Abstract：
:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation augments host resistance against the lethal toxicity of 5-FU in tumor-bearing mice. Intravenous administration of a preparation of heat-killed Lactobacillus casei (LC 9018), a nonspecific immunostimulant, at a dose of 20 mg/kg to BALB/c mice augmented their resistance against the lethal toxicity of 5-FU if the preparation was injected into the mice 10-40 days before administration of 5-FU. Injection of LC 9018 into BALB/c mice bearing Meth A fibrosarcoma also enhanced their resistance against the lethality of 5-FU. Systemic infection with E. coli was induced in all of the 5-FU-treated tumor-bearing mice 10 days or more after administration of the drug at a lethal dose of 500 mg/kg, and it was accompanied by an overgrowth of the bacteria in the intestine. Treatment of tumor-bearing mice with LC 9018 resulted in decreased rates of occurrence of systemic infection with E. coli and inhibition of overgrowth of the bacteria in the intestine after administration of 5-FU. A single administration of either LC 9018 or 5-FU significantly inhibited the growth of Meth A cells in vivo, and a combined antitumor effect was shown in the mice treated with both 5-FU and LC 9018.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Nomoto K,Yokokura T,Nomoto Kdoi
10.1139/m92-126subject
Has Abstractpub_date
1992-08-01 00:00:00pages
774-8issue
8eissn
0008-4166issn
1480-3275journal_volume
38pub_type
杂志文章abstract：:Cells of five Bacteroides species were examined following treatment with homologous antisera and staining with ruthenium red. They were enveloped by glycocalyces and these extensive fibrous exopolysaccharide matrices were fully retained as an integral "capsule" by some cells, while other cells showed "capsule" as well...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-124
更新日期：1984-06-01 00:00:00
abstract：:Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
abstract：:Penicillium chrysogenum F1 is very efficient in bioleaching heavy metals from the soil and is used for that purpose. We found that F1 can extract 19.8 mg Cd, Cu, Pb, and Zn from 2.5 g soil; the total heavy metals' bioleaching ratio was 60.4%. In this study, the bioleaching mechanism was investigated by means of metabo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0624
更新日期：2019-09-01 00:00:00
abstract：:Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-132
更新日期：1995-10-01 00:00:00
abstract：:Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-013
更新日期：2004-04-01 00:00:00
abstract：:The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0814
更新日期：2016-06-01 00:00:00
abstract：:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-095
更新日期：1986-06-01 00:00:00
abstract：:A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-155
更新日期：1977-08-01 00:00:00
abstract：:Two rod-shaped viruses of similar length occurring in hops were isolated from a mixed infection. One (hop mosaic virus) infected Nicotiana clevelandii, produced mosaic symptoms in mosaic-sensitive hop cultivars, and did not infect Chenopodium murale. The other (hop latent virus) infected C. murale but not N. cleveland...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-168
更新日期：1976-08-01 00:00:00
abstract：:Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-046
更新日期：1979-03-01 00:00:00
abstract：:The ruminal microbiota plays major roles in feed digestion. The composition and fermentation of the bacterial communities in 3 important ruminant species have been studied previously. Here, we extended this research to the effect of concentrate-to-forage ratios on ruminal bacterial communities in Tarim red deer (Cervu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0046
更新日期：2018-07-01 00:00:00
abstract：:The goal of this study was to evaluate the antibiofilm and antimicrobial activities of Bacoside A, a formulation of phytochemicals from Bacopa monnieri, against Staphylococcus aureus and Pseudomonas aeruginosa, which are known to form biofilms as one of their virulence traits. The antimicrobial effects of Bacoside A w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0365
更新日期：2017-02-01 00:00:00
abstract：:The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-012
更新日期：1975-01-01 00:00:00
abstract：:Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-065
更新日期：1976-03-01 00:00:00
abstract：:Acid (AcP) and alkaline phosphatase (AlP) were localized by physicobiochemical techniques. Greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted Capnocytophaga species released only 9-28% and 11-43% of the cellular pho...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-212
更新日期：1983-10-01 00:00:00
abstract：:Escherichia coli B and E. aurescens, Shigella alkalescens, and Proteus vulgaris et P. morganii tryptophanases (TPases) were studied for the spectral forms of the enzyme. The pH effect on the absorption spectrum and on the enzyme specific activity revealed that the coli group TPases are identical with but differ from P...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
abstract：:Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-064
更新日期：1989-03-01 00:00:00
abstract：:During shadowing, a "cap" of metal develops on small particles. This cap increases apparent particle with (measured normal to the shadowing direction) by an extent which cannot be predetermined. The extent of this increase in particle size (here defined as the "cap," X) is estimated in the present method by using oppo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-114
更新日期：1985-07-01 00:00:00
abstract：:Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-068
更新日期：2007-08-01 00:00:00
abstract：:There are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. Fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. Through surveys of four bathing beaches on Lake Ontario, it w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-159
更新日期：1979-09-01 00:00:00
abstract：:The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cyto...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0606
更新日期：2013-06-01 00:00:00
abstract：:Arsenic hyperaccumulator fern Pteris vittata L. produces large amounts of root exudates that are hypothesized to solubilize arsenic and maintain a unique rhizosphere microbial community. Total heterotrophic counts on rich or defined media supplemented with up to 400 mmol/L of arsenate showed a diverse arsenate-resista...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-005
更新日期：2010-03-01 00:00:00
abstract：:The rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries) are important ruminant species for animal husbandry in the Tarim Basin. However, the microbiota and rum...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0596
更新日期：2017-05-01 00:00:00
abstract：:Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0206
更新日期：2014-08-01 00:00:00
abstract：:Nitrogenase in Azotobacter chroococcum whole cells was inhibited by enzymically generated superoxide anion (O2-), hydrogen peroxide, and ethyl hydrogen peroxide. The degree of inhibition produced by O2- was related to the quantity of oxygen supplied to the organisms in continuous cultures. O2- also inhibited oxygen up...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-228
更新日期：1977-11-01 00:00:00
abstract：:Fluorescent antibody was prepared against a temperate-soil isolate of Beijerinckia obtained from a rhizosphere of rice growing in Camargue (France). The antibody did not cross-react with any of 6 species of Azotobacter, 4 species of Beijerinckia, or 44 unidentified soil bacteria isolated from a spectrum of rhizosphere...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-023
更新日期：1977-02-01 00:00:00
abstract：:Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-153
更新日期：1978-08-01 00:00:00
abstract：:Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-063
更新日期：2012-07-01 00:00:00
abstract：:Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-099
更新日期：2004-12-01 00:00:00
abstract：:Isoquinoline is a nitrogen heterocyclic compound that is associated with coal- and oil-derived wastes. Four strains of bacteria able to degrade isoquinoline in pure culture were isolated from sites known to be contaminated with oil. Isoquinoline was used as the sole source of carbon and nitrogen by these isolates. Iso...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-089
更新日期：1994-07-01 00:00:00
