Efficient production of Aschersonia placenta protoplasts for transformation using optimization algorithms.

Abstract:

:The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based on the response surface method (RSM). Critical parameters for protoplast production were modelled by using a Box-Behnken design (BBD) involving 3 levels of 3 variables that was subsequently tested to verify its ability to predict protoplast production (R(2) = 0.9465). The optimized conditions resulted in the highest yield of protoplasts ((4.41 ± 0.02) × 10(7) cells/mL of culture, mean ± SE) when fungal cells were treated with 26.1 mg/mL of lywallzyme for 4 h of digestion, and subsequently allowed to recover for 64.6 h in 0.7 mol/L NaCl-Tris buffer. The latter was used as an osmotic stabilizer. The yield of protoplasts was approximately 10-fold higher than that of the nonoptimized conditions. Generated protoplasts were transformed with vector PbarGPE containing the bar gene as the selection marker. Transformation efficiency was 300 colonies/(μg DNA·10(7) protoplasts), and integration of the vector DNA was confirmed by PCR. The results show that rational design strategies (RSM and BBD methods) are useful to increase the production of fungal protoplasts for a variety of downstream applications.

journal_name

Can J Microbiol

authors

Wei X,Song X,Dong D,Keyhani NO,Yao L,Zang X,Dong L,Gu Z,Fu D,Liu X,Qiu J,Guan X

doi

10.1139/cjm-2015-0770

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

579-87

issue

7

eissn

0008-4166

issn

1480-3275

journal_volume

62

pub_type

杂志文章
  • Persistence of naturally acquired cell-mediated immunity to rubella virus during the first trimester of pregnancy.

    abstract::We studied the persistence of naturally acquired cell-mediated immunity to rubella during early pregnancy. We compared lymphocyte transformation responses to phytohemagglutinin and rubella virus in 35 naturally immune women aged 17-37 years, in the first trimester of pregnancy, with 49 naturally immune age-matched non...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-257

    authors: Rossier E,Phipps PH,Weber JM

    更新日期:1983-12-01 00:00:00

  • The presence of Listeria spp. in raw milk in Ontario.

    abstract::Raw milk samples from bulk tanks in Ontario were analyzed for the presence of Listeria species. The overall incidence of Listeria species in raw milk was 12.4%. Listeria innocua was most frequently isolated and was found in 9.7% (43/445) of the raw milk samples, while L. monocytogenes and L. welshimeri were each found...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-020

    authors: Farber JM,Sanders GW,Malcolm SA

    更新日期:1988-02-01 00:00:00

  • Differentiation of Rhizobium japonicum strain derivatives by antibiotic sensitivity patterns, lectin binding, and utilization of biochemicals.

    abstract::Several strains of Rhizobium japonicum have been reported to consist of mixtures of stable derivativess having distinct colony morphologies and physiological characteristics. We isolated derivatives from strains of R. japonicum and systematically compared them with previously isolated derivatives with respect to the u...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-106

    authors: Meyer MC,Pueppke SG

    更新日期:1980-05-01 00:00:00

  • Degradation of [14C]photodieldrin by Trichoderma viride as affected by other insecticides.

    abstract::Various soil fungi were tested for their capacity to degrade the insecticide [14C]photodieldrin. Of nine species investigated, Trichoderma viride was the only one which degraded the insecticide to an appreciable extent into water-soluble, non-insecticidal compounds within 4-5 weeks. These products amounted to 32-41% o...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-198

    authors: Tabet JC,Lichtenstein EP

    更新日期:1976-09-01 00:00:00

  • Soybean flower-to-seed movement of epiphytic bacteria.

    abstract::Epiphytic tracer bacteris (a Pseudomonas sp. and an Arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. Of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. Seed from two of these pods also carried tracers. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-064

    authors: Leben C

    更新日期:1976-03-01 00:00:00

  • Control of pyrimidine formation in Pseudomonas putida ATCC 17536.

    abstract::The regulation of de novo pyrimidine biosynthesis in Pseudomonas putida ATCC 17536 by pyrimidines was explored. The pathway enzyme activities were higher in glucose-grown cells than in succinate-grown cells, indicating catabolite repression by succinate. In P. putida cells grown on succinate as a carbon source, only a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-110

    authors: Santiago MF,West TP

    更新日期:2002-12-01 00:00:00

  • Characteristics of the rhizobia associated with Dalea spp. in the Ordway, Kellogg-Weaver Dunes, and Hayden prairies.

    abstract::Habitat fragmentation affects the biodiversity and function of aboveground organisms in natural ecosystems but has not been studied for effects on belowground species. In this paper, we consider the diversity of the rhizobia associated with the indigenous legume Dalea purpurea in 3 residual prairie areas in Minnesota ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-107

    authors: Tlusty B,van Berkum P,Graham PH

    更新日期:2005-01-01 00:00:00

  • Occurrence and identification of microorganisms in compacted clay-based buffer material designed for use in a nuclear fuel waste disposal vault.

    abstract::A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m97-162

    authors: Stroes-Gascoyne S,Pedersen K,Haveman SA,Dekeyser K,Arlinger J,Daumas S,Ekendahl S,Hallbeck L,Hamon CJ,Jahromi N,Delaney TL

    更新日期:1997-12-01 00:00:00

  • Bacteriophage T4 multiplication in a glucose-limited Escherichia coli biofilm.

    abstract::An Escherichia coli K-12 biofilm was grown at a dilution rate of 0.028 h(-1) for 48 h in a glucose-limited chemostat coupled to a modified Robbins' device to determine its susceptibility to infection by bacteriophage T4. Bacteriophage T4 at a multiplicity of infection (MOI) of 10 caused a log reduction in biofilm dens...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Corbin BD,McLean RJ,Aron GM

    更新日期:2001-07-01 00:00:00

  • Factors affecting rate of methane formation from acetic acid by enriched methanogenic cultures.

    abstract::A stable enrichment culture converting acetic acid to methane was successfully obtained from a pear waste digester, using a synthetic substrate solution with acetic acid as the main carbon source. This enrichment culture converted up to 10 mmol of acetic acid per litre per day at 35 degrees C and did not use hydrogen ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-194

    authors: van den Berg L,Patel GB,Clark DS,Lentz CP

    更新日期:1976-09-01 00:00:00

  • Antioxidant, antihypertensive, and antibacterial properties of endophytic Pestalotiopsis species from medicinal plants.

    abstract::Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-070

    authors: Tejesvi MV,Kini KR,Prakash HS,Subbiah V,Shetty HS

    更新日期:2008-09-01 00:00:00

  • Soil microbial communities of three grassland ecosystems in the Bayinbuluke, China.

    abstract::The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2017-0585

    authors: Shao K,Gao G

    更新日期:2018-03-01 00:00:00

  • An initial test of a method for the estimation of real mean particle size from shadowed samples.

    abstract::During shadowing, a "cap" of metal develops on small particles. This cap increases apparent particle with (measured normal to the shadowing direction) by an extent which cannot be predetermined. The extent of this increase in particle size (here defined as the "cap," X) is estimated in the present method by using oppo...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m85-114

    authors: Willison JH

    更新日期:1985-07-01 00:00:00

  • Deoxyribonucleic acid studies in the genus Caryophanon.

    abstract::The deoxyribonucleic acid (DNA) of 36 isolates of Caryophanon latum and 3 isolates designated Caryophanon tenue, obtained from geographically diverse regions, has been examined for guanine plus cytosine (mol % G+C) base composition, genome size, and for relative reassociation levels. The isolates were found to be homo...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-195

    authors: Adcock KA,Seidler RJ,Trentini WC

    更新日期:1976-09-01 00:00:00

  • Occurrence of both subspecies of Photobacterium damselae in mullets collected in the river Magra (Italy).

    abstract::The natural reservoirs and biological characteristics of pathogenic populations of both subspecies of Photobacterium damselae in aquatic habitants remain unclear because of difficulties in obtaining pathogenic strains from the environment. In the present study, we assessed the occurrence of Photobacterium damselae sub...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-021

    authors: Serracca L,Ercolini C,Rossini I,Battistini R,Giorgi I,Prearo M

    更新日期:2011-05-01 00:00:00

  • Investigations of fungal diversity in wooden structures and soils at historic sites on the Antarctic Peninsula.

    abstract::Investigations of microbial diversity in Antarctic are important to begin to understand ecosystem functioning and decomposition processes. This study documents fungi at 9 historic sites on the Antarctic Peninsula collected from wooden structures, other organic materials, and soils during a joint National Science Found...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W08-120

    authors: Arenz BE,Blanchette RA

    更新日期:2009-01-01 00:00:00

  • Characterization of arsenic-resistant bacteria from the rhizosphere of arsenic hyperaccumulator Pteris vittata.

    abstract::Arsenic hyperaccumulator fern Pteris vittata L. produces large amounts of root exudates that are hypothesized to solubilize arsenic and maintain a unique rhizosphere microbial community. Total heterotrophic counts on rich or defined media supplemented with up to 400 mmol/L of arsenate showed a diverse arsenate-resista...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-005

    authors: Huang A,Teplitski M,Rathinasabapathi B,Ma L

    更新日期:2010-03-01 00:00:00

  • The use of functional analysis of the ribosome as a tool to determine archaebacterial phylogeny.

    abstract::Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-021

    authors: Amils R,Ramírez L,Sanz JL,Marín I,Pisabarro AG,Ureña D

    更新日期:1989-01-01 00:00:00

  • Evaluation of the antiserum agar method for the serogroup identification of Neisseria meningitidis.

    abstract::The antiserum agar method (ASA), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of Neisseria meningitidis. Four hundred meningococcal stains were serogrouped by ASA employing horse antise...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-113

    authors: Ashton FE,Ryan A,Diena BB,Frasch CE

    更新日期:1979-06-01 00:00:00

  • Distribution of 3-hydroxy oxylipins and acetylsalicylic acid sensitivity in Cryptococcus species.

    abstract::Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed tha...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w07-116

    authors: Sebolai OM,Pohl CH,Botes PJ,van Wyk PW,Mzizi R,Swart CW,Kock JL

    更新日期:2008-02-01 00:00:00

  • Cyst and germ tube wall structure in Aphanomyces astaci, Oomycetes.

    abstract::Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-210

    authors: Nyhlén L,Unestam T

    更新日期:1978-11-01 00:00:00

  • Discriminant analysis of microcalorimetric data of bacterial growth.

    abstract::In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-186

    authors: Bermúdez J,López D,Sanahuja A,Viñas M,Lorén JG

    更新日期:1988-09-01 00:00:00

  • Exopolysaccharide depolymerases induced by Rhizobium bacteriophages.

    abstract::Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-239

    authors: Barnet YM,Humphrey B

    更新日期:1975-10-01 00:00:00

  • Bacteriophage lambda repressor allelic modulation of the Rex exclusion phenotype.

    abstract::The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-re...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-021

    authors: Slavcev RA,Bull HJ,Hayes S

    更新日期:2003-03-01 00:00:00

  • Study of characteristics on metabolism of Penicillium chrysogenum F1 during bioleaching of heavy metals from contaminated soil.

    abstract::Penicillium chrysogenum F1 is very efficient in bioleaching heavy metals from the soil and is used for that purpose. We found that F1 can extract 19.8 mg Cd, Cu, Pb, and Zn from 2.5 g soil; the total heavy metals' bioleaching ratio was 60.4%. In this study, the bioleaching mechanism was investigated by means of metabo...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0624

    authors: Deng X,Yang Z,Chen R

    更新日期:2019-09-01 00:00:00

  • Accelerated detection of lymphocytic choriomeningitis virus in diagnostic specimens.

    abstract::Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-245

    authors: Lewis VJ,Thacker WL,Mitchell SH

    更新日期:1976-11-01 00:00:00

  • Biological control of fusarial wilt of pigeon pea by Bacillus brevis.

    abstract::A virulent strain of pigeon pea wilt pathogen was isolated from wilted pigeon pea plants and was identified as Fusarium oxysporum f. sp. udum. Many bacterial cultures showing antagonism to the pathogen were isolated from various ecological niches. When tested under pot and field conditions, development of fusarial wil...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w99-109

    authors: Bapat S,Shah AK

    更新日期:2000-02-01 00:00:00

  • Environmental factors affecting the antagonism of Pseudomonas cepacia against Trichoderma viride.

    abstract::Antagonistic activity of the bacterium Pseudomonas cepacia against Trichoderma viride was greatly influenced by nutritional and environmental conditions. Xylose and trehalose strongly enhanced the antifungal activity of P. cepacia, whereas mannitol and glucose had little effect. The carbon sources that enhanced the an...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m91-152

    authors: Upadhyay RS,Visintin L,Jayaswal RK

    更新日期:1991-11-01 00:00:00

  • Site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide.

    abstract::The site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide was assessed by collecting high-resolution phosphorus nuclear magnetic resonance spectra in the presence of manganese, a paramagnetic divalent cation. This technique revealed high-affinity interactions between the cation and all o...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-010

    authors: Ferris FG,Beveridge TJ

    更新日期:1986-01-01 00:00:00

  • Purification and characterization of a psychrophilic catalase from Antarctic Bacillus.

    abstract::Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-066

    authors: Wang W,Sun M,Liu W,Zhang B

    更新日期:2008-10-01 00:00:00