Abstract:
:Cells of five Bacteroides species were examined following treatment with homologous antisera and staining with ruthenium red. They were enveloped by glycocalyces and these extensive fibrous exopolysaccharide matrices were fully retained as an integral "capsule" by some cells, while other cells showed "capsule" as well as detached glycocalyx components forming an intercellular "slime.". These extensive glycocalyces collapsed during dehydration for electron microscopy and formed electron-dense accretions on cell surfaces and electron-dense reticula in intercellular spaces when the cells were treated with heterologous antiserum or when antibody stabilization was omitted. The glycocalyces of all strains, both stabilized and unstabilized, were observed outside the outer membranes of cell walls that showed the "classic" gram-negative structural organization. Appropriate modifications of the indirect fluorescent antibody test demonstrated an integral "capsule" on all strains examined; detached glycocalyx and varying amounts of slime were demonstrated after stabilization with homologous, but not heterologous, antiserum.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Lambe DW Jr,Mayberry-Carson KJ,Ferguson KPdoi
10.1139/m84-124subject
Has Abstractpub_date
1984-06-01 00:00:00pages
809-19issue
6eissn
0008-4166issn
1480-3275journal_volume
30pub_type
杂志文章abstract::Vesicular-arbuscular mycorrhizae of yellow poplar (Liriodendron tulipifera L.) were examined by scanning electron microscopy. The morphology and spatial distribution of endophytic structures are illustrated. The use of stereoscopic techniques in conjunction with removal of host cytoplasm permits detection of subtle ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-146
更新日期:1975-07-01 00:00:00
abstract::The study of the relationship between MVL 51 virus, of the Gourlay group L 1, and the Mycoplasma cells of A. laidlawii during the first 35 min of infection leads to the conclusion that a "carrier state" has been established, free virus as well as infected and noninfected cells being present at the same time in the cul...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::The purpose of this study was to evaluate experimentally the immunogenicity in rabbits of rubella subunits adsorbed to the adjuvant Quil A. The adsorbed viral proteins form structurally defined ImmunoStimulating COMplexes (ISCOMs). Rubella ISCOMs were tested for their capacity to induce neutralizing and hemagglutinati...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-238
更新日期:1988-12-01 00:00:00
abstract::Shiga-toxin-producing Escherichia coli (STEC) represent a major concern for waterborne disease outbreaks associated with consumption of contaminated groundwater. Over 4 million people rely on private groundwater systems as their primary drinking water source in Canada; many of these systems do not meet current standar...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/cjm-2019-0329
更新日期:2020-05-01 00:00:00
abstract::A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-162
更新日期:1997-12-01 00:00:00
abstract::A strain of Pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. A CoA-mediated beta-oxidation pathway was induced for the metabolism of the compound. The organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-198
更新日期:1982-12-01 00:00:00
abstract::Epiphytic tracer bacteris (a Pseudomonas sp. and an Arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. Of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. Seed from two of these pods also carried tracers. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-064
更新日期:1976-03-01 00:00:00
abstract::Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-020
更新日期:1988-02-01 00:00:00
abstract::The aim of the present study was to examine soil samples from various vegetation zones in terms of physicochemical properties, microbial communities, and isolation and identification (by polymerase chain reaction and transmission electron microscopy) of bacteria producing poly-β-hydroxybutyrates (PHBs). Soil samples w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-037
更新日期:2012-05-01 00:00:00
abstract::The effect of fructans on the cultivable faecal community of Bio Breeding rats fed diets containing 5% (m/v) food-grade fructooligosaccharide (FOS) was investigated. Culturing of faecal material using chicory inulin as the sole carbohydrate source revealed the presence of a greater diversity of inulin-utilizing bacter...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-053
更新日期:2006-10-01 00:00:00
abstract::The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0770
更新日期:2016-07-01 00:00:00
abstract::Nonlinear survivor curves were obtained when spores of Bacillus cereus were heated in physiological saline solution. Curvilinear survivor curves did not appear to be caused by experimental artifacts but by the heterogeneity of spore population with regard to heat resistance. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-217
更新日期:1975-10-01 00:00:00
abstract::We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-126
更新日期:1992-08-01 00:00:00
abstract::In a previous study it was shown that the number of wood-inhabiting bacteria was drastically reduced after colonization of beech (Fagus sylvatica) wood blocks by the white-rot fungus Hypholoma fasciculare, or sulfur tuft (Folman et al. 2008). Here we report on the mechanisms of this fungal-induced antibacterial activi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-023
更新日期:2010-05-01 00:00:00
abstract::Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-083
更新日期:2005-11-01 00:00:00
abstract::Mutations in the DNA polymerase locus of phage, bacteria, and eukaryotic may change the mutation rates at other loci of the genome. We used resistance to phosphonoacetate to select mutants of herpes simplex virus with mutated DNA polymerase and then determined the reversion frequency of viral thymidine kinase mutation...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-084
更新日期:1984-05-01 00:00:00
abstract::Long-term survival, dispersal, and germination of Bacillus thuringiensis var. kurstaki DMU67R has been investigated in a field trial. An experimental cabbage plot was sprayed with DMU67R in 1993 and allowed to lie fallow since. The investigations reported here were carried out from 1997 to 2000 in this plot. High pers...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-009
更新日期:2002-03-01 00:00:00
abstract::Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-195
更新日期:1977-09-01 00:00:00
abstract::The addition of various concentrations of monensin (1,5, and 10 micrograms/mL) to the culture medium inhibits the fungal growth and perturbs exopolysaccharides secretion, provoking a decrease of production in Botrytis cinerea and an increase in Sclerotium rolfsii. The ionophore induces also modifications in both polym...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-124
更新日期:1996-09-01 00:00:00
abstract::Mixtures of anionic HgCl3-/HgCl4(2)-complexes were less toxic to terrestrial bacteria (Erwinia herbicola, Agrobacterium tumefaciens), to marine bacteria (Acinetobacter sp., Aeromonas sp.), and to bacteriophages (phi 11 M 15 of Staphylococcus aureus and P1 of Escherichia coli) than were equivalent concentrations of Hg ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-197
更新日期:1979-11-01 00:00:00
abstract::The use of microbial fertilizers can help to avoid the harmful effects of traditional agricultural fertilizers and pesticides; however, there are many constraints on the practical application of such fertilizers. In this study, microbial biochar formulations (MBFs) were obtained by loading biochar, created from agricu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0333
更新日期:2019-05-01 00:00:00
abstract::A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-151
更新日期:1977-08-01 00:00:00
abstract::Leptospira interrogans serotype pomona was isolated from the kidneys of a normal striped skunk (Mephitis mephitis hudsonicus) collected near Kindersley, Saskatchewan. Although there were no gross lesions, small foci of chronic interstitial nephritis were observed microscopically. This is the first reported isolation o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-237
更新日期:1977-12-01 00:00:00
abstract::When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W08-035
更新日期:2008-06-01 00:00:00
abstract::A study has been undertaken to account for the presence of Thiobacillus acidophilus in iron-grown cultures of Thiobacillus ferrooxidans. Attempts to adapt T. acidophilus to ferrous iron were not successful but the facultative autotroph grew to a limited extent in the spent medium of T. ferrooxidans and was able to gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-178
更新日期:1980-09-01 00:00:00
abstract::Mycoplasma pneumoniae is an important causative agent of atypical pneumonia. This study was to determine the ability of a DNA expression vector, which encodes the carboxy terminal region of the M. pneumoniae P1 protein (P1C), to induce humoral and cellular immune responses and to protect against M. pneumoniae infecti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-041
更新日期:2012-05-01 00:00:00
abstract::Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-015
更新日期:1989-01-01 00:00:00
abstract::Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-177
更新日期:1983-09-01 00:00:00
abstract::Bacteria are known to rapidly produce new phenotypes, but it is unclear how phenotype "plasticity" relates to studies on the population ecology of bacteria in complex environments. We characterised a collection of 14 spontaneous phenotype variants, derived from in vitro and in vivo cultures (wheat roots) of Pseudomona...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w99-006
更新日期:1999-04-01 00:00:00
abstract::The antimicrobial activity of Ag, Cu, and layered Ag-Cu surface films, sputter-coated onto several types of catheter material, against clinical isolates of Staphylococcus epidermidis and Staphylococcus aureus was evaluated. When 20 microL of a suspension of S. epidermidis or S. aureus (2.68 x 10(6) colony-forming unit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-134
更新日期:1993-09-01 00:00:00