Abstract：
:The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid analysis over 7-day time course experiments. Cultures were exposed to 20 g/L Bow River crude oil with and without 0.625 g/L Igepal CO-630 (a nonylphenol ethoxylate surfactant), 5 g/L saturates, 5 g/L aromatics, or 125 g/L refinery sludge. A group of six genera dominated the cultures: Acinetobacter, Alcaligenes, Ochrobactrum, Pseudomonas/Flavimonas, Stenotrophomonas, and Yersinia. Species from four of the genera were shown to be capable of hydrocarbon degradation, and counts of hydrocarbon degrading and total heterotrophic bacteria over time were nearly identical. Pseudomonas/Flavimonas and Stenotrophomonas normally dominated during the early portions of cultures, although the lag phase of Stenotrophomonas appears to have been increased by surfactant addition. Acinetobacter calcoaceticus was the most frequently isolated microorganism during exposure to the saturate fraction of crude oil. Regardless of substrate, the culture medium supported a greater variety of organisms during the latter portions of cultures. Understanding the community structure and dynamics of mixed bacterial cultures involved in treatment of heterogeneous waste substrates may assist in process development and optimization studies.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Van Hamme JD,Odumeru JA,Ward OPsubject
Has Abstractpub_date
2000-05-01 00:00:00pages
441-50issue
5eissn
0008-4166issn
1480-3275journal_volume
46pub_type
杂志文章abstract：:Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modifica...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-103
更新日期：1975-05-01 00:00:00
abstract：:Pathogenic and nonpathogenic isolates of Venturia inaequalis were grown in liquid culture. Hyphae were treated with two types of fimbrial antiserum (AU- and AV-1) and examined by immunofluorescent microscopy, in order to establish the distribution of fimbrial epitopes in whole cell mounts. The AV-1 antiserum was speci...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-061
更新日期：2000-09-01 00:00:00
abstract：:Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-058
更新日期：1993-04-01 00:00:00
abstract：:The respiration of Candida lipolytica on n-tetradecane is decreased in the presence of bentonite. This inhibition is less pronounced by the introduction of hydrophobic chains on the bentonite. Oxygen demand of resting cells varies with the length of hydrophobic chains. In fermentor, addition of adsorbant does not stim...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:A purified lipopolysaccharide (LPS) isolated from the rickettsial agent of Q fever, Coxiella burnetii phase 1, possesses some of the in vivo and in vitro biological properties previously only associated with bacterial endotoxins. The Coxiella LPS is mitogenic for guinea pig leukocytes, induces non-specific resistance ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-259
更新日期：1978-12-01 00:00:00
abstract：:Virulent strains of the fish pathogen Aeromonas salmonicida, which have surface S-layers (S+), efficiently adhere to, enter, and survive within macrophages. Here we report that S+ bacteria were 10- to 20-fold more adherent to non-phagocytic fish cell lines than S-layer-negative (S-) mutants. When reconstituted with ex...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-034
更新日期：2000-07-01 00:00:00
abstract：:Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-209
更新日期：1983-10-01 00:00:00
abstract：:The yeast Saccharomyces cerevisiae Y222 was studied in the presence of the following iron-binding agents: Desferal, dipyridyl, and human and bovine transferrins. We report that cell growth and lanosterol 14 alpha-demethylase cytochrome P450 are not affected by Desferal but that dipyridyl and serum transferrins decreas...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-156
更新日期：1989-10-01 00:00:00
abstract：:Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, G...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-071
更新日期：2010-10-01 00:00:00
abstract：:Colletotrichum gloeosporioides is the causal agent of rubber tree anthracnose and leads to serious losses of natural rubber production. The pathogenesis of C. gloeosporioides on rubber tree remains unknown. Cerato-platanin proteins are small, secreted cysteine-rich proteins that contribute to virulence and function in...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0087
更新日期：2018-11-01 00:00:00
abstract：:Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-041
更新日期：1978-03-01 00:00:00
abstract：:Penicillium chrysogenum F1 is very efficient in bioleaching heavy metals from the soil and is used for that purpose. We found that F1 can extract 19.8 mg Cd, Cu, Pb, and Zn from 2.5 g soil; the total heavy metals' bioleaching ratio was 60.4%. In this study, the bioleaching mechanism was investigated by means of metabo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0624
更新日期：2019-09-01 00:00:00
abstract：:Fungal cultures were grown by a new microslide method and by the traditional slide-culture method. Sporulation results were similar by both methods. The microslide method has the advantages of increased safety to the user and ease of storage. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-204
更新日期：1978-10-01 00:00:00
abstract：:The antimicrobial activity of Ag, Cu, and layered Ag-Cu surface films, sputter-coated onto several types of catheter material, against clinical isolates of Staphylococcus epidermidis and Staphylococcus aureus was evaluated. When 20 microL of a suspension of S. epidermidis or S. aureus (2.68 x 10(6) colony-forming unit...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-134
更新日期：1993-09-01 00:00:00
abstract：:Urease activity was measured using whole cells of both long (swarming) and short (nonswarming) populations of Proteus mirabilis from casein hydrolysate agar (CHA) and broth (CHB) cultures, and from brain heart infusion broth (BHIB) cultures. Urease is a constitutive enzyme for both long and short cells, but its activi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-051
更新日期：1987-04-01 00:00:00
abstract：:Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-051
更新日期：1982-03-01 00:00:00
abstract：:To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hyp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0755
更新日期：2014-03-01 00:00:00
abstract：:A defined medium (CDM) is described which supported growth and sporulation of type E strains of Clostridium botulinum, but not sporulation of other serotypes of C. botulinum or C. sporogenes. As compared to growth in complex medium, spore outgrowth was delayed and both the growth rate and the cell yield was reduced. H...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-076
更新日期：1979-04-01 00:00:00
abstract：:To develop a library-dependent method of tracking fecal sources of contamination of beaches on the Atlantic coast of southwestern France, a library of 6368 Escherichia coli isolates was constructed from samples of feces, from 40 known human or animal sources collected in the vicinity of Arcachon Bay in 2010, and in Fr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0244
更新日期：2020-12-01 00:00:00
abstract：:Citrobacter freundii from an environmental source displayed resistance to a wide variety of antibacterials. Some of these resistances could be transferred by conjugation with frequencies as high as 10(-2). The complexity of plasmid involvement in the antibacterial resistance of this species was demonstrated by isolati...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-052
更新日期：1981-03-01 00:00:00
abstract：:Cultures of Streptomyces peucetius often were observed to divert spontaneously from the production of anthracycline antibiotics to the excretion of acidic metabolites, whether grown in a defined medium or a nutrient-rich medium. The predominant excreted acidic metabolites were determined by high-performance liquid chr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-198
更新日期：1987-12-01 00:00:00
abstract：:Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Boron (B) is toxic to living cells at levels above a certain threshold. We isolated several B-tolerant bacterial strains from soil samples and studied them for possible mechanisms of B tolerance. 16S rRNA gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 gen...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-106
更新日期：2010-01-01 00:00:00
abstract：:The oligosaccharides of human lactoferrin were enzymatically removed with glycopeptidase F, resulting in a preparation containing partial and fully deglycosylated human lactoferrin. The derivatives were separated by Concanavalin A affinity chromatography and compared with native human lactoferrin with respect to their...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-198
更新日期：1992-11-01 00:00:00
abstract：:To facilitate the recognition of plant viruses with similar characteristics a dendrogram of characterized viruses was constructed. The sequence of criteria included: type of nucleic acid; single or double stranded; presence or absence of lipid envelope; helical or nonhelical symmetry; and divided or single genome. Non...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-197
更新日期：1977-09-01 00:00:00
abstract：:A dairy product processing plant was studied for 2.5 years to examine contamination with Staphylococcus aureus and try to correlate the source of contamination. Cultures were submitted to an antibiotic susceptibility test (AST) and characterised by Pulsed-field Gel Electrophoresis (PFGE) analysis. Results showed that ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-111
更新日期：2000-12-01 00:00:00
abstract：:An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-042
更新日期：1976-02-01 00:00:00
abstract：:The N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain Marburg has been purified with reasonable yield and much higher specific activity than previously reported. For the first time it has been shown that both N5,N10-methylenetetrahydromethanopterin dehydrogenase and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-077
更新日期：1989-04-01 00:00:00
abstract：:Methanospirillum hungatei strains GP1 and JF1 when exposed to the adenosinetriphosphatase inhibitor N,N'-dicyclohexylcarbodiimide experienced a marked decline in growth rates, methane synthesis activities, and intracellular ATP concentrations. Although growth was inhibited, the intracellular ATP concentrations of all ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-146
更新日期：1982-08-01 00:00:00
abstract：:Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agri...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-123
更新日期：2009-03-01 00:00:00
