Synchronous sporulation during idiophase development in surface cultures of Rhizopus arrhizus (Fischer).

Abstract:

:A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicates three metabolic phases. The first is associated with spore germination; the second, trophophase, with growth; and the third, idiophase, with sporulation. Synchronous sporulation and the occurrence of distinct metabolic phases are taken as indications of fairly uniform cell development in the surface cultures. A photographic method of estimating large numbers of fruiting structures on semisolid medium is also described.

journal_name

Can J Microbiol

authors

Lawler GC,Weber DJ

doi

10.1139/m77-151

subject

Has Abstract

pub_date

1977-08-01 00:00:00

pages

1018-25

issue

8

eissn

0008-4166

issn

1480-3275

journal_volume

23

pub_type

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