Morphological stabilization of the glycocalyces of 23 strains of five Bacteroides species using specific antisera.

Abstract:

:Cells of five Bacteroides species were examined following treatment with homologous antisera and staining with ruthenium red. They were enveloped by glycocalyces and these extensive fibrous exopolysaccharide matrices were fully retained as an integral "capsule" by some cells, while other cells showed "capsule" as well as detached glycocalyx components forming an intercellular "slime.". These extensive glycocalyces collapsed during dehydration for electron microscopy and formed electron-dense accretions on cell surfaces and electron-dense reticula in intercellular spaces when the cells were treated with heterologous antiserum or when antibody stabilization was omitted. The glycocalyces of all strains, both stabilized and unstabilized, were observed outside the outer membranes of cell walls that showed the "classic" gram-negative structural organization. Appropriate modifications of the indirect fluorescent antibody test demonstrated an integral "capsule" on all strains examined; detached glycocalyx and varying amounts of slime were demonstrated after stabilization with homologous, but not heterologous, antiserum.

journal_name

Can J Microbiol

authors

Lambe DW Jr,Mayberry-Carson KJ,Ferguson KP

doi

10.1139/m84-124

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

809-19

issue

6

eissn

0008-4166

issn

1480-3275

journal_volume

30

pub_type

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