each source with regard to the presence of parasites in the environment is unknown. This 2-year study examined parasite prevalence in human sewage influent, wildlife,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-047

    authors: Heitman TL,Frederick LM,Viste JR,Guselle NJ,Morgan UM,Thompson RC,Olson ME

    更新日期:2002-06-01 00:00:00

  • Stress-survival responses of a carbon-starved p-nitrophenol-mineralizing Moraxella strain in river water.

    abstract::The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-131

    authors: Moore M,Trevors J,Lee H,Leung KT

    更新日期:2005-03-01 00:00:00

  • Improving the culturability of freshwater bacteria using FW70, a low-nutrient solid medium amended with sodium pyruvate.

    abstract::Bacterial culture based on the use of plate media is an effective method for investigating bacterial populations in the environment. To improve the culturability of bacteria from freshwater lakes, we developed a new medium, FW70, which contains sodium pyruvate, casamino acids, and lake water and is solidified using ge...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-019

    authors: Imazaki I,Kobori Y

    更新日期:2010-04-01 00:00:00

  • Morphologic and functional effects of Clostridium difficile enterotoxin in tissue culture.

    abstract::The effects of the Clostridium difficile toxin were examined in HeLa and mouse adrenal tumor (MAT) cells. Cytotoxicity was evaluated by vital dye exclusion and 51Cr release. In both HeLa and MAT cells, C. difficile toxin caused rounding of virtually 100% of cells. This rounding was distinguishable from rounding produc...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-009

    authors: Gurwith MJ,Langston C,Dunsmore B

    更新日期:1982-01-01 00:00:00

  • [The effect of pyridoxal phosphate on the tryptophanases of five species of Enterobacteriaceae].

    abstract::Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris et P. morganii tryptophanases (TPases) were studied for the spectral forms of the enzyme. The pH effect on the absorption spectrum and on the enzyme specific activity revealed that the coli group TPases are identical with but differ from P...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Simard C,Mardini A,Bordeleau LM

    更新日期:1975-06-01 00:00:00