Abstract：
:The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid analysis over 7-day time course experiments. Cultures were exposed to 20 g/L Bow River crude oil with and without 0.625 g/L Igepal CO-630 (a nonylphenol ethoxylate surfactant), 5 g/L saturates, 5 g/L aromatics, or 125 g/L refinery sludge. A group of six genera dominated the cultures: Acinetobacter, Alcaligenes, Ochrobactrum, Pseudomonas/Flavimonas, Stenotrophomonas, and Yersinia. Species from four of the genera were shown to be capable of hydrocarbon degradation, and counts of hydrocarbon degrading and total heterotrophic bacteria over time were nearly identical. Pseudomonas/Flavimonas and Stenotrophomonas normally dominated during the early portions of cultures, although the lag phase of Stenotrophomonas appears to have been increased by surfactant addition. Acinetobacter calcoaceticus was the most frequently isolated microorganism during exposure to the saturate fraction of crude oil. Regardless of substrate, the culture medium supported a greater variety of organisms during the latter portions of cultures. Understanding the community structure and dynamics of mixed bacterial cultures involved in treatment of heterogeneous waste substrates may assist in process development and optimization studies.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Van Hamme JD,Odumeru JA,Ward OPsubject
Has Abstractpub_date
2000-05-01 00:00:00pages
441-50issue
5eissn
0008-4166issn
1480-3275journal_volume
46pub_type
杂志文章abstract：:The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-049
更新日期：1986-03-01 00:00:00
abstract：:Clinical isolates of Staphylococcus aureus were found to exhibit strain-specific heterogeneity to the growth-enhancing effects of human urokinase (UK), a proteinase with plasminogen activator activity. Nine out of fourteen (64%) methicillin-sensitive strains of S. aureus were responsive to UK in "in vitro" cultures. I...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-131
更新日期：1996-10-01 00:00:00
abstract：:Bacillus subtilis fmbR-1 was obtained from wild-type B. subtilis fmbR by replacement of the native promoter of the surfactin operon with the inducible promoter Pspac. The recombinant B. subtilis fmbR-1 produced more antibacterial substances than the wild-type strain. The overproducing phenotype was related to the enha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-044
更新日期：2009-08-01 00:00:00
abstract：:Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-015
更新日期：1989-01-01 00:00:00
abstract：:Campylobacter spp. are a substantial cause of gastroenteritis worldwide. Human infection can result from ingestion of contaminated food or water from a variety of sources, including the consumption of fresh produce that is contaminated with the pathogen via the use of contaminated irrigation water. Using molecular met...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0710
更新日期：2016-05-01 00:00:00
abstract：:Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W06-132
更新日期：2007-02-01 00:00:00
abstract：:In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-186
更新日期：1988-09-01 00:00:00
abstract：:Two rod-shaped viruses of similar length occurring in hops were isolated from a mixed infection. One (hop mosaic virus) infected Nicotiana clevelandii, produced mosaic symptoms in mosaic-sensitive hop cultivars, and did not infect Chenopodium murale. The other (hop latent virus) infected C. murale but not N. cleveland...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-168
更新日期：1976-08-01 00:00:00
abstract：:A purified lipopolysaccharide (LPS) isolated from the rickettsial agent of Q fever, Coxiella burnetii phase 1, possesses some of the in vivo and in vitro biological properties previously only associated with bacterial endotoxins. The Coxiella LPS is mitogenic for guinea pig leukocytes, induces non-specific resistance ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-259
更新日期：1978-12-01 00:00:00
abstract：:A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-151
更新日期：1979-09-01 00:00:00
abstract：:Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Using a mixture of three mono nitrophenols as sole carbon, nitrogen and energy sources, mixed cultures were enriched from municipal activated sludge to degrade both nitrophenols and nitrobenzene. Bacterial growth and degradation rate could be increased by supplementing the medium with 0.1% YE. Microorganisms were isol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:The presence of other soil microorganisms might influence the ability of rhizobacterial inoculants to promote plant growth either by reducing contact between the inoculant and the plant root or by interfering with the mechanism(s) involved in rhizobacterially mediated growth promotion. We conducted the following exper...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-080
更新日期：2001-09-01 00:00:00
abstract：:We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N', N'-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-040
更新日期：2005-08-01 00:00:00
abstract：:Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-102
更新日期：1990-08-01 00:00:00
abstract：:Chloroquine increases the inhibition of cultured lymphocytes by high concentrations of phytohemagglutinin (PHA) or concanavalin A (Con A). The inhibition is also increased by complement. Thus chloroquine and complement have similar effects. Time-course studies show that chloroquine increases the rate of onset of the c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-231
更新日期：1975-10-01 00:00:00
abstract：:The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically imp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0239
更新日期：2014-07-01 00:00:00
abstract：:For the recently described serotype 15 of biotype I and serotypes 13 and 14 of biotype II of Actinobacillus pleuropneumoniae, fhuA and hgbA were detected by polymerase chain reaction and DNA sequencing. To determine the substrate specificity of the iron receptors FhuA and HgbA and to study their role in the virulence ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-135
更新日期：2006-04-01 00:00:00
abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-102
更新日期：2007-01-01 00:00:00
abstract：:A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydosp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-093
更新日期：1975-05-01 00:00:00
abstract：:The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-079
更新日期：2003-10-01 00:00:00
abstract：:Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which cor...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-071
更新日期：1990-06-01 00:00:00
abstract：:Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-210
更新日期：1978-11-01 00:00:00
abstract：:Pseudomonas maltophilia has been identified in the rhizosphere of several cultured plants: cabbage, rape, mustard, corn, beet. In comparison with nonrhizospheric soil, all the rhizospheric samples analysed contain more of this Pseudomonas and the most important stimulation is supplied by cruciferous plants. The possib...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0239
更新日期：2021-01-01 00:00:00
abstract：:Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate the super-nodulating pea mutant...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2017-0281
更新日期：2017-11-01 00:00:00
abstract：:Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 microgram of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as ca...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-029
更新日期：1979-02-01 00:00:00
abstract：:This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-001
更新日期：1983-01-01 00:00:00
abstract：:A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-155
更新日期：1977-08-01 00:00:00
