Abstract:
:Urease activity was measured using whole cells of both long (swarming) and short (nonswarming) populations of Proteus mirabilis from casein hydrolysate agar (CHA) and broth (CHB) cultures, and from brain heart infusion broth (BHIB) cultures. Urease is a constitutive enzyme for both long and short cells, but its activity was tremendously increased when urea was incorporated into the media. Urease production was also affected by culture age and media used. Before exponential phase, urease activity was very low, and it increased to its highest point after about 4 h in BHIB and 8 h in both CHA and CHB cultures at 37 degrees C. Long cells had higher urease activity than did short cells when grown on CHA, and was also expressed by two different strains cultured in BHIB. Strain PM23, in BHIB, was able to form long cells (swarming cells) to a maximum proportion after about 4 h, but strain IM47 could not differentiate in any of the liquid media. The former had more urease when swarming differentiation was initiated. PM23 grew relatively faster than IM47 when the former began to differentiate, but this fast growth could not be observed when nutrient broth or minimal medium was used. These observations suggest that long or swarming cells are "faster growing" rather than "nongrowing bacteria".
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Jin T,Murray RGdoi
10.1139/m87-051subject
Has Abstractpub_date
1987-04-01 00:00:00pages
300-3issue
4eissn
0008-4166issn
1480-3275journal_volume
33pub_type
杂志文章abstract::Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-038
更新日期:2004-08-01 00:00:00
abstract::Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/m95-091
更新日期:1995-08-01 00:00:00
abstract::Heat activation (70 degrees C for 20 min) resulted in alteration in structural proteins and enzymes found in Bacillus cereus spore coats. The three notable changes were increased glycosylation of coat proteins, alteration in polypeptide pattern on sodium dodecyl sulfate - polyacrylamide gels, and an increase in free S...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-062
更新日期:1981-04-01 00:00:00
abstract::A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-162
更新日期:1997-12-01 00:00:00
abstract::Histological examination of oesophagus and stomach specimens from 28 guinea pigs (Cavia porcellus) revealed microbes associated with the epithelial surface of these organs. Layers of Gram-positive cocci were associated with oesophageal tissue in all the animals. Yeasts (Torulop-sid pintolopesii) were associated with s...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-182
更新日期:1978-09-01 00:00:00
abstract::Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-012
更新日期:2006-06-01 00:00:00
abstract::Pleomorphic deterioration is a process where a fungal isolate loses the ability to produce conidia during repeated subculturing. We have previously isolated strains of the entomopathogenic fungus Metarhizium anisopliae that have irreversibly lost the ability to produce conidia and only produce mycelia when grown on ag...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-074
更新日期:2002-09-01 00:00:00
abstract::Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-073
更新日期:1997-06-01 00:00:00
abstract::Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-220
更新日期:1985-12-01 00:00:00
abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-070
更新日期:1996-05-01 00:00:00
abstract::The use of microorganisms for bioremediation of contaminated soils may be enhanced with an understanding of the pathways involved in their degradation of hazardous compounds. Ralstonia sp. strain RJGII.123 was isolated from soil located at a former coal gasification plant, based on its ability to mineralize carbazole,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w99-131
更新日期:2000-03-01 00:00:00
abstract::We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-008
更新日期:1992-01-01 00:00:00
abstract::Nonmotile and motility-altered mutants of Myxococcus xanthus have been obtained by the use of chemical mutagens, ultraviolet irradiation, and a procedure for selective spontaneous mutants. As judged by their behaviour on a variety of growth media, in both plate and slide culture, the mutants were divided into four gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-190
更新日期:1976-09-01 00:00:00
abstract::A defined medium (CDM) is described which supported growth and sporulation of type E strains of Clostridium botulinum, but not sporulation of other serotypes of C. botulinum or C. sporogenes. As compared to growth in complex medium, spore outgrowth was delayed and both the growth rate and the cell yield was reduced. H...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-076
更新日期:1979-04-01 00:00:00
abstract::A Tn551 insertional pleiotropic mutant defective in the production of several exoproteins was isolated from Staphylococcus aureus 196E and characterized. The pleiotropism of the mutant was due to a single insertion of the transposon as evidenced by Southern blot hybridization and by the transfer of its phenotype by tr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-107
更新日期:1994-08-01 00:00:00
abstract::Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and ot...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-44-11-1066
更新日期:1998-11-01 00:00:00
abstract::Streptococcus pneumoniae infection starts from the respiratory tract where interaction with host epithelial cells occurs. To gain more insights on pneumococcal pathogenesis, an oligonucleotide (oligo)-based microarray was used to investigate gene expression changes of one serotype 3 encapsulated pathogenic S. pneumoni...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w07-133
更新日期:2008-03-01 00:00:00
abstract::Nitrogen-fixing, field-obtained root nodules of the silky green alder were studied by transmission electron microscopy. The nodule endophyte exhibited a prokaryotic cytology and was present in two forms: the hypha(0.3-1.0mum), which was branched and septate, and the vesicle (3-5mum), which was also septate and develo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-157
更新日期:1975-07-01 00:00:00
abstract::To facilitate the recognition of plant viruses with similar characteristics a dendrogram of characterized viruses was constructed. The sequence of criteria included: type of nucleic acid; single or double stranded; presence or absence of lipid envelope; helical or nonhelical symmetry; and divided or single genome. Non...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-197
更新日期:1977-09-01 00:00:00
abstract::Filamentous growth of Candida albicans occurs in response to a variety of environmental signals. The C. albicans gene orf19.1944 and its allele orf19.9499 are identical and are predicted to encode an 823-residue, 7-transmembrane-domain protein that has all the expected features of a G-protein-coupled receptor. The pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-095
更新日期:2004-12-01 00:00:00
abstract::Environmental factors influencing the occurrence and community structure of soil yeasts in forests are not well studied. There are few studies dedicated to Southern Hemisphere soil yeasts populations and even fewer focused on temperate forests influenced by volcanic activity. The present work aimed to study the ecolog...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0897
更新日期:2014-06-01 00:00:00
abstract::Unicellular green alga represents not only a convenient model for its biochemical and physiological studies but also a sensitive system to test the effects of various environmental factors. Algae cells of two strains, SA-3 strain (exsymbiotic from Paramecium bursaria) and Chlorella vulgaris c-27, were asynchronously c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-143
更新日期:2002-02-01 00:00:00
abstract::The introduction of the 7-valent pneumococcal vaccine (PCV7) in Canada was very effective in reducing invasive pneumococcal disease (IPD) in children; however, increases of non-PCV7 serotypes have subsequently offset some of these reductions. A 13-valent pneumococcal vaccine (PCV13) targeting additional serotypes was ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0614
更新日期:2013-12-01 00:00:00
abstract::The gene for a type IIo IgG-binding protein has previously been cloned and sequenced. The approximately 60,000 M(r) recombinant gene product binds all four human IgG subclasses and fibrinogen. Treatment of this recombinant protein with CNBr results in generation of a series of fragments. One fragment, an approximately...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-149
更新日期:1996-11-01 00:00:00
abstract::We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-126
更新日期:1992-08-01 00:00:00
abstract::Studies show that Paracoccus denitrificans can denitrify nitrogen sources under aerobic conditions. However, the lack of data on its genome sequence has restricted molecular studies and practical applications. In this study, the complete genome of P. denitrificans ATCC 19367 was sequenced and its nitrogen metabolism p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0037
更新日期:2019-07-01 00:00:00
abstract::Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-164
更新日期:1984-08-01 00:00:00
abstract::We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-059
更新日期:1988-03-01 00:00:00
abstract::Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-099
更新日期:2004-12-01 00:00:00